APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE


https://doi.org/10.21323/2414-438X-2018-3-4-32-37

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Abstract

Eight protein substances that undergo changes during autolysis were found using the electrophoretic methods with the following mass spectrometric identification. The revealed protein substances have different origin: structural proteins (fragments of troponins T and myosin light chains), and metabolic proteins (creatine kinase, pyruvate kinase and alpha-enolase). The decomposition of the fractions of fast skeletal muscle troponin T in 28.0 kDa, 27 kDa and 26.5 kDa was most pronounced.

Identification of constitutive proteins and detection of the products of post — mortem degradation of protein molecules make them suitable candidates for meat quality markers and the following study of these specific fragments will lead to better understanding of the proteolytic activities that take part in the post mortem muscle transformation into meat.


About the Authors

Irina M. Chernukha
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation

Doctor of technical sciences, professor, cor­responding member to the Russian Academy of Sciences, leading re­search scientist, Experimental clinic­-laboratory «Biologically active substances of an animal origin»

109316, Moscow, Talalikhina str., 26
Tel: +7–495–676–63–21



Anastasiya G. Akhremko
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation

Research assistant, Experimental clin­ic-­laboratory «Biologically active substances of an animal origin»

109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11



References

1. ExPASy. SIB Bioinformatics Resource Portal [Electronic resource URL: http://www.expasy.org/proteomics/ Access data: 30.10.2018 г.]

2. Database «Proteomics of the muscular organs» [Electronic resource URL: http://mp.inbi.ras.ru Access data: 30.10.2018 г.]

3. Lametsch, R., Roepstorff, P., Bendixen, E. (2002). Identification of protein degradation during postmortem storage of pig meat. Journal of Agricultural and Food Chemistry, 50(20), 5508–5512.

4. Morzel, M., Chambon, C., Hamelin, M., Santé-Lhoutellier, V., Sayd, T., Monin, G. (2004). Proteome changes during pork meat ageing following use of two different pre-slaughter handling procedures. Meat Science,67(4), 689–696.

5. Bouley, J, Chambon, C, Picard, B. (2004). Mapping of bovine skeletal muscle proteins using two-dimensional gel electrophoresis and mass spectrometry. Proteomics,4(6), 1811– 1824.

6. Doherty, M.K., McLean, L., Hayter, J.R., Pratt, J.M., Robertson, D.H.L., El-Shafei, A., Gaskell, S.J., Beynon, R.J. (2004). The proteome of chicken skeletal muscle: changes in soluble protein expression during growth in a layer strain. Proteomics,4(7), 2082–2093.

7. Kovalyov, L.I., Shishkin, S.S., Kovalyov, M.A., Ivanov, A.V., Vostrikova, N.L., Сhernukha, I.M. (2013). Proteomic research proteins in a sample of pork meat products. Vsyo o myase,3, 32–34. (In Russian)

8. Laemmli, U.K., (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685.

9. O’Farrell, P.H. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry ,250(10), 4007–4021.

10. Chernukha, I.M., Fedulova, L.V., Akhremko, A.G., Kotenkova, E.A. (2017). A comparative study of Sus scrofa m. Longissimus dorsi with different changes in quality. Potravinarstvo Slovak Journal of Food Sciences, 11(1), 398–402.

11. Zamaratskaia, G., Li, S. (2017). Proteomics in meat science-current status and future perspective. Theory and practice of meat processing, 2(1),18–26. (In Russian)

12. Muroya, S., Ohnishi-Kameyama, M., Oe, M., Nakajima, I., Chikuni, K. (2007). Postmortem changes in bovine troponin T isoforms on two-dimensional electrophoretic gel analyzed using mass spectrometry and western blotting: The limited fragmentation into basic polypeptides. Meat Science, 75(3), 506–14


Supplementary files

For citation: Chernukha I.M., Akhremko A.G. APPLICATION OF PROTEOMIC TOOLS: THE AUTOLYTIC CHANGES OF PORK MUSCULAR TISSUE. Theory and practice of meat processing. 2018;3(4):32-37. https://doi.org/10.21323/2414-438X-2018-3-4-32-37

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