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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">meat</journal-id><journal-title-group><journal-title xml:lang="en">Theory and practice of meat processing</journal-title><trans-title-group xml:lang="ru"><trans-title>Теория и практика переработки мяса</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2414-438X</issn><issn pub-type="epub">2414-441X</issn><publisher><publisher-name>ФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2414-438X-2016-1-2-4-9</article-id><article-id custom-type="elpub" pub-id-type="custom">meat-14</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>THE INFLUENCE OF AUTOLYSIS ON THE PROTEIN-PEPTIDE PROFILE OF Bos taurus AND Sus scrofa HEART AND AORTA TISSUES</article-title><trans-title-group xml:lang="ru"><trans-title>ВЛИЯНИЕ АВТОЛИЗА НА ПРОТЕОМНО-ПЕПТИДНЫЙ ПРОФИЛЬ СЕРДЕЧНОЙ МЫШЦЫ И АОРТЫ Bos taurus и Sus scrofa</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чернуха</surname><given-names>И. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Chernukha</surname><given-names>I. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Экспериментальная клиники-лаборатория биологически активных веществ животного происхождения</p><p>доктор технических наук, профессор, ведущий научный сотрудник </p><p>109316, г. Москва, ул. Талалихина,26</p><p>Тел.: 8 (495) 676–97–18</p></bio><bio xml:lang="en"><p>doctor of technical sciences, professor, leading research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin</p><p>109316, Moscow, Talalikhina str., 26</p><p>Ph.: 8 (495) 676–97–18</p></bio><email xlink:type="simple">imcher@inbox.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Федулова</surname><given-names>Л. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Fedulova</surname><given-names>L. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Экспериментальная клиники-лаборатория биологически активных веществ животного происхождения</p><p>кандидат технических наук, заведующий</p><p>109316, г. Мо- сква, ул. Талалихина,26</p><p>Тел.: 8 (495) 676–92–11</p></bio><bio xml:lang="en"><p>candidate of technical sciences, head of Experimental clinic — laboratory of biologically active substances of an animal origin</p><p>109316, Moscow, Talalikhina str., 26</p><p>Ph.: 8 (495) 676–92–11</p></bio><email xlink:type="simple">fedulova@vniimp.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Котенкова</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Kotenkova</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Экспериментальная клиники-лаборатория биологически активных веществ животного происхождения</p><p>кандидат технических наук, научный сотрудник</p><p>109316, г. Мо- сква, ул. Талалихина,26</p><p>Тел.: 8 (495) 676–92–11</p><p> </p></bio><bio xml:lang="en"><p>candidate of technical sciences, research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin</p><p>109316, Moscow, Talalikhina str., 26</p><p>Ph.: 8 (495) 676–92–11</p></bio><email xlink:type="simple">lazovlena92@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шишкин</surname><given-names>С. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Shishkin</surname><given-names>S. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>лаборатория биомедицинских исследований</p><p>доктор биологических наук, профессор, заведующий лабораторией </p><p>119071, г. Москва, Ленинский проспект, д.33, стр. 2</p><p>Тел.: +7 (495) 952–58–86</p></bio><bio xml:lang="en"><p>doctor of biological sciences, professor, head of the biomedical research laboratory</p><p>119071, Moscow, Leninsky prospekt, 33 bldg. 2</p><p>Ph.: +7 (495) +952–58–86</p></bio><email xlink:type="simple">sergeyshishkin@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ковалев</surname><given-names>Л. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Kovalyov</surname><given-names>L. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>лаборатория биомедицинских исследований</p><p>доктор биологических наук, ведущий научный сотрудник лаборатории </p><p>119071, г. Москва, Ленинский проспект, д.33, стр. 2</p><p>Тел.: +7 (495) 952–58–86</p></bio><bio xml:lang="en"><p>doctor of biological sciences, lead- ing research scientist of the biomedical research laboratory</p><p>119071, Moscow, Lenin- sky prospekt, 33 bldg. 2</p><p>Ph.: +7 (495) +952–58–86</p></bio><email xlink:type="simple">kovalyov@inbi.ras.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Всероссийский научно-исследовательский институт мясной промышленности имени В.