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Techno-functional, textural and sensorial properties of frankfurters as affected by the addition of bee pollen powder

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The objective of this study was to determine whether the addition of different pollen powder concentrations (0.5; 1.0 and 1.5 g/100 g) had an influence on techno-functional, textural and sensorial traits of frankfurters. Examining the techno-functional characteristics of pollen, a conclusion was reached that the higher the concentration, the higher the emulsification and better techno-functional properties. Also, FTIR-ATR analysis has shown that specific pollen molecules provided good emulsifying properties of sausages. On the other hand, sensory analysis showed that sausages with the addition of 1.0% and 1.5% of pollen powder have a more pronounced floral odor. Warner-Bratzler shear force test has shown that the incorporation of pollen caused a more stable product throughout sixty days of storage than the control sample. It could be explained by the formation of more protein-protein interactions due to the addition of non-meat proteins in the formulation of frankfurters and obtaining a more stable product than the control one. All things considered, it can be concluded that pollen exhibits good techno-functional properties and could be utilized in the formulation of frankfurters with improved and steady techno-functional properties during two months of refrigerated storage.

About the Authors

S. M. Novakovic
University of Belgrade, Faculty of Agriculture

Sasa M. Novakovic —   PhD candidate, MSc, teaching assistant, Animal Source Food Technology Department

 6 Nemanjina, Zemun, 11080, Belgrade 

I. V. Djekic
University of Belgrade, Faculty of Agriculture

Ilija V. Djekic —  PhD, full professor, full professor, Department for Food Safety and Quality Management

6 Nemanjina, Zemun, 11080, Belgrade

M. B. Pesic
University of Belgrade, Faculty of Agriculture

Mirjana B. Pesic —   PhD, associate professor, associate professor, Department for Chemistry and Biochemistry

6 Nemanjina, Zemun, 11080, Belgrade

A. Z. Kostic
University of Belgrade, Faculty of Agriculture

Aleksandar Z. Kostic —  PhD, assistant professor, Department for Chemistry and Biochemistry

6 Nemanjina, Zemun, 11080, Belgrade

D. D. Milincic
University of Belgrade, Faculty of Agriculture

Danijel D. Milincic —  PhD candidate, MSc, teaching assistant, Department for Chemistry and Biochemistry

6 Nemanjina, Zemun, 11080, Belgrade

I. В. Tomasevic
University of Belgrade, Faculty of Agriculture

Igor B. Tomasevic —  PhD, associate professor, associate professor, Animal Source Food Technology Department

6 Nemanjina, Zemun, 11080, Belgrade


1. Fernández-López, J., Lucas-González, R., Viuda-Martos, M., Sayas-Barberá, E., Navarro, C., Haros, C. M., Pérez-Álvarez, J. A. (2019). Chia (Salvia hispanica L.) products as ingredients for reformulating frankfurters: Effects on quality properties and shelflife. Meat Science, 156, 139–145.

2. Feiner, G. (2006). Meat products handbook: Practical science and technology. Elsevier.3. Novakovic, S., Djekic, I., Klaus, A., Vunduk, J., Djordjevic, V., Tomović, V. et. al. (2019). The Effect of Cantharellus Cibarius Addition on Quality Characteristics of Frankfurter during Refrigerated Storage. Foods, 8(12), Article 635.

3. Novakovic, S., Djekic, I., Klaus, A., Vunduk, J., Đorđević, V., Tomovic, V. et. al. (2020). Application of porcini mushroom (Boletus edulis) to improve the quality of frankfurters.Journal of Food Processing and Preservation, 44(8), Article e14556.

4. Alirezalu, K., Hesari, J., Yaghoubi, M., Khaneghah, A. M., Alirezalu, A., Pateiro, M., Lorenzo, J. M. (2021). Combined effects of ε-polylysine and ε-polylysine nanoparticles with plant extracts on the shelf life and quality characteristics of nitrite-free frankfurtertype sausages. Meat Science, 172, Article 108318.

5. Horita, C. N., Farías-Campomanes, A. M., Barbosa, T. S., Esmerino, E. A., da Cruz, A. G., Bolini, H. M. A. et. al. (2016). The antimicrobial, antioxidant and sensory properties of garlic and its derivatives in Brazilian low-sodium frankfurters along shelf-life. Food Research International, 84, 1–8.

6. Kostić, A., Milinčić, D., Barać, M., Ali Shariati, M., Tešić, Ž., Pešić, M. (2020). The Application of Pollen as a Functional Food and Feed Ingredient — The Present and Perspectives. Biomolecules, 10(1), Article 84.

7. Thakur, M., Nanda, V. (2020). Exploring the physical, functional, thermal, and textural properties of bee pollen from different botanical origins of India. Journal of Food Process Engineering, 43(1), Article e12935.

8. Karabagias, I. K., Karabagias, V. K., Gatzias, I., Riganakos, K. A. (2018). Bio-Functional Properties of Bee Pollen: The Case of “Bee Pollen Yoghurt”. Coatings, 8(12), Article 423.

