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Polymorphism of the gene GDF9 in sheep of Prikatun type of Altai Mountains breedand its correlation with indices of meat rate productivity

https://doi.org/10.21323/2414-438X-2021-6-1-4-9

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Abstract

In recent decades in many countries of the world the development of sheep farming is determined by the efficiency of mutton production. One of the promising areas of selection improvement of sheep breeds of meat and meat & wool productivity is an application of molecular-genetic methods. To obtain high-quality mutton it is advisable to use the Altai Mountains sheep breed, which features by high energy of growth of young animals and their adaptability to all-year-round pasture management. One of the candidate genes responsible for sheep meat productivity is the gene of growth differentiation factor -GDF9 (growth differentia­tion factor 9). The article presents the results of research of polymorphism of the gene GDF9 and its relation with the parameters of meat productivity in young sheep of Altai Mountains breed. It was found that the rams-carriers of AA genotype outperformed their peers of AG and GG genotypes in terms of pre-slaughter weight, carcass weight, meat yield and meat content ratio by 1.62 and 7.01 kg, 1.34 and 3.98 kg (P<0.05), 1.21 and 1.86 abs. percent, 0.16 and 0.39 units (P<0.05) accordingly. The muscle tissue of rams of the desired genotype featured the highest content of protein, fat and, accordingly, energy value in comparison with AG and GG genotypes. The animals of AA genotype had the largest area of a rib eye and diameter of muscle fibers. The superiority over other genotypes achieved 1.55 cm2 and 1.98 microns in average. In addition the higher number of inter-fiber and inter-bundle inclusions of fat and lower content of connective tissue stipulated higher marbling score (MB)-higher by 3.32 points. The obtained data in­dicate that it is promising methods to increase the number of homozygotic animals in term of gene GDF9 by intended selection of parent animals whose genotype contains a desirable element for further improvement of quantitative and qualitative parameters of meat rate productivity in sheep of Altai Mountains breed.

About the Authors

M. I. Selionova
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy
Russian Federation

Селионова Марина Ивановна - доктор биологических наук, профессор, заведующая кафедрой селекции, генетики и биотехнологии животных.

127550, г. Москва, ул. Тимирязевская, 49. Тел .: + 7–968–266–33–03.



N. A. Podkorytov
Altai Mountains Research Institute of Agriculture — Branch of the Federal Altai Scientific Center of Agrobiotechnology
Russian Federation

Николай А. Подкорытов - кандидат сельскохозяйственных наук, заведующий кафедрой овцеводства.

649100, Республика Алтай, р. Майма, ул. Катунская, 2,
тел .: + 7–913–237–16–76



References

1. Zinovieva, N. A., Kostyunina, O. V., Gladyr, E. A., Bannikova, A.D., Kharzinova, V.R., Larionova, P.V. at al. (2010). The role of DNA markers of indices of productivity of agricultural animals. Zootechniya, 1, 9–10. (In Russian)

2. Ibtisham, F., Zhang, L., Xiao, M., An, L., Ramzan, M. B., Naw ab, A. at.al. (2017). Genomic selection and its application in ani mal breeding. Thai Journal of Veterinary Medicine, 47(3), 301–310

3. Georges, M., Charlier, C., Hayes, B. (2019). Harnessing genom ic information for livestock improvement. Nature Reviews genetics, 20(3), 135–156. https://doi.org/10.1038/s41576–018–0082–2

4. Meuwissen, T., Hayes, B., Goddard, M. (2016). Genomic se lection: A paradigm shift in animal breeding. Animal Frontiers, 6(1), 6–14. https://doi.org/ 10.2527/af.2016–0002

5. Kholmanov, A.M., Dankvert, S.A., Osadchaya, O. Yu. (2015). The number of sheep and the production of mutton in the world. Sheep, goats, wool business, 4, 15–20. (In Russian)

6. Wang, J., Zhou, H., Hu, J., Li, S., Luo, Y., Hickford, J. G. H. (2016). Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep. Journal of Animal Breeding and genetics, 133(3), 219–226. https://doi. org/10.1111/jbg.12171

7. Arora, R., Yadav, H., Yadav, D. (2014). Identification of novel single nucleotide polymorphisms in candidate genes for mutton quality in Indian sheep. Animal Molecular Breeding, 12(5), 45– 51. https://doi.org/10.5376/amb.2014.04.0001