М. Горбатова»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>The V.M. Gorbatov All-Russian Meat Research Institute, Moscow</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Федеральное государственное учреждение «Федеральный исследовательский центр «Фундаментальные основы биотехнологии» Российской академии наук»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Institution “Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>08</day><month>07</month><year>2016</year></pub-date><volume>1</volume><issue>2</issue><fpage>4</fpage><lpage>9</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Chernukha I.M., Fedulova L.V., Kotenkova E.A., Shishkin S.S., Kovalyov L.I., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Чернуха И.М., Федулова Л.В., Котенкова Е.А., Шишкин С.С., Ковалев Л.И.</copyright-holder><copyright-holder xml:lang="en">Chernukha I.M., Fedulova L.V., Kotenkova E.A., Shishkin S.S., Kovalyov L.I.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.meatjournal.ru/jour/article/view/14">https://www.meatjournal.ru/jour/article/view/14</self-uri><abstract><p>The article presents the results of autolytic processes impact on the protein-peptide profile of Bos taurus and Sus scrofa cardiac muscle and aorta. The results of tissue-specific protein identification are also presented as well as the effect of autolysis. Apolipoprotein A-1 involved in the formation of high-density lipoproteins, peroxiredoxin-1 involved in the suppression of oxidative stress, galectin-1 induced apoptosis of T-lymphocytes, as well as number of heat shock proteins with molecular weight less than 30 kDa were identified in Sus scrofa aorta tissue. It was discovered that functional proteins with molecular weight less than 30 kDa are retained during the freezing process, but destroyed under the action of autolytic enzymes. This work was supported by the Russian Science Foundation (project No. 16–16–10073).</p></abstract><trans-abstract xml:lang="ru"><p>В статье представлены результаты изучения влияния автолитических процессов на белково-пептидный состав сердечной мышцы и аорты Bos taurus и Sus scrofa, приведены результаты идентификации тканеспецифичных белковых молекул и влияние автолиза на их сохранность. В тканях аорты Sus scrofa были обнаружены аполипопротеин А-1, участвующий в образовании липопротеинов высокой плотности, пероксиредоксин-1, участвующий в подавлении окислительного стресса, галектин-1, индуцирующий апоптоз Т-лимфоцитов, а также ряд белков теплового шока, имеющих молекулярную массу менее 30 кДа. Было установлено, что функциональные белковые вещества с  молекулярной массой менее 30 кДа сохраняются в процессе заморозки, но разрушаются под действием ферментов сырья при автолизе. Исследование выполнено за счет гранта Российского научного фонда (проект №16–16–10073).</p></trans-abstract><kwd-group xml:lang="ru"><kwd>аорта</kwd><kwd>тканевая специфичность</kwd><kwd>автолиз</kwd><kwd>электрофорез</kwd></kwd-group><kwd-group xml:lang="en"><kwd>aorta</kwd><kwd>tissue specificity</kwd><kwd>autolysis</kwd><kwd>electrophoresis</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Ковалев Л.И. Протеомное изучение белков в образцах свинины и выработанных из нее мясных продуктах / Л.И. Ковалев, С.С. Шишкин, М.А. Ковалева, А.В. Иванов, Н.Л. Вострикова, И.М. Чернуха // Всё о мясе. — 2013. — №3. — С. 32–34.</mixed-citation><mixed-citation xml:lang="en">Kovalyov L.I. Proteomic study of proteins in pork and meat products / L.I. Kovalyov, S.S. Shishkin, M.A. Kovalyova, A.V. Ivanov, N.L. Vostrikova, I.M Chernukha // All about meat. — 2013. — No. 3. — P. 32–34.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Лисицын А.Б. Ткани сердца и аорты крупного рогатого скота и свиней как функциональный мясной ингредиент с заданным белково-пептидным профилем / А.Б. Лисицын, И.М. Чернуха, Л.В. Федулова, Е.А. Котенкова, С.С. Шишкин // Все о мясе. — №5. — 2013. — C.48–51</mixed-citation><mixed-citation xml:lang="en">Lisitsyn A.B. Cattle and pig heart and aorta tissues as a func- tional food ingredient with a protein-peptide profile / A.B. Lisit- syn, I.M. Chernukha, L.V. Fedulova, E.A. Kotenkova, S.S. Shish- kin // All about meat. — No. 5. — 2013. — P. 48–51</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Чернуха И.М. Влияние тканеспецифичных биомолекул на дисфункцию эндотелия при атеросклерозе / И.М. Чернуха, Л.В. Федулова, Е.А. Котенкова // Все о мясе. — 2016. №1. С. 46–49.</mixed-citation><mixed-citation xml:lang="en">Chernukha I.M. The influence of tissue-specific biomolecules on endothelial dysfunction in atherosclerosis / I.M. Chernukha, L.V. Fedulova, E.A. Kotenkova // All about meat. — 2016. — No. 1. — P. 46–49.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Fagerberg, L. Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Anti- body-based Proteomics/ L. Fagerberg, B.M. Hallstrom, P. Oksvold, C. Kampf, D. Djureinovic, J. Odeberg, M. Habuka, S. Tahmaseb- poor, A.Danielsson, K. Edlund, A. Asplund, E. Sjostedt, E. Lund- berg, C. Al-Khalili Szigyarto, M. Skogs, J.O. Takanen, H. Berling, H. Tegel, J. Mulder, P. Nilsson, J. M. Schwenk, C. Lindskog, F. Daniels- son, A. Mardinoglu, Å. Sivertsson, K. von Feilitzen, M. Forsberg, M. Zwahlen, I. Olsson, S. Navani, M. Huss, J. Nielsen, F.Ponten, M. Uhlen // Molecular &amp; Cellular Proteomics. — 2014. — №13. (2). — Р. 397–406.</mixed-citation><mixed-citation xml:lang="en">Fagerberg, L. Analysis of the Human Tissue-specific Ex- pression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics/ L. Fagerberg, B.M. Hallstrom, P. Oksvold, C. Kampf, D. Djureinovic, J. Odeberg, M. Habuka, S. Tahmasebpoor, A.Danielsson, K. Edlund, A. Asplund, E. Sjost- edt, E. Lundberg, C. Al-Khalili Szigyarto, M. Skogs, J.O. Takanen, H. Berling, H. Tegel, J. Mulder, P. Nilsson, J. M. Schwenk, C. Lind- skog, F. Danielsson, A. Mardinoglu, Å. Sivertsson, K. von Feilitzen, M. Forsberg, M. Zwahlen, I. Olsson, S. Navani, M. Huss, J. Nielsen, F.Ponten, M. Uhlen // Molecular &amp; Cellular Proteomics. — 2014. — No. 13 (2). — Р. 397–406.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Handbook of Meat and Meat Processing, Second Edition/ed- ited Y. H. Hui //USA: Taylor &amp; Francis Group. — 2012. — 1000 p. 6. Kovalyov L.I. Polymorphism of delta3,5-delta2,4-dienoyl-co- enzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue / L.I. Kovalyov, M.A. Kovalyova, P.L. Kovalyov, M.V. Serebryakova, S.A. Moshkovskii, S.S. Shishkin // Biochemistry (Moscow). — 2006. — V.71. — №4. — Р. 448–453.</mixed-citation><mixed-citation xml:lang="en">Handbook of Meat and Meat Processing, Second Edition/ed- ited Y. H. Hui //USA: Taylor &amp; Francis Group. — 2012. — 1000 P.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Laemmli U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / U.K. Laemmli // Nature. — 1970. — V.227. — №5259. — P.680–685.</mixed-citation><mixed-citation xml:lang="en">Kovalyov L.I. Polymorphism of delta3,5-delta2,4-dienoyl-co- enzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue / L.I. Kovalyov, M.A. Kovalyova, P.L. Kovaly- ov, M.V. Serebryakova, S.A. Moshkovskii, S.S. Shishkin // Biochemistry (Moscow). — 2006. — V. 71. — No. 4. — Р. 448–453.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Lafarga T. Bioactive peptides from meat muscle and byproducts: generation, functionality and application as functional ingredients/ T. Lafarga, M. Hayes// Meat science. — 2014. — №98. — р. 227–239.</mixed-citation><mixed-citation xml:lang="en">Laemmli U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / U.K. Laemmli // Nature. — 1970. — V. 227. — No. 5259. — P. 680–685.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Nutraceutical Science and Technology (Book 4). Nutraceutical Proteins and Peptides in Health and Disease/edited by Y. Mine, F. Shahidi//USA: Taylor &amp; Francis Group. — 2006. — 688 p.</mixed-citation><mixed-citation xml:lang="en">Lafarga T. Bioactive peptides from meat muscle and byproducts: generation, functionality and application as functional ingredients / T. Lafarga, M. Hayes // Meat science. — 2014. — No. 98. — P. 227–239.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Udenigwe C. C. Meat proteome as source of functional biopeptides/C. C. Udenigwe, A. Howard//Food Research International. — 2013. — №54. — р. 1021–1032.</mixed-citation><mixed-citation xml:lang="en">Nutraceutical Science and Technology (Book 4). Nutraceutical Proteins and Peptides in Health and Disease/edited by Y. Mine, F. Shahidi//USA: Taylor &amp; Francis Group. — 2006. — 688 P.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Xu Y. Differential proteome and transcriptome analysis of porcine skeletal muscle during development/ Y. Xu, H. Qian, X. Feng, Y. Xiong, M. Lei, Z. Ren, B. Zuo, D. Xu, Y. Ma, H. Yuan// Journal of Proteomics. — 2012. — V.75(7). — P.2093–2108.</mixed-citation><mixed-citation xml:lang="en">Udenigwe C. C. Meat proteome as source of functional biopeptides/C. C. Udenigwe, A. Howard//Food Research International. — 2013. — No. 54. — P. 1021–1032.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Xu Y. Differential proteome and transcriptome analysis of porcine skeletal muscle during development/ Y. Xu, H. Qian, X. Feng, Y. Xiong, M. Lei, Z. Ren, B. Zuo, D. Xu, Y. Ma, H. Yuan// Journal of Proteomics. — 2012. — V. 75(7). — P. 2093–2108.</mixed-citation><mixed-citation xml:lang="en">Xu Y. Differential proteome and transcriptome analysis of porcine skeletal muscle during development/ Y. Xu, H. Qian, X. Feng, Y. Xiong, M. Lei, Z. Ren, B. Zuo, D. Xu, Y. Ma, H. Yuan// Journal of Proteomics. — 2012. — V. 75(7). — P. 2093–2108.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru"></mixed-citation><mixed-citation xml:lang="en"></mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
