Enhancing functional fermented dendeng (Indonesian jerky) with Moringa oleifera: Nutritional, microbiological, and sensory aspects
https://doi.org/10.21323/2414-438X-2026-11-2-124-134
Abstract
Fermented chicken dendeng is a traditional dried meat product with improved nutritional and microbiological quality due to fermentation. This study evaluates the effects of Lactobacillus plantarum and Moringa oleifera supplementation on pH, titratable acidity (TA), bacterial count, and proximate composition. The pH values ranged from 4.09 to 4.62, with the lowest in the 6 % L. plantarum + 2.5 % M. oleifera treatment. Titratable acidity was the highest (1.47 %) in the 6 % L. plantarum group but decreased with M. oleifera addition. The highest bacterial count (6.535 log CFU/g) was observed in the 6 % L. plantarum + 5 % M. oleifera group, indicating probiotic activity. Proximate analysis shows that fermentation enhances quality. Protein content increased with M. oleifera, reaching 58.2 % in the 6 % L. plantarum + 5 % M. oleifera treatment. Fat content remained stable (8.6 % — 9.3 %), while ash content increased to 5.1 %. These findings suggest that fermented chicken dendeng with L. plantarum and optimal M. oleifera supplementation had improved microbial stability and protein retention. Further research is needed to assess storage stability and sensory attributes.
Keywords
About the Authors
D. AmertaningtyasIndonesia
Dedes Amertaningtyas, Associate Professor
Jl. Veteran Malang 65145
A. K. Umam
Indonesia
Ahmad K. Umam, Assistant Professor
Jl. Pringgodani Kediri 64111
R. Widyaningrum
Indonesia
Rhicita Widyaningrum, Student
Jl. Pringgodani Kediri 64111
D. P. P. Alina
Indonesia
Daiska P. P. Alina, Student
Jl. Pringgodani Kediri 64111
F. E. Hermanto
Indonesia
Feri E. Hermanto, Associate Professor
Jl. Veteran Malang 65145
M. H. Rawi
Malaysia
Muhamad H. Rawi, Associate Professor, Food Security Research Laboratory
Jalan UMS, 88400 Kota Kinabalu
References
1. Ayodele, P. F., Oyedotun, O., Onifade, O. F., Adeosun, A. M., Odeniyi, I. A., Omowaye, O. S. et al. (2021). Nutritional evaluation of moringa oleifera leaves and the effect of its bio-fortification with animal feed on physical changes and organ weights in male albino rats. Asian Journal of Research in Biochemistry, 9(4), 1–8. https://doi.org/10.9734/ajrb/2021/v9i430206
2. Chiș, A., Noubissi, P. A., Pop, O. -L., Mureșan, C. I., Tagne, M. A. F., Kamgang, R. et al. (2024). Bioactive compounds in Moringa oleifera: Mechanisms of action, focus on their antiinflammatory properties. Plants, 13(1), Article 20. https://doi.org/10.3390/plants13010020
3. Wang, Y., Han, J., Wang, D., Gao, F., Zhang, K., Tian, J. et al. (2022). Research update on the impact of lactic acid bacteria on the substance metabolism, flavor, and quality characteristics of fermented meat products. Foods, 11(14), Article 2090. https://doi.org/10.3390/foods11142090
4. Chen, L., Liu, R., Wu, M., Ge, Q., Yu, H. (2024). A review on aroma-active compounds derived from branched-chain amino acid in fermented meat products: Flavor contribution, formation pathways, and enhancement strategies. Trends in Food Science and Technology, 145, Article 104371. https://doi.org/10.1016/j.tifs.2024.104371
5. Syaputri, Y., Iwahashi, H. (2020). Characteristics of Heterologous Plantaricin from Lactobacillus plantarum and its future in food preservation. Reviews in Agricultural Science, 8, 124–137. https://doi.org/10.7831/ras.8.0_124
