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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">meat</journal-id><journal-title-group><journal-title xml:lang="en">Theory and practice of meat processing</journal-title><trans-title-group xml:lang="ru"><trans-title>Теория и практика переработки мяса</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2414-438X</issn><issn pub-type="epub">2414-441X</issn><publisher><publisher-name>ФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2414-438X-2026-11-1-35-44</article-id><article-id custom-type="elpub" pub-id-type="custom">meat-551</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Effect of the moringa leaves extract on the tenderization of Longissimus muscle in Indonesian native goats</article-title><trans-title-group xml:lang="ru"><trans-title>Effect of the moringa leaves extract on the tenderization of Longissimus muscle in Indonesian native goats</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0006-5989-0425</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Mohamed</surname><given-names>T. H. A.</given-names></name><name name-style="western" xml:lang="en"><surname>Mohamed</surname><given-names>T. H. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Tasnim H. A. Mohamed, Ph.D., Lecturer, Department of Animal Production, Faculty of Agriculture</p><p>P. O. Box 382, Omdurman, Sudan</p></bio><bio xml:lang="en"><p>Tasnim H. A. Mohamed, Ph.D., Lecturer, Department of Animal Production, Faculty of Agriculture</p><p>P. O. Box 382, Omdurman, Sudan</p></bio><email xlink:type="simple">tasnimmohamed806@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8269-4189</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Sumarmono</surname><given-names>J.</given-names></name><name name-style="western" xml:lang="en"><surname>Sumarmono</surname><given-names>J.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Juni Sumarmono, Professor in Post-harvest Processing Technology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><bio xml:lang="en"><p>Juni Sumarmono, Professor in Post-harvest Processing Technology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><email xlink:type="simple">juni.sumarmono@unsoed.ac.id</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6550-2461</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Fatoni</surname><given-names>A.</given-names></name><name name-style="western" xml:lang="en"><surname>Fatoni</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Amin Fatoni, Professor in Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><bio xml:lang="en"><p>Amin Fatoni, Professor in Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><email xlink:type="simple">amin.fatoni@unsoed.ac.id</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9017-2513</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Setyawardani</surname><given-names>T.</given-names></name><name name-style="western" xml:lang="en"><surname>Setyawardani</surname><given-names>T.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Triana Setyawardani, Professor in Animal Products Processing Technology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><bio xml:lang="en"><p>Triana Setyawardani, Professor in Animal Products Processing Technology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><email xlink:type="simple">triana.setyawardani@unsoed.ac.id</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5063-6846</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Rahayu</surname><given-names>S.</given-names></name><name name-style="western" xml:lang="en"><surname>Rahayu</surname><given-names>S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Sri Rahayu, Professor in Animal Biotechnology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><bio xml:lang="en"><p>Sri Rahayu, Professor in Animal Biotechnology, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><email xlink:type="simple">sri.rahayu2710@unsoed.ac.id</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-9878-1970</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Setyaningrum</surname><given-names>А.</given-names></name><name name-style="western" xml:lang="en"><surname>Setyaningrum</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Agustinah Setyaningrum, Associate Professor in Animal Production, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><bio xml:lang="en"><p>Agustinah Setyaningrum, Associate Professor in Animal Production, Faculty of Animal Science </p><p>Soeparno Street, 60, Purwokerto, Central Java, Indonesia</p></bio><email xlink:type="simple">agustinah.setyaningrum@unsoed.ac.id</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2444-3717</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Frah</surname><given-names>А. Z. A.</given-names></name><name name-style="western" xml:lang="en"><surname>Frah</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Amani Z. A. Frah, Ph.D., Assistant Professor in Animal Genetics, Department of Animal Production, Faculty of Agriculture</p><p>P. O. Box 382, Omdurman, Sudan</p></bio><bio xml:lang="en"><p>Amani Z. A. Frah, Ph.D., Assistant Professor in Animal Genetics, Department of Animal Production, Faculty of Agriculture</p><p>P. O. Box 382, Omdurman, Sudan</p></bio><email xlink:type="simple">amanizein@oiu.edu.sd</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Omdurman Islamic University; &#13;
Jenderal Soedirman University</institution><country>Судан</country></aff><aff xml:lang="en"><institution>Omdurman Islamic University&#13;
