THE INFLUENCE OF BIOACTIVE SUBSTANCES ISOLATED FROM PORCINE AORTA ON THE SERUM FATTY ACID COMPOSITION OF HYPERLIPIDEMIC RATS


https://doi.org/10.21323/2414-438X-2018-3-4-16-22

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Abstract

Based on results of fatty acid composition in serum of hyperlipidemic rats atherogenic index (AI) was calculated. The objects of the study were a commercial bioactive additive (BAA) containing a mixture of peptides isolated from the vessels of farm animals (Scientific and Production center of Revitalization and Health (SPRH), Russia), low molecular weight (LMUF), Mm<5kDa) and medium molecular weight (MMUF), Mm 5–30 kDa) ultrafiltrates of porcine aorta extract. Experimental hyperlipidemia was stimulated in male Wistar rats. After modeling animals in group 2 consumed 0.9 % sodium chloride solution, 3 groups — BAA, 4 groups — LMUF, 5 groups — MMUF, 1 group consisted of intact rats, contained under similar conditions. All studied samples were administered per os in a quantity of 0.3 mg protein / kg body weight for 14 days. As a result of the study, it was noted that in serum of rats treated with BAA there was polyunsaturated fatty acids (PUFA) increase by 67.2 % (P<0.05), while monounsaturated fatty acids (MUFA) was decreased by 29.5 % (P<0.05) compared to the control, but there was no change in AI. Per os administration of LMUF caused a similar effect as BAA: there was an increase in the proportion of PUFA by 2.5 times (p<0.05) compared with control, while MUFA decreased by 39.7 % (P<0.05), mainly due to a reduction of oleic acid by 66.3 % (P<0.05). The relative content of saturated fatty acids (SFA) decreased by 27.8 % (P<0.05), mainly due to reduction of palmitic acid content by 45.3 % (P<0.05) compared to the control, which contributed to a decrease in serum AI by 56.4 % (P<0.05). Per os administration of MMUF did not impact on relative content of MUFA, although the share of oleic acid was reduced by 48.0 % (P<0.05), there was also an increase of PUFA content by 85.8 % (P<0.05) compared with the control, serum AI reduced by 76.9 % (P<0.05) compared with the control group also due to a decrease of palmitic acid by 78.2 % (P<0.05).


About the Authors

Irina M. Chernukha
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation

Doctor of technical sciences, professor, lead­ ing research scientist of Experimental clinic — laboratory «Biologi­cally active substances of an animal origin»

109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–63–21



Liliya V. Fedulova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation

Candidate of technical sciences, head of Experi­mental clinic­laboratory «Biologically active substances of an animal origin» 

109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11



Elena A. Kotenkova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation

Candidate of technical sciences, senior re­search scientist, Experimental clinic­laboratory «Biologically active substances of an animal origin»

109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11



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Supplementary files

For citation: Chernukha I.M., Fedulova L.V., Kotenkova E.A. THE INFLUENCE OF BIOACTIVE SUBSTANCES ISOLATED FROM PORCINE AORTA ON THE SERUM FATTY ACID COMPOSITION OF HYPERLIPIDEMIC RATS. Theory and practice of meat processing. 2018;3(4):16-22. https://doi.org/10.21323/2414-438X-2018-3-4-16-22

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