Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s
https://doi.org/10.21323/2414-438X-2022-7-2-125-130
Abstract
There is constant necessity of developing the accurate and fast methods for detection of foodborne pathogens. Microorganisms of Campylobacter genus are one of the main causes of foodborne diseases worldwide. Fast identification of Campylobacter at all stages of the food life cycle is an efficient strategy to control foodborne campylobacteriosis. This article the authors evaluated a commercial loop-mediated isothermal amplification (LAMP) system with bioluminescence, called as the 3M™ Molecular Detection Analysis (MDA), which was used to find Campylobacter in food products with the help of a certain standard method, which is referred to as the reference method. The results of this study showed that the commercial LAMP based method is as efficient as the reference method, and features high specificity and minimum determinability (sensitivity). The LAMP based method has been shown to be a fast and reliable method for detection of Campylobacter spp. scarce presence (10 CFU/25 g) in meat, meat products, as well as carcass swabs and production facilities’ environment. The LAMP analysis required about 24–27 hours to achieve a result. However the LAMP based method will facilitate the detection of Campylobacter, as it provides much easier and faster detection of Cam pylobacter spp., including Campylobacter jejuni/Campylobacter coli, than standard microbiological methods. The LAMP based method is an efficient tool to prevent the spreading of Campylobacter spp. contamination in food products.
Keywords
bacteria of the genus Campylobacter,
PCR,
loop isothermal amplification method (LAMP),
poultry and meat processing enterprises
Supporting agencies: This work was supported by a grant from the Ministry of Science and Higher Education of the Russian Federation for large scientific projects in priority areas of scientific and technological development (grant number 075–15–2020–775)
About the Authors
Yu. K. Yushina
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Yulia K. Yushina, Candidate of Technical Sciences, Docent, Deputy Head of Laboratory «Center for food and feed testing»
26, Talalikhina str., 109316, Moscow
E. V. Zajko
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Elena V. Zayko, Researcher, Department of Hygiene of Production and Microbiology
26, Talalikhina str., 109316, Moscow
M. A. Grudistova
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Maria A. Grudistova, Researcher, Department of Hygiene of Production and Microbiology
26, Talalikhina str., 109316, Moscow
M. D. Reshchikov
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Maksim D. Reshchikov, Senior Laboratory Assistant, Department of Hygiene of Production and Microbiology
26, Talalikhina str., 109316, Moscow
N. A. Nasyrov
V. M. Gorbatov Federal Research Center for Food Systems
Russian Federation
Russian Federation
Nazarbay A. Nasyrov, Researcher, Department of Hygiene of Production and Microbiology
26, Talalikhina str., 109316, Moscow
D. V. Nikitchenko
Peoples' Friendship University of Russia
Russian Federation
Russian Federation
Dmitriy V. Nikitchenko, Doctor of Biological Sciences, Docent, Professor, Department of Veterinary Medicine
6, Miklukho-Maklaya str., 117198, Moscow
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Review
For citations:
Yushina Yu.K., Zajko E.V., Grudistova M.A., Reshchikov M.D., Nasyrov N.A., Nikitchenko D.V. Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s. Theory and practice of meat processing. 2022;7(2):125-130. https://doi.org/10.21323/2414-438X-2022-7-2-125-130
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