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Study of the functional product’s protein compounds digestion features

https://doi.org/10.21323/2414-438X-2020-5-3-18-21

Abstract

The aim of the study was to investigate the transformation of meat product’s proteins from pig hearts and aortas during enzymatic hydrolysis in an in vitro model of the gastrointestinal tract. The model consisted of three phases simulating digestion processes: “oral cavity” phase (a-amylase, pH 7.0; 2 min), “stomach” phase (pork pepsin, pH 3.0; 120 min), “intestine” phase (pork pancreatin, pH 7.0; 130 min). The product was sequentially subjected to hydrolysis, at the end of each phase, samples were taken to determine the protein concentration (biuret method) and visualize the protein fractions (one-dimensional electrophoresis). A significant increase in protein concentration at the “stomach” phase was revealed by 3.2 times, and the absolute content by 4.6 times. At the “intestine” phase, a decrease in the number of peptide complexes with copper ions by 1.8 times, the absolute protein content by 8.5% was re‑ vealed. The noted tendency was confirmed by electrophoretic studies — at the stage, simulating digestion in the stomach, the prod‑ ucts of meat product’s proteins hydrolysis were visualized; at the “intestine” phase, a low expression of protein fractions in the range of more than 10 kDa is shown. The maximum hydrolysis of protein compounds at the “stomach” phase to poly- and oligopeptides was confirmed, continuing at the “intestine” stage with the accumulation of free amino acids. This methodology makes it possible to visualize the products of hydrolysis of proteins in a meat product at all stages of the model and to monitor changes in protein concentration in the system.

About the Authors

E. R. Vasilevskaya
V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation

Ekaterina R.  Vasilevskaya — candidate of technical sciences, researcher, Experimental clinic-laboratory of biologically active substances of animal origin

109316, Moscow, Talalikhina str., 26. Tel.: +7–495–676–92–11



A. G. Akhremko
V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation

Anastasiya G. Akhremko — junior researcher, Experimental clinic-laboratory of biologically active substances of animal origin

109316, Moscow, Talalikhina str., 26. Tel: +7–495–676–92–11



E. K. Polishchuk
V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation

Ekaterina K.  Polishchuk — senior laboratory assistant, Experimental clinic-laboratory of biologically active substances of animal origin

109316, Moscow, Talalikhina str., 26. Tel.: +7–495– 676–92–11



L. V. Fedulova
V. M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences
Russian Federation

Liliya V. Fedulova — candidate of technical sciences, Head of Experimental clinic-laboratory of biologically active substances of animal origin

109316, Moscow, Talalikhina str., 26. Tel.: +7–495– 676–92–11



References

1. Bechaux, J., Gatellier, P., Le Page, J.F., Drillet, Y., SanteLhoutellier, V. (2019). A comprehensive review of bioactive peptides obtained from animal byproducts and their applications. Food and Function, 10(10), 6244–6266. https://doi.org/10.1039/c9fo01546a

2. Albenzio, M., Santillo, A., Caroprese, M., Della Malva, A., Marino, R. (2017). Bioactive Peptides in Animal Food Products. Foods, 6(5), 35. https://doi.org/10.3390/foods6050035

3. Kupaeva, N.V, Kotenkova, Е.А. (2019). Analysis of the antioxidant capacity of farm animal raw materials. Vsyo o myase, 5, 34–37. https://doi.org/10.21323/2071–2499–2019–5–34–37 (in Russuan)

4. Chernukha, I.M., Afanasyev, D.A., Mashentseva, N.G., Vostrikova, N.L. (2019). Biologically active peptides as a product of microbial fermentation of raw meat and finished meat prod‑ ucts: review. Part 1. General information about biologically active peptides of meat and meat products. Theory and practice of meat processing, 4(4), 12–16. https://doi.org/10.21323/2414–438X‑2019–4–4–12–16 (in Russian)

5. Chernukha, I.M., Afanasyev, D.A., Mashentseva, N.G., Vostrikova, N.L. (2020). Biologically active peptides as a product of microbial fermentation of raw meat and finished meat products: review. Part 2. Functionality of bioactive meat peptides. Theory and practice of meat processing, 5(2), 12–19. https://doi.org/10.21323/2414–438X‑2020–5–2–12–19 (in Russian)

6. Fu, Y., Zhang, Y., Soladoye, O.P., Aluko, R.E. (2019). Maillard reaction products derived from food protein-derived peptides: insights into flavor and bioactivity. Critical reviews in food science and nutrition, 18, 1–14. https://doi.org/10.1080/10408398.2019.1691500

7. Lafarga, T., O’Connor, P., Hayes, M. (2015). In silico methods to identify meat-derived prolyl endopeptidase inhibitors. Food Chemistry, 175, 337–343. https://doi.org/10.1016/j.foodchem.2014.11.150