9. Abd Elhamid, A. M., Elbayoumi, M. M. (2017). Influence of bee pollen on the bioactive behavior, sensory and physicochemical properties of white cheese made from camel and cow milk mixture. Journal of Food Dairy Sciences, 8(11), 419–424.

10. Di Cagno, R., Filannino, P., Cantatore, V., Gobbetti, M. (2019). Novel solid-state fermentation of bee-collected pollen emulating the natural fermentation process of bee bread. Food Microbiology, 82, 218–230.

11. de Florio Almeida, J., dos Reis, A. S., Heldt, L. F. S., Pereira, D., Bianchin, M., de Moura, C. et. al. (2017). Lyophilized bee pollen extract: A natural antioxidant source to prevent lipid oxidation in refrigerated sausages. LWT-Food Science Technology, 76, 299–305.

12. Kostić, A., Barać, M., Stanojević, S., Milojković-Opsenica, D., Tešić, Ž., Šikoparija, B. et. al. Physicochemical composition and techno-functional properties of bee pollen collected in Serbia. LWT-Food Science Technology, 62(1), 301–309.

13. Costa-Lima, B. R. C., Canto, A. C. V. C. S., Suman, S. P., Conte-Junior, C. A., Silveira, E. T. F., Silva, T. J. P. (2014). Sex-specific effect of ractopamine on quality attributes of pork frankfurters. Meat Science, 96(2, Part A), 799–805.

14. De Huidobro, F. R., Miguel, E., Blázquez, B., Onega, E. (2005). A comparison between two methods (Warner–Bratzler and texture profile analysis) for testing either raw meat or cooked meat. Meat Science, 69(3), 527–536.

15. Candoğan, K., Altuntas, E. G., İğci, N. (2021). Authentication and Quality Assessment of Meat Products by Fourier-Transform Infrared (FTIR) Spectroscopy. Food Engineering Reviews, 13(1), 66–91.–020–09251-y

16. Deniz, E., Güneş Altuntaş, E., Ayhan, B., İğci, N., Özel Demiralp, D., Candoğan, K. (2018). Differentiation of beef mixtures adulterated with chicken or turkey meat using FTIR spectroscopy. Journal of Food Processing Preservation, 42(10), Article e13767.

17. Andrade, J., Pereira, C. G., de Almeida Junior, J. C., Viana, C. C. R., de Oliveira Neves, L. N., da Silva, P. H. F. et. al. (2019). FTIR-ATR determination of protein content to evaluate whey protein concentrate adulteration. LWT, 99, 166–172.

18. Kasprzyk, I., Depciuch, J., Grabek-Lejko, D., Parlinska-Wojtan, M. (2018). FTIR-ATR spectroscopy of pollen and honey as a tool for unifloral honey authentication. The case study of rape honey. Food Control, 84, 33–40.

19. Kostić, A. Ž., Milinčić, D. D., Gašić, U. M., Nedić, N., Stanojević, S. P., Tešić, Ž. L., Pešić, M. B. (2019). Polyphenolic profile and antioxidant properties of bee-collected pollen from sunflower (Helianthus annuus L.) plant. LWT, 112, Article 108244.

20. Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U. M., Lević, S., Kojić, M. O. at al. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. Food Chemistry, 351, Article 129310.

21. Jin, S.-K., Choi, J. S., Yang, H.-S., Park, T.-S., Yim, D.-G. (2018).Natural curing agents as nitrite alternatives and their effects on the physicochemical, microbiological properties and sensory evaluation of sausages during storage. Meat Science, 146, 34–40.

22. Silva, F. A. P., Amaral, D. S., Guerra, I. C. D., Arcanjo, N. M. O., Bezerra, T. K. A., Ferreira, V. C. S. et. al. (2014). Shelf life of cooked goat blood sausage prepared with the addition of heart and kidney. Meat Science, 97(4), 529–533.

23. Herrera, E. A. C. (2006). Contributions to the characterization of the black pudding of León and evolution of certain physical, chemical and microbiological parameters during its conservation under refrigeration.(theses of the dissertation) (In Spanish)

24. Miller, R. K. (2012). Sensory Evaluation of Beef Flavor. Chapter in a book: Handbook of Meat, Poultry and Seafood Quality (pp. 173–191). Blackwell Publishing Ltd.

25. Galán-Soldevilla, H., Ruiz-Pérez-Cacho, M. P., Serrano Jiménez, S., Jodral Villarejo, M., Manzanares, A. B. (2005). Development of a preliminary sensory lexicon for floral honey. Food Quality and Preference, 16(1), 71–77.

For citation:

Novakovic S.M., Djekic I.V., Pesic M.B., Kostic A.Z., Milincic D.D., Tomasevic I.В. Techno-functional, textural and sensorial properties of frankfurters as affected by the addition of bee pollen powder. Theory and practice of meat processing. 2021;6(2):135-140.

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