6. Mani-López, E., Hernández-Figueroa, R. H., López-Malo, A., Morales-Camacho, J. I. (2024). Viability and functional impact of probiotic and starter cultures in salami-type fermented meat products. Frontiers in Chemistry, 12, Article 1507370. https://doi.org/10.3389/fchem.2024.1507370
7. Umam, A. K., Radiati, L. E., Susila, A., Hapsari, R. N. (September 23–25, 2019). Chemical and microbiological quality of fermented goat meat dendeng with different levels of L. plantarum. IOP Conference Series: Earth and Environmental Science, 387(1), Article 012012. https://doi.org/10.1088/1755-1315/387/1/012012
8. Tamanna, N., Mahmood, N. (2015). Food processing and Maillard reaction products: Effect on human health and nutrition. International Journal of Food Science, 2015, Article 526762. https://doi.org/10.1155/2015/526762
9. Zhang, K., Huang, J., Wang, D., Wan, X., Wang, Y. (2024). Covalent polyphenols-proteins interactions in food processing: Formation mechanisms, quantification methods, bioactive effects, and applications. Frontiers in Nutrition, 11, Article 1371401. https://doi.org/10.3389/fnut.2024.1371401
10. Gobezie, E. (2021). Supplementation of moringa oleifera leaf meal in layer chickens’ feed: A Review. World’s Veterinary Journal, 11(2), 202–207. https://doi.org/10.54203/scil.2021.wvj26
11. Elabd, E. M. Y., Morsy, S. M., Elmalt, H. A. (2018). Investigating of moringa oleifera role on gut microbiota composition and inflammation associated with obesity following high fat diet feeding. Open Access Macedonian Journal of Medical Sciences, 6(8), 1359–1364. https://doi.org/10.3889/oamjms.2018.313
12. Abdallah, R., Mostafa, N. Y., Kirrella, G. A.K., Gaballah, I., Imre, K., Morar, A. et al. (2023). Antimicrobial effect of Moringa oleifera leaves extract on foodborne pathogens in ground beef. Foods, 12(4), Article 766. https://doi.org/10.3390/foods12040766
13. Hodas, F., Zorzenon, M. R. T., Milani, P. G. (2021). Moringa oleifera potential as a functional food and a natural food additive: A biochemical approach. Anais Da Academia Brasileira de Ciencias, 93(Suppl 4), Article e20210571. https://doi.org/10.1590/0001-3765202120210571
14. Kashyap, P., Kumar, S., Riar, C. S., Jindal, N., Baniwal, P., Guiné, R. P. F. et al. (2022). recent advances in drumstick (Moringa oleifera) leaves bioactive compounds: Composition, health benefits, bioaccessibility, and dietary applications. Antioxidants, 11(2), Article 402. https://doi.org/10.3390/antiox11020402
15. Jamhari, J., Suryanto, E., Sundari, S., Laksmiwati, D. A. (2018). The effect of sugar cane levels and drying methods on chemical and physical qualities of ground beef “Dendeng”. Buletin Peternakan, 42(1), 67–71. https://doi.org/10.21059/buletinpeternak.v42i1.28761
16. Chen, Y., Qiao, Y., Xiao, Y., Chen, H., Zhao, L., Huang, M. et al. (2016). Differences in physicochemical and nutritional properties of breast and thigh meat from crossbred chickens, commercial broilers, and spent hens. Asian Australasian Journal of Animal Sciences, 29(6), 855–864. https://doi.org/10.5713/ajas.15.0840
17. Zhou, T., Wu, J., Zhang, M., Ke, W., Shan, K., Zhao, D. et al. (2023). Effect of natural plant extracts on the quality of meat products: A meta-analysis. Food Materials Research, 3, Article 15. https://doi.org/10.48130/FMR-2023-0015
18. AOAC (2000). Official Methods of Analysis. The Association of Official Analytical Chemists, Gaithersburg, MD, USA, 2000.
