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Assessment of genotoxic and mutagenic effects of food products by bioassay methods

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The current state of studies on application and safety of food additives in various technologies for food production is examined. Considerable attention should be given to studies dedicated to analysis of food safety criteria due to a possibility of appearance of adverse consequences for human health and the trend towards increasing life quality. Special emphasis is placed on such parameters as genotoxicity and mutagenicity. It is shown that the most rapid and convenient tool for complex monitoring of product toxicity can be the bioassay procedure. Based on the review of the literature on bioassays for edible meat and meat products, canned foods, carbonated soft drinks, beer, milk and milk containing products as well as seasonings, the authors show that above mentioned products had the cyto- and genotoxic potential when tested on animal and human cell cultures, microorganisms and plants. With that, it was found that a list of relevant publications is quite small despite a significant growth in scientific research dedicated to food toxicity assessment using bioassays. A review on the conducted research on assessment of genotoxic and mutagenic effects of foods by bioassay methods will make it possible to extend the understanding of the processes and mechanisms of this toxicity and form more rational concept of consumption.

About the Author

M. V. Zaytseva
Russian Research Institute of Canning Technology Vidnoe
Russian Federation

Mariya V. Zaytseva —  junior researcher

Shkolnaia Str. 78, 142703, Vidnoe, Moscow region

Tel.: +7–996–964–58–29


1. Kuznetsova, O.A., Dydykin, A.S., Aslanova, M.A. (2018). High priority scientific research in the field of population nutrition. Meat Indusrty, 7, 8–12. (In Russian)

2. Nepriyatel, A.A (2020). The effect of the technology of maral material preservation on its biochemical and biological properties. Bulletin of KSAU, 8(161), 130–134.–4036–2020–8–130–134 (In Russian)

3. Demeyer, D., Mertens, B., De Smet, S., Ulens, M. (2016). Mechanisms linking colorectal cancer to the consumption of (processed) red meat: A review. Critical Reviews in Food Science and Nutrition,56(16), 2747–2766.

4. Chiang, V. S. C., Quek, S. Y. (2017). The relationship of red meat with cancer: Effects of thermal processing and related physiological mechanisms. Critical Reviews in Food Science and Nutrition,57(6), 1153–1173.

5. Marques, G. S., da Silva, S. I. O., e Sousa, J. M. C., Ferreira, P. M. P., Peron, A. P., Peron, A. P. (2015). Cytotoxic and genotoxic potential of liquid synthetic food flavorings evaluated alone and in combination. Food Science and Technology, 35(1), 183–188.–457X.6596

6. Gomes, K. M. S., de Oliveira, M. V. G. A., Carvalho, F. R. S., Menezes, C. C., Peron, A. P. (2013). Citotoxicity of food dyes sunset yellow (E-110), bordeaux red (E-123), and tatrazine yellow (E-102) on allium cepa L. root meristematic cells. [Citotoxicidade dos corantes alimentares amarelo crepúsculo (E-110), vermelho bordeaux (E-123) e amarelo tartrazina (E-102) em células meristemáticas de raízes de Allium cepa L] Food Science and Technology,33(1), 218–223.–20612013005000012

7. Prokhorenko S. Yu.,, Zhukova, A. Yu. (2018). Food colorant’’s review for meat products. Health or harm? Vsyo o myase, 3, 40–45.–2499–2018–3–40–45. (In Russian)

8. de Oliveira, M. V. A., Alves, D. D. L., Lima, L. H. G. M., de Castro, J. M., e Sousa, T. M. C., Peron, A. P. (2013). Cytotoxicity of erythrosine (E-127), brilliant blue (E-133) and red 40 (E-129) food dyes in a plant test system. [Citotoxicidade dos corantes alimentares erythrosine (E-127), azul brilhante (E-133) e red 40 (E-129) em sistemateste vegetal] Acta Scientiarum — Biological Sciences, 35(4), 557–562.

9. Aissa, A. F., Bianchi, M. L. P., Ribeiro, J. C., Hernandes, L. C., de Faria, A. F., Mercadante, A. Z. et al. (2012). Comparative study of β-carotene and microencapsulated β-carotene: Evaluation of their genotoxic and antigenotoxic effects. Food and Chemical Toxicology, 50(5), 1418–1424.

10. Wang, Y., Yuan, L., Yao, C., Ding, L., Li, C., Fang, J. et al. (2014). A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives. Nanoscale,6(24), 15333–15342.

11. Hudson, A., Lopez, E., Almalki, A. J., Roe, A. L., Calderón, A. I. (2018). A review of the toxicity of compounds found in herbal dietary supplements. Planta Medica, 84(9–10), 613–626.–3786

12. Abakumova, O. Yu., Kalinina, A.G., Samojlik, L.V., Kondrashev, G.I. (2015). The use of cultivated human cells for testing cytotoxicity alcohol drinks in the experiments in vitro. European Science, 9(10), 8–13. (In Russian)

13. da Silva, E. L., Sales, I. M. S., dos Santos, F. K. S., Peron, A. P. (2017). Processed fruit juice ready to drink: Screening acute toxicity at the cellular level. [Sucos de frutas industrializados prontos para consumo: Screening da toxicidade aguda em nível celular] Acta Scientiarum — Biological Sciences, 39(2), 195–200.

