Preview

Theory and practice of meat processing

Advanced search

Effect of the moringa leaves extract on the tenderization of Longissimus muscle in Indonesian native goats

https://doi.org/10.21323/2414-438X-2026-11-1-35-44

Abstract

Meat tenderization techniques often involve time-consuming processes or chemical additives that may raise health concerns or alter the taste of meat. Exploring alternative natural methods, such as utilizing proteolytic enzymes derived from moringa, presents an opportunity to address these limitations. Studies on moringa have been limited, especially those focusing on goat meat tenderization, leaving a gap in understanding how moringa affects this process. The research aims to bridge this gap by systematically examining how protease enzymes from moringa leaves can tenderize goat meat. The research was performed on goat meat samples (100 g of meat per sample) treated with the moringa leaves extract (prepared from 20 g of moringa leaves with 60 ml of distilled water) at different storage times (2, 24, and 46 hours), as well as a control sample without any additive. The results showed that the moringa leaves extract did not significantly alter pH values and increased cooking loss for the 24-hour treatment (44.3 %) and 46-hour treatment (39.8 %) compared to the control (36.8 %). Color analysis showed increased lightness (L*) and redness (a*). The yellowness (b*) showed considerable difference between the control and the 24-hour samples compared with the 2-hour and 46-hour samples. The moringa leaves extract significantly reduced tenderness by lowering hardness, gumminess, resilience, and chewiness. This research advances environmentally friendly, renewable solutions in the food industry because the food industry produces significant quantities of meat by-products and waste during processing. This research will reduce waste by transforming tougher or less desirable meat cuts into more tender and valuable products.

About the Authors

T. H. A. Mohamed
Omdurman Islamic University Jenderal Soedirman University
Sudan

Tasnim H. A. Mohamed, Ph.D., Lecturer, Department of Animal Production, Faculty of Agriculture

P. O. Box 382, Omdurman, Sudan



J. Sumarmono
Jenderal Soedirman University
Indonesia

Juni Sumarmono, Professor in Post-harvest Processing Technology, Faculty of Animal Science 

Soeparno Street, 60, Purwokerto, Central Java, Indonesia



A. Fatoni
Jenderal Soedirman University
Indonesia

Amin Fatoni, Professor in Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences 

Soeparno Street, 60, Purwokerto, Central Java, Indonesia



T. Setyawardani
Jenderal Soedirman University
Indonesia

Triana Setyawardani, Professor in Animal Products Processing Technology, Faculty of Animal Science 

Soeparno Street, 60, Purwokerto, Central Java, Indonesia



S. Rahayu
Jenderal Soedirman University
Indonesia

Sri Rahayu, Professor in Animal Biotechnology, Faculty of Animal Science 

Soeparno Street, 60, Purwokerto, Central Java, Indonesia



A. Setyaningrum
Jenderal Soedirman University
Indonesia

Agustinah Setyaningrum, Associate Professor in Animal Production, Faculty of Animal Science 

Soeparno Street, 60, Purwokerto, Central Java, Indonesia



A. Frah
Omdurman Islamic University
Sudan

Amani Z. A. Frah, Ph.D., Assistant Professor in Animal Genetics, Department of Animal Production, Faculty of Agriculture

P. O. Box 382, Omdurman, Sudan



References

1. Bekhit A.E-DA. (2017). Advances in Meat Processing Technology. CRC Press Taylor & Francis. https://doi.org/10.1201/9781315371955

2. Amin, T., Bhat, S., Sharma, N. (2014). Technological advancements in meat tenderization: A review. Journal of Meat Science and Technology, 2(1), 1–9.

3. Akhlaghi, N., Najafpour-Darzi, G. (2023). Potential applications of ginger rhizomes as a green biomaterial: A review. International Journal of Engineering: Basics, Applications and Aspect, 36(2), 372–383. https://doi.org/10.5829/ije.2023.36.02b.16

4. Sorapukdee, S., Samritphol, W., Sangsawad, P., Tangwatcharin, P. (2024). Application of Collagenolytic proteases from Bacillus subtilis B13 and Bacillus siamensis S6 for tenderizing goat meat during wet aging. Food Science of Animal Resources, 44(2), 430–442. https://doi.org/10.5851/kosfa.2024.e79

5. Issa-Zacharia, A., Muhimbula, H.S., Bikuba, A.N., Matondo, J.D. (2024). Development of natural liquid and powdered meat tenderizer based on papaya peel, ginger and garlic. Journal of Food and Nutrition Sciences, 12(2), 107–118. https://doi.org/10.11648/j.jfns.20241202.13