19. Karakök, S.G., Ozogul, Y., Saler, M., Ozogul, F. (2010). Proximate analysis. Fatty acid profiles and mineral contents of meats: A comparative study. Journal of Muscle Foods, 21, 210–223. https://doi.org/10.1111/j.1745-4573.2009.00177.x
20. Setiawati, A. R., Amertaningtyas, D., Umam, A. K. (2025). Effect of adding kaffir lime leaf powder (Citrus hystrix) and citronella leaf powder (Cymbopogon nardus) in salted egg on pH, antioxidant, moisture content, and organoleptics. Jurnal Ilmu Dan Teknologi Hasil Ternak, 20(1), 61–75. https://doi.org/10.21776/ub.jitek.2025.020.01.5
21. Umam, A.K., Radiati, L.K., Suwondo, K.H.P., Kholidah, S.N. (September 21–22, 2021). Study of antioxidant activity, peptides, and chemical quality of goat milk kefir on the different postacidification periods during cold storage. 9th International Seminar on Tropical Animal Production (ISTAP 2021). Atlantis Press International B. V., 2022. https://doi.org/10.2991/absr.k.220207.037
22. Sáez, G. D., Flomenbaum, L., Zárate, G. (2018). Lactic acid bacteria from argentinean fermented foods: Isolation and characterization for their potential use as starters for fermentation of vegetables. Food Technology and Biotechnology, 56(3), 398–410. https://doi.org/10.17113/ftb.56.03.18.5631
23. Li, S., Tang, S., Mo, R., Li, J., Chen, L. (2023). Effects of NaCl curing and subsequent fermentation with Lactobacillus sakei or Lactobacillus plantarum on protein hydrolysis and oxidation in yak jerky. LWT, 173, Article 114298. https://doi.org/10.1016/j.lwt.2022.114298
24. Rezac, S., Kok, C. R., Heermann, M., Hutkins, R. (2018). Fermented foods as a dietary source of live organisms. Frontiers in Microbiology, 9, Article 1785. https://doi.org/10.3389/fmicb.2018.01785
25. Wang, D., Cheng, F., Wang, Y., Han, J., Gao, F., Tian, J. et al. (2022). The changes occurring in proteins during processing and storage of fermented meat products and their regulation by lactic acid bacteria. Foods, 11(16), Article 2427. https://doi.org/10.3390/foods11162427
26. Renitasari, F., Masini, Ayuningtyas. (2023). The Effect of supplementary food in the form of moringa leaf dry brownies on the increasing weight of under-fives with underweight status in Wonokerto Village Turi Sleman. Formosa Journal of Science and Technology, 2(3), 735–750. https://doi.org/10.55927/fjst.v2i3.3333
27. Nunes, L., Martins, E., Perrone, Í.T., de Carvalho, A.F. (2019). The Maillard reaction in powdered infant formula. Journal of Food and Nutrition Research, 7(1), 33–40. https://doi.org/10.12691/jfnr-7-1-5
28. Fu, Y., Zhang, Y., Soladoye, O. P., Aluko, R. E. (2020). Maillard reaction products derived from food protein-derived peptides: Insights into flavor and bioactivity. Critical Reviews in Food Science and Nutrition, 60(20), 3429–3442. https://doi.org/10.1080/10408398.2019.1691500
29. Lund, M. N., Ray, C. A. (2017). Control of Maillard reactions in foods: Strategies and chemical mechanisms. Journal of Agricultural and Food Chemistry, 65(23), 4537–4552. https://doi.org/10.1021/acs.jafc.7b00882
30. Peñalver, R., Martínez‐zamora, L., Lorenzo, J. M., Ros, G., Nieto, G. (2022). Nutritional and antioxidant properties of Moringa oleifera leaves in functional foods. Foods, 11(8), Article 1108. https://doi.org/10.3390/foods11081107
31. Pongsetkul, J., Benjakul, S., Vongkamjan, K., Sumpavapol, P., Osako, K. (2017). Microbiological and chemical changes of shrimp Acetes vulgaris during Kapi production. Journal of Food Science and Technology, 54(11), 3473–3482. https://doi.org/10.1007/s13197-017-2804-4
32. Ma, Y., Fei, Y., Han, X., Liu, G., Fang, J. (2022). Lactobacillus plantarum alleviates obesity by altering the composition of the gut microbiota in high-fat diet-fed mice. Frontiers in Nutrition, 9, Article 947367. https://doi.org/10.3389/fnut.2022.947367