8. Kong, F., Singh, R.P. (2010). A human gastric simulator (HGS) to study food digestion in human stomach. Journal of Food Science, 75(9), E627-E635. doi:10.1111/j.1750–3841.2010.01856.x

9. Picariello, G., Miralles, B., Mamone, G., Sánchez-Rivera, L., Recio, I., Addeo, F., Ferranti, P. (2015). Role of intestinal brush border peptidases in the simulated digestion of milk proteins. Molecular Nutrition and Food Research, 59(5), 948–956. https://doi.org/10.1002/mnfr.201400856

10. Vieira, E.F., das Neves, J., Vitorino, R., Dias da Silva, D., Carmo, H., Ferreira, I.M. (2016). Impact of in vitro Gastrointestinal Digestion and Transepithelial Transport on Antioxidant and ACE-Inhibitory Activities of Brewer’s Spent Yeast Autolysate. Journal of Agricultural and Food Chemistry, 64(39), 7335–7341. https://doi.org/10.1021/acs.jafc.6b02719

11. Mora, L., Bolumar, T., Heres, A., Toldrá, F. (2017). Effect of cooking and simulated gastrointestinal digestion on the activity of generated bioactive peptides in aged beef meat. Food and Function, 8(12), 4347–4355. https://doi.org/10.1039/C7FO01148B

12. Aspri, M., Leni, G., Galaverna, G., Papademas, P. (2018). Bioactive properties of fermented donkey milk, before and after in vitro simulated gastrointestinal digestion. Food Chemistry, 268, 476–484. https://doi.org/10.1016/j.foodchem.2018.06.119

13. Chakrabarti, S., Guha, S., Majumder, K. (2018). Food-De‑ rived Bioactive Peptides in Human Health: Challenges and Op‑ portunities. Nutrients, 10(11), 1738. https://doi.org/10.3390/nu10111738

14. Lisitsyn, A.B., Chernukha, I.M., Fedulova, L.V., Kotenkova, E. A. Functional meat product and its manufacture method. Pa‑ thent RF, no. 2550649, 2015. (in Russian)

15. Kotenkova Е. А. (2015). Application of biotechnological and proteomic methods in the development of food products with hypolipidemic and vasoprotective effects. Author’s abstract of the dissertation for the scientific degree of Candidate of Technical Sciences. Moscow: VNIIMP. — 22 p. (in Russian)

16. Chernukha, I., Fedulova, L., Kotenkova, E., Akhremko, A. (2018). Hypolipidemic action of the meat product: in vivo study. Potravinarstvo Slovak Journal of Food Sciences, 12(1), 566–569. https://doi.org/10.5219/959

17. Chernukha, I.M., Kotenkova, E.A. (2018). Ifluence of functional food product on serum fatty acid composition in hyperlipidemic rats. Food systems, 1(4), 4–9. https://doi.org/10.21323/2618–9771–2018–1–4–4–9 (in Russian)

18. Kotenkova, E.A., Fedulova, L.V., Chernukha, I.M. (2017). The study of isolated from sus scrofa aorta tissue-specific substances with a molecular weight less than 30 kDa. Vsyo o myase, 2, 40–42. (in Russian)

19. Fedulova, L.V., Vasilevskaya, E.R., Kotenkova, E.A., Kalinova, E.A. (2018). Algorithm of in vitro assessment for products containing bioactive substances. Vsyo o myase, 6, 47–49. https://doi.org/10.21323/2071–2499–2018–6–47–49. (in Russian)

20. Vasilevskaya, E.R., Kotenkova, E.A., Lukoniva, E.A., Kalinova, E.A. (2017). Research methodology of Sus Scrofa tissue extracts protein-peptide components. Theory and practice of meat processing, 2(3), 79–85. https://doi.org/10.21323/2414–438X‑2017–2–3–79–85 (in Russian)

21. Li, L., Liu, Y., Zhou, G., Xu, X., Li, C. (2017). Proteome Profiles of Digested Products of Commercial Meat Sources. Frontiers in Nutrition, 4, 8. https://doi.org/10.3389/fnut.2017.00008

22. Wen, S., Zhou, G., Song, S., Xu, X., Voglmeir, J., Liu, L., Zhao, F., Li, M., Li, L., Yu, X., Bai, Y., Li, C. (2015). Discrimination of in vitro and in vivo digestion products of meat proteins from pork, beef, chicken, and fish. Proteomics, 15(21), 3688–3698. https://doi.org/10.1002/pmic.201500179.


Review

For citations:


Vasilevskaya E.R., Akhremko A.G., Polishchuk E.K., Fedulova L.V. Study of the functional product’s protein compounds digestion features. Theory and practice of meat processing. 2020;5(3):18-21. https://doi.org/10.21323/2414-438X-2020-5-3-18-21

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