6. Balakireva, A.V., Kuznetsova, N.V., Petushkova, A.I., Savvateeva, L. V., Zamyatnin, (Jr.) A.A. (2019). Trends and prospects of plant proteases in therapeutics. Current Medicinal Chemistry, 26(3), 465–486. https://doi.org/10.2174/0929867325666171123204403

7. Klimek-Szczykutowicz, M., Gawet-Beben, K., Rutka, A., Blicharska, E., Tatarczak-Michalewska, M., Kulik-Siarek, K. et al. (2024). Moringa oleifera (drumstick tree) — nutraceutical, cosmetological and medicinal importance: A review. Frontiers in Pharmacology, 15, Article 1288382. https://doi.org/10.3389/fphar.2024.1288382

8. Arawande, J.O., Adeleke, A.R., Orimoloye, O.R., Adebisi, S.A., Amuho, E.U., Ijitona, O.O. (2021). Extractive values and antioxidant properties of leaf, seeds, pods and coats moringa plant. Biomedical Journal of Scientific and Technical Research, 39(3), 1530–31536. https://doi.org/10.26717/bjstr.2021.39.006334

9. Abdallah, R., Mostafa, N.Y., Kirrella, G.A.K., Gaballah, I., Imre, K., Morar, A. et al. (2023). Antimicrobial effect of Moringa oleifera leaves extract on foodborne pathogens in ground beef. Foods, 12(4), Article 766. https://doi.org/10.3390/foods12040766

10. Banik, S., Biswas, S., Karmakar, S. (2018). Extraction, purification, and activity of protease from the leaf of Moringa oleifera. F1000Research, 7, Article 1151. https://doi.org/10.12688/f1000research.15642.1

11. Sandeep, G., Arumugam, T., Janavi, G.J., Anitha, T., Senthil, K., Lakshmanan, A. (2023). A comparative study on conventional and non-conventional extraction methodologies for extraction yield, quality and antibacterial properties of moringa (Moringa oleifera Lam.). Journal of Applied Horticulture, 25(1), 17–24. https://doi.org/10.37855/jah.2023.v25i01.03

12. Mashau, M.E., Munandi, M., Ramashia, S.E. (2021). Exploring the influence of Moringa oleifera leaves extract on the nutritional properties and shelf life of mutton patties during refrigerated storage. CyTA — Journal of Food, 19(1), 389–398. https://doi.org/10.1080/19476337.2021.1910732

13. Mohamed, T.H.A., Sumarmono, J., Fatoni, A., Frah, A.Z.A., Fadhlurrohman I., Yusuf H. M. (September 26–27, 2024). Improving Goat Meat (Longissimus Muscle) Tenderization by Using Moringa Oleifera Leaf. The 7th International Conference on Multidiscipline Approaches for Sustainable Rural Development (ICMA SURE 2024). Java Heritage, Purwokerto, Indonesia, 2024. https://doi.org/10.1051/e3sconf/202560902007

14. Kartikasari, L.R., Hertanto, B.S., Barido, F.H., Swastike, W., Nuhriawangsa, A.M.P. (2023). The effect of Moringa oleifera L. leaf extract supplementation on the chemical and sensory quality of beef patties cooked to a well-done level. Jurnal Ilmu-Ilmu Peternakan, 33(3), 457–468. https://doi.org/10.21776/ub.jiip.2023.033.03.15

15. Ardite, I.G.N., Sriyani, N.L.P., Suarta, G. (2023). Organoleptic value of Bali beef marinated with fresh moringa leaf, moringa leaf tea, and moringa leaf powder. Majalah Ilmiah Peternakan, 26(2), 110–115. https://doi.org/10.24843/MIP.2023. v26.i02.p07

16. Lungu, N.S., Idamokoro, E.M. (2024). Bio-functional prospects of Moringa oleifera lam on meat quality — A review. American Journal of Animal and Veterinary Sciences, 19(2), 150–166. https://doi.org/10.3844/ajavsp.2024.150.166

17. Kenawi, M. A., El-Hameed, S. M. A. (2018). Effect of Moringa Oleifera Leaf extract on the quality of buffalo meat product. Scientific Papers-Animal Science Series: Lucrări Ştiinţifice — Seria Zootehnie, 70, 202–207.