33. Agrawal, N., Singh, P. K., Jairath, G., Ahmad, M. F., Raposo, A., Khanam, A. et al. (2024). Physico-chemical changes in developed probiotic chicken meat spread fermented with Lactobacillus acidophilus and malted millet flour. Applied Food Research, 4(2), Article 100484. https://doi.org/10.1016/j.afres.2024.100484
34. Özer, C. O., Kılıç, B. (2021). Optimization of pH, time, temperature, variety and concentration of the added fatty acid and the initial count of added lactic acid Bacteria strains to improve microbial conjugated linoleic acid production in fermented ground beef. Meat Science, 171, Article 108303. https://doi.org/10.1016/j.meatsci.2020.108303
35. Ichikawa, N., Ng, L. S., Makino, S., Goh, L. L., Lim, Y. J., Ferdinandus et al. (2022). solid-state fermented okara with Aspergillus spp. improves lipid metabolism and high-fat diet induced obesity. Metabolites, 12(3), Article 198. https://doi.org/10.3390/metabo12030198
36. Carneiro, K. O., Campos, G. Z., Lima, J. M.S., da Silva Rocha, R., Vaz-Velho, M., Todorov, S. D. (2024). The role of lactic acid bacteria in meat products, not just as starter cultures. Foods, 13(19), Article 3170. https://doi.org/10.3390/foods13193170
37. Umam, A.K., Lin, M.-J., Radiati, L. E., Peng, S.-Y. (2018). The capability of Canna edulis ker starch as carboxymethyl cellulose replacement on yogurt drink during cold storage. Animal Production, 20(2), 109–118.
38. Sharma, R., Garg, P., Kumar, P., Bhatia, S. K., Kulshrestha, S. (2020). Microbial fermentation and its role in quality improvement of fermented foods. Fermentation, 6(4), Article 106. https://doi.org/10.3390/fermentation6040106
39. Radiati, L. E., Umam, A. K., Susilo, A., Thoifi, A. A. (October 24– 27, 2019). Effect of Lactobacillus plantarum concentration level on physicochemical properties of fermented goat meat dendeng. IOP Conference Series: Earth and Environmental Science, 478(1), Article 012038. https://doi.org/10.1088/1755-1315/478/1/012038
40. Radiati, L. E., Damayanthi, E., Armaini, A., Santoso, A., Hati, D. L., Fardiaz, D. et al. (2025). Exopolysaccharide of goat milk kefir as an anti-obesity agent: Inhibition of adipogenesis enzyme activity on 3T3-L1 adipocyte model cells. Journal of Advanced Pharmaceutical Technology and Research, 16(2), 47–52. https://doi.org/10.4103/JAPTR.JAPTR_169_24
41. Tang, H., Huang, W., Yao, Y. -F. (2023). The metabolites of lactic acid bacteria: Classification, biosynthesis and modulation of gut microbiota. Microbial Cell, 10(3), 49–62. https://doi.org/10.15698/mic2023.03.792
42. Chen, X., Pan, S., Li, F., Xu, X., Xing, H. (2022). Plant-derived bioactive compounds and potential health benefits: Involvement of the gut microbiota and its metabolic activity. Biomolecules, 12(12), Article 1871. https://doi.org/10.3390/biom12121871
43. Cai, Z., Ruan, Y., He, J., Dang, Y., Cao, J., Sun, Y. et al. (2020). Effects of microbial fermentation on the flavor of cured duck legs. Poultry Science, 99(9), 4642–4652. https://doi.org/10.1016/j.psj.2020.06.019
44. Munekata, P. E. S., Pateiro, M., Tomasevic, I., Domínguez, R., da Silva Barretto, A. C., Santos, E. M. et al. (2022). Functional fermented meat products with probiotics — A review. Journal of Applied Microbiology, 133(1), 91–103. https://doi.org/10.1111/jam.15337
Review
For citations:
Amertaningtyas D., Umam A.K., Widyaningrum R., Alina D.P., Hermanto F.E., Rawi M.H. Enhancing functional fermented dendeng (Indonesian jerky) with Moringa oleifera: Nutritional, microbiological, and sensory aspects. Theory and practice of meat processing. 2026;11(2):124-134. https://doi.org/10.21323/2414-438X-2026-11-2-124-134
JATS XML





































