IDENTIFICATION OF ENTEROTOXIGENIC STAPHYLOCOCCI IN MEAT RAW MATERIALS


https://doi.org/10.21323/2414-438X-2016-1-4-19-27

Full Text:


Abstract

The need to renew the safety criteria for meat raw material used in production of fermented sausages was determined. The presence of the enterotoxigenic S. aureus strains in meat raw material was established. The method was mastered and the PCR approaches to identification and screenings of enterotoxigenic strains and their toxins in meat raw material were proposed. In order to identify enterotoxigenicity of detected staphylococci, the conservative regions of the S. aureus target gene sequences responsible for production of different types of enterotoxins (A, B, Е, С, D) were found and studied. In addition, short fragments of a nuclear acid (primers) corresponding to these revealed genes were constructed. As a result of identification, it was established that two isolated strains of S. аureus were enterotoxigenic. One of them produced type А and Е toxins (strain NG1) and another produced type С and Е toxins (strain NG2). The sensitivity and specificity of the real-time PCR method allowed not only identification of pure cultures but also screening of enterotoxigenic strains and their toxins in a product. The use of this method in the production control for the presence of the enterotoxigenic strains of S. aureus in meat raw material used in fermented sausage manufacture was recommended.


About the Authors

D. S. Bataeva
V.M. Gorbatov All-Russian Meat Research Institute,
Russian Federation

Bataeva Dagmara Sultanovna  — candidate of technical sciences, docent, Head of the Direction of Microbiology, leading scientific worker of the Laboratory «Hygiene of production and microbiology»,

109316, Moscow, Talalikhina str., 26



M. Yu. Minaev
V.M. Gorbatov All-Russian Meat Research Institute,
Russian Federation

Minaev Mikhail Yur’evich — candidate of technical sciences, Head of the Direction of PCR, leading scientific worker of the Laboratory «Hygiene of production and microbiology»,

109316, Moscow, Talalikhina str., 26



A. A. Makhova
V.M. Gorbatov All-Russian Meat Research Institute,
Russian Federation

Makhova Anzhelika Alexandrovna — junior researcher of the Laboratory «Hygiene of production and microbiology»,

109316, Moscow, Talalikhina str., 26



References

1. Sarkar A.A. Antimicrobial resistance and virulence markers in methicillin sensitive Staphylococcus aureus isolates associated with nasal colonization // A. Sarkar, A. Raji, G. Garaween, O. Soge, J. Rey-Ladino, W. Al-Kattan, A. Shibl, A. Senok — Microbial Pathogenesis. — 2016. — V. 93. — № 4. — P. 8–12.

2. Cremonesi P. Genomic characteristics of Staphylococcus aureus strains associated with high within-herd prevalence of intramammary infections in dairy cows // P. Cremonesi, F. Pozzi, M. Raschetti, G. Bignoli, E. Capra, H.U. Graber, F. Vezzoli, R. Piccinini, B. Bertasi, S. Biffani, B. Castiglioni, M. Luini — Journal of Dairy Science. — 2015. — V. 98. — № 10. — P. 6828–6838.

3. Bar-Gal G.K. Host-specificity of Staphylococcus aureus causing intramammary infections in dairy animals assessed by genotyping and virulence genes // G.K. Bar-Gal, S.E. Blum, L. Hadas, R. Ehricht, S. Monecke, G. Leitner — Veterinary Microbiology. — 2015. — V. 176. — № 1–2. — P. 143–154.

4. Budd K.E. Lineage associated expression of virulence traits in bovine-adapted Staphylococcus aureus // K.E. Budd, J. Mitchell, O.M. Keane — Veterinary Microbiology. — 2016. — V. 189. — № 6. — P. 24–31.

5. Bardiau M., Caplin J., Detilleux J., Graber H., Moroni P., Taminiau B., Mainil J.G. Existence of two groups of Staphylococcus aureus strains isolated from bovine mastitis based on biofilm formation, intracellular survival, capsular profile and agr-typing // M. Bardiau, J. Caplin, J. Detilleux, H. Graber, P. Moroni, B. Taminiau, J.G. Mainil — Veterinary Microbiology. — 2016. — V. 185. — № 3. — P. 1–6.

6. Lundberg A. Udder infections with Staphylococcus aureus, Streptococcus dysgalactiae, and Streptococcus uberis at calving in dairy herds with suboptimal udder health // A. Lundberg, A.K. Nyman, A. Aspan, S. Borjesson, H.E. Unnerstad, K.P. — Waller Journal of Dairy Science. — 2016. — V. 99. — № 3. — P. 2102–2117.

7. Monecke S. Genotyping of Staphylococcus aureus isolates from diseased poultry // S. Monecke, A. Ruppelt, S. Wendlandt, S. Schwarz, P. Slickers, R. Ehricht, S.C. de Jäckel — Veterinary Microbiology. — 2013. — V. 162. — № 2–4. — P. 806–812.

8. Marianski A. Meat Smoking and smokehouse design // A. Marianski, R. Marianski, S. Marianski. — British Columbia: English. Publisher. Bookmagic, LLC. — 2009. — P. 324.

9. Marianski A. The art of making fermented sausages // A. Marianski, S. Marianski — Grand Rapids, MI, United States, 2010. — P. 272.

10. Minaev М.Yu., Bataeva D.S., Eremtsova А.А. Technological risks in production of dry fermented sausages // Meat industry. — 2015. — № 12. — P. 24 — 28.

11. http://ncbi.nlm.nih.gov/.

12. Electronic resource www.ncbi.nlm.nih.gov/Blast.

13. Holeckova B., Holoda E., Fotta M., Kalinacova V., Gondol J., Grolmus J. Occurrence of enterotoxigenic Staphylococcus aureus in food. Ann Agric Environ Med. 2002; 9(2): 179–82.

14. Kitai S., Shimizu A., Kawano J., Sato E., Nakano C., Kitagawa H., et al. Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan. J Vet Med Sci. 2005; 67(3): 269–74.13.

15. Imani F.A., Iman I.D., Hosseini D.R., Karami A., Marashi S.M. Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae. Asian Pac J Trop Med. 2013; 6(2): 115–8.


Supplementary files

For citation: Bataeva D.S., Minaev M.Y., Makhova A.A. IDENTIFICATION OF ENTEROTOXIGENIC STAPHYLOCOCCI IN MEAT RAW MATERIALS. Theory and practice of meat processing. 2016;1(4):19-27. https://doi.org/10.21323/2414-438X-2016-1-4-19-27

Views: 358

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2414-438X (Print)
ISSN 2414-441X (Online)