18. Das, A.K., Rajkumar, V, Verma, A.K., Swarup, D. (2012). Moringa oleiferia leaf extract: A natural antioxidant for retarding lipid peroxidation in cooked goat meat patties. International Journal of Food Science and Technology, 47, 585–591. https://doi.org/10.1111/j.1365-2621.2011.02881.x

19. Akib, M.G., Rifat, A., Bormon, C., Dutta, A., Ataher, M.S., Azzam, M. et al. (2024). Effects of Moringa oleifera leaf powder on the growth performance, meat quality, blood parameters, and cecal bacteria of broilers. Veterinary Sciences, 11(8), Article 374. https://doi.org/10.3390/vetsci11080374

20. Mwankunda, L.J., Nyamete, F., Kilima, B. (2023). Exploring the influence of Moringa oleifera leaves extract on the shelf life of ground beef during refrigerated storage. European Journal of Medicinal Plants, 34(10), 42–52. https://doi.org/10.9734/ejmp/2023/v34i101164

21. Abubakar, F.B., Abdulsalam, A.Y., Abdul-Azeez, H., Suleiman, A.M., Muhd, I.U., Mudi, Z.R. et al. (2024). Effect of moringa (Moringa oleifera) leav and ginger (Zingiber offi nale) rhizome marinades on the proximate and sensory qualities of smoked African catfish (Clarias gariepinus, Burchell 1822). International Journal of Fisheries and Aquatic Studies, 12(3, Part A), 47–50. https://doi.org/10.22271/fish.2024.v12.i3a.2933

22. Mickdam, E., Alwaleed, S., Madany, M., Sayed, A. (2022). The effect of Moringa oleifera leaves on chicken meat composition and meat quality. International Journal of Veterinary Science, 11(2), 201–206. https://doi.org/10.47278/journal.ijvs/2021.094

23. Apriantini, A., Putra, R.G., Suryati, T. (2022). A review: Application of Moringa oleifera extract in various processed meat products. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 10(3), 132–143. https://doi.org/10.29244/jipthp.10.3.132–143 (In Indonesian)

24. Honikel, K.O. (1998). Reference methods for the assessment of physical characteristics of meat. Meat Science, 49(4), 447– 457. https://doi.org/10.1016/S0309-1740(98)00034-5

25. Nahdhiyati, A., Krisna, A. (2014). Extraction and characterization of protease enzyme from moringa leaves (Moringa oliefera Lamk.). Journal Teknologi Pertanian, 15(3), 191–200. (In Indonesian)

26. Abd-Elkhalek, A.M., Seoudi, D.M., Ibrahim, O.A., AbdRabou, N.S., Abd Elazeem, E.M. (2020). Extraction, partial purification, characteristics, and antimicrobial activity of plant protease from Moringa oleifera leaves. Journal of Applied Biotechnology Reports, 7(4), 243–250. https://doi.org/10.30491/jabr.2020.230789.1225

27. Ogah, J.O., Osundare, F.A. (2015). Evaluation of antibacterial activity and preliminary phytochemical screening of Moringa oleifera on pathogenic bacteria. International Journal of Pharmacological Research, 5(11), 310–315. https://doi.org/10.7439/ijpr

28. Arbab, I.A., Abdlrazig, S.E.H., Adam, D.Y., Holy, A.S.I., Bakr, M.A., Ali, F.E.A.A. et al. (2022). Phytochemical screening and evaluation of antibacterial and antifungal activity of the ethanolic extracts of Moringa oleifera Lam leaves and Hyphaene thebaica (Doum) fruit grown in Sudan. International Journal of Advanced Multidisciplinary Research, 9(11) 54–68. http://doi.org/10.22192/ijamr.2022.09.11.007

29. Paikra, B.K, Dhongade, H.K.J., Gidwani, B. (2017). Phytochemistry and pharmacology of Moringa oleifera Lam. Journal of Pharmacopuncture, 20(3), 194–200. https://doi.org/10.3831/KPI.2017.20.022

30. Kadhim. E.J., Al-Shammaa, D.A. (2014). Phytochemical characterization using GC–MS analysis of methanolic extract of Moringa oleifera (Family Moringaceae) plant cultivated in Iraq. Chemistry and Materials Research. 6(5), 9–26.

31. Naco, M.E.B., Coulibaly, D.B., Tirera, H., Mariko, M., Koumare, B.Y., Ndiaye, B. et al. (2024). Study of the phytochemical composition and antioxidant activities of Moringa oleifera leaves. Lam (Moringaceae) in the regions of Kita (Mali) and Th (Senegal). International Journal of Biological and Chemical Sciences, 18(4), 1554–1568. https://doi.org/10.4314/ijbcs.v18i4.26

32. Imohiosen, O., Gurama, H.H., Lamidi, T.B. (2014). Phytochemical and antimicrobial studies on Moringa Oleifera leaves extracts. IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT), 8(1), 39–45. https://doi.org/10.9790/2402–08143945

33. Anzano, A., de Falco, B., Ammar, M., Ricciardelli, A., Grauso, L., Sabbah, M. et al. (2022). Chemical analysis and antimicrobial activity of Moringa oleifera Lam. leaves and seeds. Molecules, 27(4), Article 8920. https://doi.org/10.3390/molecules27248920

