CONCEPTUAL APPROACHES TO THE RAPID DETECTION OF CAMPYLOBACTER SPP. IN MEAT OF SLAUGHTER ANIMALS
Abstract
The modern approach to quality assurance of food products based on the ISO 9000 standards indicates the need for the implementation of quality management systems in processing plants. According to the analysis of scientific publication databases (Science Direct and Web of Science), it is established that only 0.5–1.7% of publications are related to studying meat of slaughter animals (except for birds) concerning the presence of Campylobacter. The priority method of investigation is PCR. Ready-to-use PCR test system was developed for the detection of Campylobacter spp. on the basis of selected gene-specific primers to bacteria of Campylobacter genus. Specificity of the test system is established for Gram-negative bacteria of Salmonella, Escherichia, and Proteus genera, and for oxidase-positive Aeromonas. Gene-specific primers for Campylobacter were selected and ready-to-use PCR test system was developed on their basis. It was found that the selected primers have 100% convergence to the genome of Campylobacter genus bacteria, the PCR efficiency is not less than 95%, and the detection limit is not more than 1× 104 CFU/g. When estimating the specificity of the primers, it was taken into account that the bacteria of Campylobacter genus may be incorporated in a consortium with intestine microbiome, mainly with Enterobacteriaceae and lactic acid bacteria. However, Bolton’s enrichment medium is selective and, during the cultivation process, suppresses the growth of Gram-positive lactic acid bacteria. It was found that the selected primers were 100% specific and did not give false positive reactions with this group of microorganisms. The developed test system was successfully validated in a cycle of qualitative tests in the FEPAS system and implemented into laboratory practice. It was proved that the developed test system may be used both in screening at the stages of Campylobacter enrichment and in identification of pure culture of the microorganism.
About the Authors
D. S. Bataeva
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation
Russian Federation
Dagmara S. Bataeva — candidate of technical sciences, docent, Head of the Direction of Microbiology, leading scientific worker of the Laboratory«Hygiene of production and microbiology».
109316, Moscow, Talalikhina str., 26, tel.: +7–495–676–60–11M. Yu. Minaev
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation
Russian Federation
Mikhail Yu. Minaev— candidate of technical sciences, a head of the Molecular diagnostic division, leading scientific worker of the Laboratory«Hygiene of production and microbiology».
109316, Moscow, Talalikhina str., 26, tel.: +-495–676–60–11
Yu. K. Yushina
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation
Russian Federation
Yulia K. Yushina — candidate of technical sciences, docent, Head of the Laboratory «Hygiene of production and microbiology».
109316, Talalikhina str. 26, tel.: +7–495–676–91–26
O. V. Sokolova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation
Russian Federation
Olga V. Sokolova — candidate of technical sciences, junior researcher worker of the Laboratory«Hygiene of production and microbiology».
109316, Moscow, Talalikhina str., 26, tel.: +7–495–676–60–11
E. V. Zajko
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation
Russian Federation
Elena V. Zaiko — senior research technician of the Laboratory«Hygiene of production and microbiology».
109316, Moscow, Talalikhina str., 26, tel.: +7–495–676–60–11
References
1. Campbell, B.J., Engel, A.S., Porter, M.L., Takai, K. (2006). The versatile ε-proteobacteria: Key players in sulphidic habitats. Nature Reviews Microbiology, 4(6), 458–468.
2. WHO publishes list of bacteria for which new antibiotics are urgently needed. Media center of WHO. [Electronic resource: http://www.who.int/mediacentre/news/releases/2017/bacteria-antibiotics-needed/ru/ Access date 29.10.2017].
3. The global view of Campylobacteriosis. Report of an expert consultation, (2012). Utrecht, Netherlands 9–11 July.—136 р.
4. Altekruse, S.F., Stern, N.J., Fields, P.I., Swerdlow, D.L. (1999). Campylobacter jejuni — an emerging foodborne pathogen. Emerging Infectious Disease, 5(1), 28–35.
5. Campylobacter. Fact sheet. WHO. [Electronic resource: http://www.who.int/mediacentre/factsheets/fs255/ru/. Access date 29.10.2017].
6. Microbiological Risk Assessment Series. Salmonella and Campylobacter in chicken meat. (2009). Meeting report FAO and WHO.
7. Codex Alimentarius CAC/GL 78–2011, (2011) Guidelines for the Control of Campylobacter and Salmonella in Chicken Meat.
8. Efimochkina, N.R., Bykova, I.B., Stetsenko V.V., Minaeva L.P., Pichugina T.V., Markova Yu.M., Korotkevich Yu.V., Kozak S.S., Sheveleva S.A. (2016). Study of the nature of contamination and levels of bacteria of the genus Campylobacter in certain types of food products. Voprosy pitaniya, 85(5), 52–59. (in Russian).
9. Little, C.L., Richardson, J.F., Owen, R.J., de Pinna, E., Threlfall, E.J. (2008). Campylobacter and Salmonella in raw red meats in the United Kingdom: Prevalence, characterization and antimicrobial resistance pattern, 2003–2005. Food microbiology, 25(3), 538–543.
10. Bessarabov B.F., Vashutin A.A., Voronin Ye.S. and other. Infectious diseases of animals. Moscow.: KolosS.—671 р. (in Russian).
11. Dikeman, M., Devine, C. (2014). Encyclopedia of Meat Sciences. Second Edition. Cambridge: Academic Press. — 1525 р. ISBN: 978–012384731–7;978–012384734–8
12. Bojanić, K., Midwinter, A.C. Marshall, J.C., Rogers, L.E., Biggs, P.J., Acke, E. (2017) Isolation of сampylobacter spp. From сlient-оwned dogs and cats, and retail raw meat pet food in the Manawatu, New Zealand. Zoonoses Public Health, 64(6). 438–449.
13. Groff, A.C.M., Kirinus, J.K., Sá e Silva, M., Machado, G., Costa, M.M., Vargas, A.P.C. (2010). Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis. Pesquisa Veterinária Brasileira, 30(12), 1031–1035
14. Chaban, B., Chu, S., Hendrick, S., Waldner, C., Hill, J.E. (2012). Evaluation of a Campylobacter fetus subspecies venerealis realtime quantitative polymerase chain reaction for direct analysis of bovine preputial samples. Canadian Journal of Veterinary Research, 76(3), 166–173.
15. Online Browsing Platform ISO [Electronic resource: https://www.iso.org/obp/ui/#iso: std: iso:10272:-1: ed‑2: v1: en Access date 20.10.017].
For citation:
Bataeva D.S., Minaev M.Yu., Yushina Yu.K., Sokolova O.V., Zajko E.V. CONCEPTUAL APPROACHES TO THE RAPID DETECTION OF CAMPYLOBACTER SPP. IN MEAT OF SLAUGHTER ANIMALS. Theory and practice of meat processing. 2017;2(4):76-95. https://doi.org/10.21323/2414-438X-2017-2-4-76-95
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