8. Fang, Q., Forrest, R. H., Zhou, H., Frampton, C. M., Hick ford, J. G. H. (2013). Variation in exon 10 of the ovine calpain 3 gene (CAPN3) and its association with meat yield in New Zea land Romney sheep. Meat Science, 94(3), 388–390. https://doi. org/10.1016/j.meatsci.2013.03.015

9. Hajihosseinlo, A., Jafari, S., Ajdary, M. (2015). The relation of GH and LEP gene polymorphisms with fat-tail measurements (fat-tail dimensions) in fat-tailed Makooei breed of Iranian sheep. Advanced Biomedical Research, 4(1), 172181. https://doi. org/10.4103/2277–9175.163995

10. Saleem, A. H., Javed, K., Babar, M. E., Hussain, T., Ali, A., Ali, A. at al. (2018). Association of leptin gene polymorphism with growth rate in lohi sheep. Pakistan Journal of Zoology, 50(3), 1029–1033. https://doi.org/10.17582/journal.pjz/2018.50.3.1029.1033

11. Miar, Y., Salehi, A., Kolbehdari, D., Aleyasin, S.A. (2014). Ap plication of myostatin in sheep breeding programs: A review. Mo lecular Cell Biology Research Communications, 3(1), 3343.

12. Gorlov, I., Kolosov, Y., Shirokova, N., Getmantseva, L., Slozhenkina, M., Mosolova, N. at al. (2017). Association of the growth hormone gene polymorphism with growth traits in Salsk sheep breed. Small Ruminant Research, 150, 11–14. https://doi. org/10.1016/j.smallrumres.2017.02.019

13. Gorlov, I. F., Shirokova, N. V., Natyrov, A. K., Kolosov, Y. A., Slozhenkina, M. I., Kolosov, A. Y. at al (2021). Growth hormone (GH) gene polymorphism and its association with meat produc tivity in two rough wool sheep breeds grown in Russia’s dry zone. International Journal of Agriculture and Biology, 25(1), 255–259. https://doi.org/10.17957/IJAB/15.1664

14. Armstrong, E., Ciappesoni, G., Iriarte, W., Da Silva, C., Mace do, F., Navajas, E. A. at al. (2018). Novel genetic polymorphisms as sociated with carcass traits in grazing texel sheep. Meat Science, 145, 202–208. https://doi.org/10.1016/j.meatsci.2018.06.014

15. Akhatayeva, Z., Li, H., Mao, C., Cheng, H., Zhang, G., Jiang, F. at al. (2020). Detecting novel indel variants within the GHR gene and their associations with growth traits in luxi blackhead sheep. Animal Biotechnology, https://doi.org/10.1080/10495398.202 0.1784184

16. Jia, J. L., Zhang, L. P., Wu, J. P., Ha, Z. J., Li, W. W. (2014). Study of the correlation between GH gene polymorphism and growth traits in sheep. genetics and Molecular Research, 13(3), 7190–7200. https://doi.org/10.4238/2014.September.5.5

17. Valeh, M.V., Tahmoorespour, M., Ansari, M., Nassiry, M.R., Karimi, D., Taheri, A. (2012). Association of growth traits with SSCP polymorphisms at the growth hormone receptor (GHR) and growth hormone releasing hormone receptor (GHRHR) genes in the Baluchi sheep. Journal of Animal and Veterinary Advances, 8(6), 1063–1069.

18. Meira, A. N., Montenegro, H., Coutinho, L. L., Mourão, G. B., Azevedo, H. C., Muniz, E. N. at al. (2019). Single nucleotide poly morphisms in the growth hormone and IGF type 1 (IGF1) genes associated with carcass traits in Santa Ines sheep. Animal, 13(3), 460–468. https://doi.org/10.1017/S1751731118001362

19. Wu, M., Zhao, H., Tang, X., Li, Q., Yi, X., Liu, S., Sun, X. (2020). Novel indels of GHR, GHRH, GHRHR and their association with growth traits in seven Chinese sheep breeds. Animals, 10(10), 1–13. https://doi.org/ 10.3390/ani10101883