34. Botinestean, C., Keenan, D.F., Kerry, J.P., Hamill, R.M. (2016). The effect of thermal treatments, including sous-vide, blast freezing, and their combinations, on beef tenderness of M. semitendinosus steaks targeted at elderly consumers. LWT, 74, 154–159. http://doi.org/10.1016/j.lwt.2016.07.026

35. Rahman, M.H., Alam, M.S., Monir, M.M., Rahman, S.M.E. (2020). Effect of Moringa oleifera leaf extract and synthetic antioxidant on quality and shelf-life of goat meat nuggets at frozen storage. International Journal of Food Research, 7(3), 34–45. https://doi.org/10.33500/ijfr.2020.07.004

36. Rizqiati, H., Nugraheni, A., Susanti, S., Fatmawati, L., Nuryanto, N., Arifan, F. (2021). Characteristic of isolated crude bromelain extract from cayenne pineapple crown in various drying temperature and its effect on meat texture. Food Research, 5(5), 72–79. https://doi.org/10.26656/fr.2017.5(5).692

37. Kakash, S.B., Hojjatoleslamy, M., Babaei, G., Molavi. H. (2019). Kinetic study of the effect of kiwi fruit actinidin on various proteins of chicken meat. Food Science and Technology, 39(4), 980–992. https://doi.org/10.1590/fst.14118

38. Murtini, E., dan Qomarudin. (2003). Meat tenderization using protease of “Biduri” plant (Calotropis gigantea). Jurnal Teknologi dan Industri Pangan, XIV(3), 266–268. (In Indonesian)

39. Gagaoua, M., Dib, A.L., Lakhdara, N., Lamri, M., Botineştean, C., Lorenzo, J.M. (2021). Artificial meat tenderization using plant cysteine proteases. Current Opinion in Food Science, 38, 177–188. https://doi.org/10.1016/j.cofs.2020.12.002

40. Azmi, S.I.M., Kumar, P., Sharma, N., Sazili, A.Q., Lee, S.J., Ismail-Fitry, M.R. et al. (2023). Application of plant proteases in meat tenderization: Recent trends and future prospects. Foods, 12(6), Article 1336. https://doi.org/10.3390/foods12061336

41. Bekhit, A.E.-D.A., Hopkins, D.L., Geesink, G., Bekhit, A.A., Franks, P. (2014). Exogenous proteases for meat tenderization. Critical Reviews in Food Science and Nutrition, 54(8), 1012–1031. https://doi.org/10.1080/10408398.2011.623247

42. Moyo, B., Masika, P., Muchenje, V. (2014). Effect of feeding Moringa (Moringa oleifera) leaf meal on the physicochemical characteristics and sensory properties of goat meat. South African Journal of Animal Science, 44(1), 64–70. https://doi.org/10.4314/sajas.v44i1.9

43. Sulaiman, A., Wibowo, A., Suhardi, Aprylasari, D., Haris, M.I., Nur Wahyuningtyas, A. et al. (2025). Optimizing Citrus aurantifolia juice marination for improved chicken meat quality: A study on pH, tenderness, water retention, and cooking loss. Jurnal Ilmu dan Teknologi Hasil Ternak, 20(2), 130–142. https://doi.org/10.21776/ub.jitek.2025.020.02.4

44. Ruedt, C., Gibis, M., Weiss, J., (2023). Meat color and iridescence: Origin, analysis, and approaches to modulation. Comprehensive Reviews in Food Science and Food Safety, 22(4), 3366–3394. https://doi.org/10.1111/1541-4337.13191

45. Feihrmann, A.C., Nascimento, M.G., Belluco, C.Z., Fioroto, P.O., Cardozo-Filho, L., Tonon, L.C. (2017). Evaluation of the effect of antioxidant Moringa oleifera extract in beef. Chemical Engineering Transactions, 57, 1993–1998. https://doi.org/10.3303/CET1757333

46. Gao, H.Y., Zhou, G.H., Zeng, J., Ma, H.J., Pan, R.S., Yu. X.L. (2011). Tenderization of goose meat by papain, pineapple juice and ginger juice treatment. Advanced Materials Research, 236–238, 2349–2352. https://doi.org/10.4028/www.scientific.net/AMR.236-238.2349


Review

For citations:


Mohamed T.H., Sumarmono J., Fatoni A., Setyawardani T., Rahayu S., Setyaningrum A., Frah A. Effect of the moringa leaves extract on the tenderization of Longissimus muscle in Indonesian native goats. Theory and practice of meat processing. 2026;11(1):35-44. https://doi.org/10.21323/2414-438X-2026-11-1-35-44

Views: 635

JATS XML


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2414-438X (Print)
ISSN 2414-441X (Online)