INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION
https://doi.org/10.21323/2414-438X-2018-3-3-27-35
Abstract
This article highlights the problem of the use of chemical preservatives in the food industry. The prospects of implementation natural substances with an antimicrobial effect for prolonging shelf life and improving the quality of food products are also discussed. Methods for isolating of antimicrobial substances from epithelial and mucous tissues of animal origin are proposed, taking into account the minimization of losses of their biological activity in accordance with the structure of protein-peptide molecules. Based on the results of the determination of antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus and Pseudomonas aeruginosa using flow cytometry, stains Eva Green and PI, the most promising raw materials were determined and the feasibility of using weakly acid extraction followed by trypsinolysis for releasing antimicrobial substances from preprotein molecules and ultrafiltration for purification from high-molecular compounds were investigated.
Supporting agencies: This work was supported by the Russian Science Foundation (project No. 17–76–10033).
About the Authors
Ekaterina A. Lukinova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
senior laboratory assistant, Experimental clinic-laboratory «Biologically active substances of an animal origin»
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
Elena A. Kotenkova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
candidate of technical sciences, senior research scientist, Experimental clinic-laboratory «Biologically active substances of an animal origin»
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
Ekaterina K. Polischuk
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
senior laboratory assistant, Experimental clinic-laboratory «Biologically active substances of an animal origin»
Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
References
1. Lukinova, E.A., Kotenkova, E.A., Makarenko, A.N. (2017). Antimicrobial substances: an alternative approach to the extension of shelf life. Theory and practice of meat processing, 2(3), 4–20. (In Russian)
2. Aslanova, M.A., Dydykin, A.S., Fedulova, L.V., Derevitskaya, O.K. (2017). An effect of the electromagnetic treatment on oxidative stability and microbiological safety of meat semi-prepared products. Theory and practice of meat processing,2(3), 39–48. (In Russian)
3. State policy of the Russian Federation in the field of healthy nutrition. [Electronic resource: URL: https://rmapo.ru/medical/58-osnovy-gosudarstvennoy-politiki-rossiyskoy-federa-cii-v-oblasti-zdorovogo-pitaniya-naseleniya-na-period-do-2020-go-da.html Access data: 04.07.2018]. (In Russian)
4. The Antimicrobial Peptide Database [Electronic resource: URL: http://aps.unmc.edu Access data: 08.06.2018].
5. Kulakova, E.V., Elizarova, V.M., Pampura, A.N. (2012). Endogenous antimicrobial polypeptides — factors of nonspecific protection of the organism. Rossiiskii Stomatologicheskii Zhurnal,6, 42–45. (In Russian)
6. Zasloff, M., Martin, B., Chen, H. — C. (1988). Antimicrobial activity of synthetic magainin peptides and several analogues. Proceedings of the National Academy of Sciences of the United States of America,85(3), 910–913.
7. Ge, Y., Yan, H., Hui, B., Ni, Y., Wang, S., Cai, T. (2002). Extraction of natural vitamin E from wheat germ by supercritical carbon dioxide. Journal of Agricultural and Food Chemistry,50(4), 685–689.
8. Shi, J. (1996). Antibacterial activity of a synthetic peptide (PR-26) derived from PR-39, a proline-arginine-rich neutrophil antimicrobial peptide. Antimicrob. Agents Chemother, 40(1), 115–121.
9. Thouzeau, C., Le Maho, Y., Froget, G., Sabatier, L., Le Bohec, C. Hoffmann, J.A., Bulet, P. (2003). Spheniscins, Avian β-Defensins in Preserved Stomach Contents of the King Penguin, Aptenodytes patagonicus. Journal of Biological Chemistry, 278(51), 51053–51058.
10. Cole, A.M., Weis, P., Diamond, G. (1997). Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. Journal of Biological Chemistry, 272(18), 12008–12013.
11. Mor, A., Nicolas, P. (1994). Isolation and structure of novel defensive peptides from frog skin. European Journal of Biochemistry, 219(1–2), 145–154.
12. Hee Lee, I.N., Cho, Y., Lehrer, R.I.(1997). Effects of pH and salinity on the antimicrobial properties of clavanins. Infection and Immunity, 65(7), 2898–2903.
13. Wilcox, W., Eisenberg, D. (1992). Thermodynamics of melittin tetramerization determined by circular dichroism and implications for protein folding. Protein Science,1(5), 641–653.
14. Ovchinnikova, T.V. (2011). Structural and functional study of natural peptide antibiotics. Dissertation for the scientific degree of Doctor of Chemical Sciences. — M: Shemyakin-Ovchinnikov Institute of bioorganic chemistry. — 68 р. (In Russian)
15. Kokryakov, V.N. (1995). Physico-chemical and functional properties of antimicrobial proteins and peptides. Author’s abstract of the dissertation for the scientific degree of Doctor of Biological Sciences. St. Petersburg: Research Institute of Experimental Medicine RAMS. — 48 р. (In Russian)
16. Shamova, O.V. (1995). Physico-chemical characteristics and functional properties of defensins and proteasins. Author’s abstract of the dissertation for the scientific degree of Candidate of Biological Sciences. St. Petersburg, Research Institute of Experimental Medicine RAMS. — 24 p. (In Russian)
17. Shamova, O.V. (2013). Molecular-cellular bases of realization of biological activity of antimicrobial peptides of leukocytes. Author’s abstract of the dissertation for the scientific degree of Doctor of Biological Sciences. St. Petersburg: Research Institute of Experimental Medicine RAMS. — 48 p. (In Russian)
18. Vasilevskaya, E.R., Fedulova, L.V. (2015). Animal raw materials as a source of natural regulators of immunity. Actual biotechnology, 3(14), 97–98. (In Russian)
19. Zharkova, M.S. (2016). Combined action of proteins and peptides of the system of innate immunity and compounds of different chemical nature in the realization of their antibiotic properties. Author’s abstract of the dissertation for the scientific degree of Candidate of Biological Sciences. St. Petersburg: Research Institute of Experimental Medicine RAMS. — 24 р. (In Russian)
20. Bhat, Z.F., Kumar, S., Bhat, H.F. (2015). Bioactive peptides of animal origin: a review. Journal of Food Science and Technology,52(9), 5377–5392
21. Przybylski, R., Firdaous, L., Châtaigné, G., Dhulster, P., Nedjar, N. (2016). Production of an antimicrobial peptide derived from slaughterhouse by-product and its potential application on meat as preservative. Food Chemistry,211, 306–311.
22. Keska, P., Stadnik, J. (2017). Antimicrobial peptides of meat origin — an in silico and in vitro analysis. Protein and Peptide Letters,24(2), 165–173.
Review
For citations:
Lukinova E.A., Kotenkova E.A., Polischuk E.K. INFLUENCE OF APPROACHES TO ISOLATION OF ANIMAL BIOACTIVE SUBSTANCES ON ANTIMICROBIAL ACTION. Theory and practice of meat processing. 2018;3(3):27-35. https://doi.org/10.21323/2414-438X-2018-3-3-27-35
Views: 569
Email this article 