14. Atri, R., Singh, A., Mathur, N., Verma, A., Sharma, S. (2014). Analysis of genotoxic and cytotoxic potential of canned food products using microbial bioassay. International Journal of Advanced Biotechnology and Research,15(2), 141–150.

15. Khotimchenko, S. A., Gmoshinski, I.V., Bagryantseva, O.V., Shatrov, G. N. (2020). Chemical food safety: development of methodological and regulatory base. Problems of Nutrition, 89(4), 110124.–8833–2020–10047(In Russian)

16. EFSA panel of food additives and nutrient sources added to food (ANS) (2012). Guidance for submission for food additive evaluations. ESFA Journal, 10(7), 2760.

17. Avior, Y., Eggan, K., Benvenisty, N. (2019). Cancer-related mutations identified in primed and naive human pluripotent stem cells. Cell Stem Cell, 25(4), 456–461.

18. Alov, I.A. (1972). Cytophysiology and pathology of mitosis. Moscow: Medicine, 1972 (In Russian)

19. Augustsson, K., Lindblad, J., Overvik, E., Steineck, G. (1999). A population-based dietary inventory of cooked meat and assessment of the daily intake of food mutagens. Food Additives and Contaminants, 16(5), 215–225.

20. Sflomos, C., Papadopoulou, R., Athanasiou, K. (1989). Temperature and time effects on mutagen production in cooked lamb meat. Mutagenesis,4(3), 228–229.

21. Gocke, E., Eckhardt, K., King, M. -T., Wild, D. (1982). Mutagenicity study of fried sausages in salmonella, drosophila and mammalian cells in vitro and in vivo. Mutation Research/Genetic Toxicology, 101(4), 293–304.–1218(82)90122–7

22. Vikse, R., Joner, R. E. (1993). Mutagenicity, creatine and nutrient contents of pan fried meat from various animal species. Acta Veterinaria Scandinavica, 34(4), 363–370.

23. Fiskesjö, G. (1985). The allium test as a standard in environmental monitoring. Hereditas,102(1), 99–112.–5223.1985.tb00471.x

24. Bezerra, M. S., Malaquias, G. S., Castrod E Sodusa, J. M., Perodn, A. P. (2016). Cytotoxic and genotoxic potential of powdered juices. Food Science and Technology,36(1), 49–55.–457X.0006

25. Adams, F. M., Njoku, K., Famotemi, A. C., Idowu, D., Tijani, A. A. (2017). Genotoxic studies of cooked and uncooked processed spices using Allium cepa test. International Journal of Advanced Research in Biological Sciences,4(9), 21–39.

26. Carvalho, B. L., Sales, I. M. S., Peron, A. P. (2017). Cytotoxic, genotoxic and mutagenic potential of UHT whole milk. Food Science and Technology, 37(2), 275–279.–457X.21916

27. Moura, R. A. M., Marques, M. M. M., Pereira, H. M., Sales, I. M. S., Santos, F. K. S., Guedes, M. I. F. et al. (2017). Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity. [Leites industrializados, tipo em pó, frente a bioensaios de avaliação de citotoxicidade e genotoxicidade] Bioscience Journal, 33(6), 1622–1631.–37266

28. Farhan, E.M., Tawfiq, R.F. (2016). Detection of cytotoxicity and carcinogenicity of soft drink “Cola” on Allium Cepa root cells. International Journal of Advances in Agricultural and Environmental Engineerin,3(1), 92–95.

29. Chandraker, S.K., Singh, P., Pandey, B. (2014). Clastogenic effect of soft drink on root tip of Allium cepa. International Journal of Current Microbiology and Applied Sciences, 3(5), 200–206.

30. Hannah, C., Priya, E. J. S., Mammen, A. (2010). Duration dependent mutagenic study of cola drinks on allium cepa L. Biosciences Biotechnology Research Asia, 7(2), 807–812.

31. Samoylov, A.V., Suraeva, N.M., Zaytseva, M.V., Kurbanova, M.N., Belozerov, G.A. (2020). Toxicity of apple juice and its components in the model plant system. Foods and Raw materials, 8(2), 321328.–4057–2020–2–321–328

32. de Queiroz, F.M., de Oliveira Matias, K.W., Cunha, M.M., Schwarz, A. (2015). Evaluation of (anti) genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test. Brazilian Journal Pharmacological Science,49(1), 135–148.–82502013000100015

33. Rank, J. (2003). The method of Allium anaphase-telophase chromosome aberration assay. Ekologija,418, 38–42.

34. Jaruratanasirikul, S., Kleepkaew, A. (1997). Influence of an acidic beverage (coca-cola) on the absorption of itraconazole. European Journal of Clinical Pharmacology, 52(3), 235–237.

For citation:

Zaytseva M.V. Assessment of genotoxic and mutagenic effects of food products by bioassay methods. Theory and practice of meat processing. 2021;6(2):191-195.

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