IMPLEMENTATION OF IN VITRO ASSAY FOR EVALUATION OF BIOCORRECTIVE ACTION OF BIOACTIVE SUBSTANCES AN ANIMAL ORIGIN
Abstract
Slaughter products are good source of bioactive substances with different biological effects. Nowadays, approximately 220 functional peptides consisting of 2–10 amino acid residues have been identified. In this study, the biological activity of medium-molecular-weight substances (5–30 kDa, Mw) and low-molecular-weight substances (less than 5 kDa, Mw) extracted from aorta of Sus scrofa were investigated in vitro. Medium-molecular-weight and low-molecular-weight fractions possessed a tissue specific effect on organotypic tissue cultures of chicken embryos and laboratory rats. Biological activity depended on molecular weight. Medium-molecular-weight fractions possessed higher effect on aortas explants of chicken embryo: area index of cellular monolayer was 56.6±5.2 % (area index of the monolayer was 37.47±3.27 % for low-molecularweight fraction) in comparison with control explants. The low-molecular-weight fractions stimulated aortic tissues explants of aging rats — area index exceeded area index formedium-molecularweight fractions by more than 30%.
About the Authors
L. V. Fedulova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation
Russian Federation
Fedulova Lilija — candidate of technical sciences, head of Experimental clinic — laboratory of biologically active substances of an animal origin,
109316, Moscow, Talalikhina str., 26
E. V. Kashinova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation
Russian Federation
Kashinova Elta — research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin,
109316, Moscow, Talalikhina str., 26
Е. A. Kotenkova
V.M. Gorbatov All-Russian Meat Research Institute
Russian Federation
Russian Federation
Kotenkova Elena — candidate of technical sciences, research scientist of Experimental clinic — laboratory of biologically active substances of an animal origin,
109316, Moscow, Talalikhina str., 26
References
1. Ahhmed A.M. A review of meat protein hydrolysates and hypertension/ A.M. Ahhmed, M. Muguruma // Meat science. — 2010. — № 86. — Р. 110–118.
2. Lafarga T. Bioactive peptides from meat muscle and byproducts: generation, functionality and application as functional ingredients / T. Lafarga, M. Hayes // Meat science. — 2014. — № 98. — Р. 227–239.
3. Mine Y. Nutraceutical Science and Technology (Book 4). Nutraceutical Proteins and Peptides in Health and Disease / Y. Mine, F. Shahidi // USA: Taylor & Francis Group, — 2006. — P. 688.
4. Bauchart, C. Small peptides (<5 kDa) found in ready–to–eat beef meat / C. Bauchart, D. Re´mond, C. Chambon, P. Patureau Mirand, I. Savary–Auzeloux, C. Reyne`s, M. Morzel // Meat science. — 2006. — № 74. — Р. 658–666.
5. Udenigwe C.C. Meat proteome as source of functional biopeptides / C.C. Udenigwe, A. Howard // Food Research International. — 2013. — № 54. — Р. 1021–1032.
6. Chernukha I.М. Effect of tissue specific biomolecules on endothelial dysfunction in atherosclerosis / I.М. Chernukha, L.V. Fedulova, Е.А. Kotenkova // Vse o myase. — 2016. — № 1. — P. 46–49.
7. Kotenkova Е.А. Use of the biotechnological and proteomic methods in development of food products with hypolipidemic and vasoprotective action: author’s abstract of the dissertation … candidate of technical sciences: 05.18.04, 05.18.07 / Kotenkova Е.А. [The venue of defense: The V.M. Gorbatov All-Russian Meat Research Institute]. — Moscow, 2015. — P. 27.
8. Nazarenko А.B. A method for obtaining a biologically active complex and biologically active protein-polypeptide complex / А.B. Nazarenko, М.А. Sokolov, I.N. Zhukova // RF Patent 2428196 of 10.09.2011
9. Khavinson V.Kh. Tissue specific action of peptides in the tissue culture of rats of different ages / V.Kh. Khavinson, V.V. Malinin, N.I. Chalisova, Е.I. Grigoriev// Uspekhi Gerontologii (Advances in Gerontology) — 2002. — Vol. 3. — № 9. — P. 248–252.
Review
For citation:
Fedulova L.V., Kashinova E.V., Kotenkova Е.A. IMPLEMENTATION OF IN VITRO ASSAY FOR EVALUATION OF BIOCORRECTIVE ACTION OF BIOACTIVE SUBSTANCES AN ANIMAL ORIGIN. Theory and practice of meat processing. 2016;1(4):4-10. https://doi.org/10.21323/2414-438X-2016-1-4-4-10
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