IMPLEMENTATION OF IN VITRO ASSAY FOR EVALUATION OF BIOCORRECTIVE ACTION OF BIOACTIVE SUBSTANCES AN ANIMAL ORIGIN


https://doi.org/10.21323/2414-438X-2016-1-4-4-10

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Abstract

Slaughter products are good source of bioactive substances with different biological effects. Nowadays, approximately 220 functional peptides consisting of 2–10 amino acid residues have been identified. In this study, the biological activity of medium-molecular-weight substances (5–30 kDa, Mw) and low-molecular-weight substances (less than 5 kDa, Mw) extracted from aorta of Sus scrofa were investigated in vitro. Medium-molecular-weight and low-molecular-weight fractions possessed a tissue specific effect on organotypic tissue cultures of chicken embryos and laboratory rats. Biological activity depended on molecular weight. Medium-molecular-weight fractions possessed higher effect on aortas explants of chicken embryo: area index of cellular monolayer was 56.6±5.2 % (area index of the monolayer was 37.47±3.27 % for low-molecularweight fraction) in comparison with control explants. The low-molecular-weight fractions stimulated aortic tissues explants of aging rats — area index exceeded area index formedium-molecularweight fractions by more than 30%.


About the Authors

L. V. Fedulova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation

Fedulova Lilija  — candidate of technical sciences, head of Experimental clinic — laboratory of biologically active substances of an animal origin,

109316, Moscow, Talalikhina str., 26



E. V. Kashinova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation

Kashinova Elta  — research scientist of Experimental clinic  — laboratory of biologically active substances of an animal origin,

109316, Moscow, Talalikhina str., 26



Е. A. Kotenkova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation

Kotenkova Elena — candidate of technical sciences, research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin, 

109316, Moscow, Talalikhina str., 26



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Supplementary files

For citation: Fedulova L.V., Kashinova E.V., Kotenkova Е.A. IMPLEMENTATION OF IN VITRO ASSAY FOR EVALUATION OF BIOCORRECTIVE ACTION OF BIOACTIVE SUBSTANCES AN ANIMAL ORIGIN. Theory and practice of meat processing. 2016;1(4):4-10. https://doi.org/10.21323/2414-438X-2016-1-4-4-10

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