Jenderal Soedirman University</institution><country>Sudan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Jenderal Soedirman University</institution><country>Индонезия</country></aff><aff xml:lang="en"><institution>Jenderal Soedirman University</institution><country>Indonesia</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Omdurman Islamic University</institution><country>Судан</country></aff><aff xml:lang="en"><institution>Omdurman Islamic University</institution><country>Sudan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>04</day><month>04</month><year>2026</year></pub-date><volume>11</volume><issue>1</issue><fpage>35</fpage><lpage>44</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Mohamed T.H., Sumarmono J., Fatoni A., Setyawardani T., Rahayu S., Setyaningrum A., Frah A., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Mohamed T.H., Sumarmono J., Fatoni A., Setyawardani T., Rahayu S., Setyaningrum А., Frah А.Z.</copyright-holder><copyright-holder xml:lang="en">Mohamed T.H., Sumarmono J., Fatoni A., Setyawardani T., Rahayu S., Setyaningrum A., Frah A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.meatjournal.ru/jour/article/view/551">https://www.meatjournal.ru/jour/article/view/551</self-uri><abstract><p>Meat tenderization techniques often involve time-consuming processes or chemical additives that may raise health concerns or alter the taste of meat. Exploring alternative natural methods, such as utilizing proteolytic enzymes derived from moringa, presents an opportunity to address these limitations. Studies on moringa have been limited, especially those focusing on goat meat tenderization, leaving a gap in understanding how moringa affects this process. The research aims to bridge this gap by systematically examining how protease enzymes from moringa leaves can tenderize goat meat. The research was performed on goat meat samples (100 g of meat per sample) treated with the moringa leaves extract (prepared from 20 g of moringa leaves with 60 ml of distilled water) at different storage times (2, 24, and 46 hours), as well as a control sample without any additive. The results showed that the moringa leaves extract did not significantly alter pH values and increased cooking loss for the 24-hour treatment (44.3 %) and 46-hour treatment (39.8 %) compared to the control (36.8 %). Color analysis showed increased lightness (L*) and redness (a*). The yellowness (b*) showed considerable difference between the control and the 24-hour samples compared with the 2-hour and 46-hour samples. The moringa leaves extract significantly reduced tenderness by lowering hardness, gumminess, resilience, and chewiness. This research advances environmentally friendly, renewable solutions in the food industry because the food industry produces significant quantities of meat by-products and waste during processing. This research will reduce waste by transforming tougher or less desirable meat cuts into more tender and valuable products.</p></abstract><trans-abstract xml:lang="ru"><p>Meat tenderization techniques often involve time-consuming processes or chemical additives that may raise health concerns or alter the taste of meat. Exploring alternative natural methods, such as utilizing proteolytic enzymes derived from moringa, presents an opportunity to address these limitations. Studies on moringa have been limited, especially those focusing on goat meat tenderization, leaving a gap in understanding how moringa affects this process. The research aims to bridge this gap by systematically examining how protease enzymes from moringa leaves can tenderize goat meat. The research was performed on goat meat samples (100 g of meat per sample) treated with the moringa leaves extract (prepared from 20 g of moringa leaves with 60 ml of distilled water) at different storage times (2, 24, and 46 hours), as well as a control sample without any additive. The results showed that the moringa leaves extract did not significantly alter pH values and increased cooking loss for the 24-hour treatment (44.3 %) and 46-hour treatment (39.8 %) compared to the control (36.8 %). Color analysis showed increased lightness (L*) and redness (a*). The yellowness (b*) showed considerable difference between the control and the 24-hour samples compared with the 2-hour and 46-hour samples. The moringa leaves extract significantly reduced tenderness by lowering hardness, gumminess, resilience, and chewiness. This research advances environmentally friendly, renewable solutions in the food industry because the food industry produces significant quantities of meat by-products and waste during processing. This research will reduce waste by transforming tougher or less desirable meat cuts into more tender and valuable products.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Moringa leaves extract</kwd><kwd>goat meat</kwd><kwd>cooking loss</kwd><kwd>proteolytic enzymes</kwd><kwd>tenderness</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Moringa leaves extract</kwd><kwd>goat meat</kwd><kwd>cooking loss</kwd><kwd>proteolytic enzymes</kwd><kwd>tenderness</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">This research was supported by Jenderal Soedirman University through Professorship Research Grant No. 14.593/UN23.3/PT.01/V/2025 (May 14, 2025).</funding-statement><funding-statement xml:lang="en">This research was supported by Jenderal Soedirman University through Professorship Research Grant No. 14.593/UN23.3/PT.01/V/2025 (May 14, 2025).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Bekhit A.E-DA. 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