THE INFLUENCE OF AUTOLYSIS ON THE PROTEIN-PEPTIDE PROFILE OF Bos taurus AND Sus scrofa HEART AND AORTA TISSUES


https://doi.org/10.21323/2414-438X-2016-1-2-4-9

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Abstract

The article presents the results of autolytic processes impact on the protein-peptide profile of Bos taurus and Sus scrofa cardiac muscle and aorta. The results of tissue-specific protein identification are also presented as well as the effect of autolysis. Apolipoprotein A-1 involved in the formation of high-density lipoproteins, peroxiredoxin-1 involved in the suppression of oxidative stress, galectin-1 induced apoptosis of T-lymphocytes, as well as number of heat shock proteins with molecular weight less than 30 kDa were identified in Sus scrofa aorta tissue. It was discovered that functional proteins with molecular weight less than 30 kDa are retained during the freezing process, but destroyed under the action of autolytic enzymes. This work was supported by the Russian Science Foundation (project No. 16–16–10073).


About the Authors

I. M. Chernukha
The V.M. Gorbatov All-Russian Meat Research Institute, Moscow
Russian Federation

doctor of technical sciences, professor, leading research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin

109316, Moscow, Talalikhina str., 26

Ph.: 8 (495) 676–97–18



L. V. Fedulova
The V.M. Gorbatov All-Russian Meat Research Institute, Moscow
Russian Federation

candidate of technical sciences, head of Experimental clinic — laboratory of biologically active substances of an animal origin

109316, Moscow, Talalikhina str., 26

Ph.: 8 (495) 676–92–11



E. A. Kotenkova
The V.M. Gorbatov All-Russian Meat Research Institute, Moscow
Russian Federation

candidate of technical sciences, research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin

109316, Moscow, Talalikhina str., 26

Ph.: 8 (495) 676–92–11



S. S. Shishkin
Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow
Russian Federation

doctor of biological sciences, professor, head of the biomedical research laboratory

119071, Moscow, Leninsky prospekt, 33 bldg. 2

Ph.: +7 (495) +952–58–86



L. I. Kovalyov
Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow
Russian Federation

doctor of biological sciences, lead- ing research scientist of the biomedical research laboratory

119071, Moscow, Lenin- sky prospekt, 33 bldg. 2

Ph.: +7 (495) +952–58–86



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Supplementary files

For citation: Chernukha I.M., Fedulova L.V., Kotenkova E.A., Shishkin S.S., Kovalyov L.I. THE INFLUENCE OF AUTOLYSIS ON THE PROTEIN-PEPTIDE PROFILE OF Bos taurus AND Sus scrofa HEART AND AORTA TISSUES. Theory and practice of meat processing. 2016;1(2):4-9. https://doi.org/10.21323/2414-438X-2016-1-2-4-9

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