20. Wang, F., Chu, M., Pan, L., Wang, X., He, X., Zhang, R. at al. (2021). Polymorphism detection of gene GDF9 and its associa tion with litter size in Luzhong mutton sheep (ovis aries). Animals, 11(2), 1–11. https://doi.org/10.3390/ani11020571

21. Li, Y., Jin, W., Wang, Y., Zhang, J., Meng, C., Wang, H. at al. (2020). Three complete linkage SNPs of gene GDF9 affect the lit ter size probably mediated by OCT1 in hu sheep. DNA and Cell Bi ology, 39(4), 563–571. https://doi.org/10.1089/dna.2019.4984

22. Chu, M. X., Yang, J., Feng, T., Cao, G. L., Fang, L., Di, R. at al. (2011). GDF9 as a candidate gene for prolificacy of small tail Han sheep. Molecular Biology Reports, 38(8), 5199–5204. https://doi.org/10.1007/s11033–010–0670–5

23. Pokharel, K., Peippo, J., Honkatukia, M., Seppälä, A., Rauti ainen, J., Ghanem, N. at al. (2018). Integrated ovarian mRNA and miRNA transcriptome profiling characterizes the genetic basis of prolificacy traits in sheep (ovis aries). BMC Genomics, 19(1). https://doi.org/10.1186/s12864–017–4400–4

24. Pan, Z., Wang, X., Di, R., Liu, Q., Hu, W., Cao, X. at al. (2018). A 5-methylcytosine site of growth differentiation factor 9 (Gdf9) gene affects its tissue-specific expression in sheep. Animals, 8(11). https://doi.org/10.3390/ani8110200

25. Getmantseva, L., Bakoev, N., Shirokova, N., Kolosova, M., Ba koev, S., Kolosov, A. at al. (2019). Effect of the gene GDF9 on the weight of lambs at birth. Bulgarian Journal of Agricultural Sci ence, 25(1), 153–157.

26. Al-Khuzai, F. L. J., Ahmed, J. R. (2019). Polymorphism of GDF9 (exon 1) gene and its association with milk production and prolifi cacy of awassi sheep. Plant Archives, 19(2), 4037–4040.

27. Podkorytov, A. T., Selionova, M. I., Podkorytov, N. A, Podkory tov, A. A. (2018). Sheep and goat breeding in the Altai Republic: status, problems, solution. Zootechniya, 10, 8–11. (In Russian)

28. Khvylya, S.I., Pchelkina, V.A., Burlakova, S.S. (2011). Stan dardized histological methods for assessing the quality of meat and meat products. Vsyo o myase, 6, 32–35. (In Russian)

29. Selionova, M. I. Chizhova, L. N., Mikhailenko, A. K., Kvitko, Yu. D., et al. (2015). System of comprehensive assessment of the genetic potential of breeding animals, Stavropol, VNIIОK. — 50 p. (In Russian)

30. Selionova, M.I., Chizhova, L.N., Surzhikova, E.S., Podkorytov, N.A., Podkorytov, A.T., Voblikova, T.V. (2020). Meat productivity of sheep of Altai Mountains breed of different genotypes accord ing to the CAST and gene GDF9s. IOP Conference Series: Earth and Environmental Science, 613(1), Article 012130. https://doi. org/10.1088/1755–1315/613/1/012130

31. Kolosov, Y.А., Getmantseva, L.V., Shirockova, N.V., Klimenko, A., Bakoev, S. Y., Usatov, A.V. at al. (2015). Polymorphism of the gene GDF9 in Russian sheep breeds. Journal of Cytology & Histol ogy, 6(1). https://doi.org/10.4172/2157–7099.1000305

32. Bahrami, Y., Bahrami, S., Mohammadi, H. R., Chekani-Azar, V., Mousavizadeh, S.A. (2014). The polymorphism of GDF 9 gene in Hisari sheep. Biological Forum — An International Journal, 6(2), 46–52.


For citation:


Selionova M.I., Podkorytov N.A. Polymorphism of the gene GDF9 in sheep of Prikatun type of Altai Mountains breedand its correlation with indices of meat rate productivity. Theory and practice of meat processing. 2021;6(1):4-9. https://doi.org/10.21323/2414-438X-2021-6-1-4-9

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