Research of the physical and chemical properties and methods of Red rice (food colour) determination in sausage products
https://doi.org/10.21323/2414-438X-2020-5-4-23-28
Abstract
In Russia, in the production of meat and sausage products, the food colour, named as Red rice, is use. Red rice is obtain by the cultivation the strains of the Monascus fungus on various carbohydrate substrates, for example rice. That Red rice may contain the mycotoxin citrinin, but neither the purity of the food colour nor the safety profile are regulated. The aim of this work was to study the physical and chemical properties of Red rice and to develop method for its determination in sausage products. The experiments were carried out on model and commercial samples of sausages. The samples were analyzed using spectrophotometry and highefficiency planar and liquid chromatography. Spectrophotometric analysis revealed differences in solubility, coloring power, spectral characteristics, composition and ratio of pigments in commercial samples of Red rice. The extraction parameters of colouring substances from sausages model samples were determined. It has been established that Red rice is extracted with chloroform, acetone, ethanol and its aqueous solutions. Petroleum ether was proposed for degreasing sausages. Red rice from sausages was extracted with acetone because it did not extract synthetic colours and carminic acid. It was found, that 90% of Red rice is extracted from samples of sausages by double extraction with ultrasonic treatment with a power of 128 W. High performance thin layer chromatography method and high performance liquid chromatography method for the Red rice identification was proposed. The content of Red rice in the extracts was determined by spectrophotometric method. The developed method for the determination of Red rice was tested on commercial samples of sausage products.
About the Authors
N. V. Rudometova
All-Russia Research Institute for Food Additives — Branch of V. M. Gorbato Federal Research Center for Food Systems of RAS
Russian Federation
Natalia V. Rudometova — candidate of chemical sciences, leading research scientist, laboratory of physical and chemical research methods, 191014, Sankt-Petersburg, Liteyniy Prospect, 55. Tel. +7–812–272–75–78
Russian Federation
Natalia V. Rudometova — candidate of chemical sciences, leading research scientist, laboratory of physical and chemical research methods, 191014, Sankt-Petersburg, Liteyniy Prospect, 55. Tel. +7–812–272–75–78
I. S. Kim
All-Russia Research Institute for Food Additives — Branch of V. M. Gorbato Federal Research Center for Food Systems of RAS
Russian Federation
Irina S. Kim — junior research scientist, laboratory of physical and chemical research methods, 191014, Sankt-Petersburg, Liteyniy Prospect, 55. Tel. +7–812–272–56–26
Russian Federation
Irina S. Kim — junior research scientist, laboratory of physical and chemical research methods, 191014, Sankt-Petersburg, Liteyniy Prospect, 55. Tel. +7–812–272–56–26
References
1. Rudometova, N.V. (2014). Food additives and colorants: methodological support of product safety. Production quality control, 8, 13–19.
2. ТR ТS029/2012 Customs Union Technical Regulations “Safety requirements of food additives, flavorings and processing aids” [Electronic resource: http://www.eurasiancommission.org/ru/act/texnreg/deptexreg/tr/Pages/bezopPischDobavok.aspx / Access date 09.09.2020]
3. Agboyibor, C., Kong, W.-b., Chen, D., Zhang, A.-m., Niu, S-q. (2018). Monascus pigments production, composition, bioactivity and its application: A review. Biocatalysis and Agricultural Biotechnology, 16, 433–447. https://doi.org/10.1016/j.bcab.2018.09.012
4. Mhalaskar, S.R., Thorat, S.S., Deshmukh, Y.R. (2017). Standardization of fermentation parameters for the production of food bio-colours through submerged bio-reactor fermentation. Ecology, Environment and Conservation, 23(1), 211–216.
5. Srianta, I., Ristiarini, S., Nugerahani, I., Sen, S. K., Zhang, B. B., Xu, G. R., Blanc, P. J. (2014). Recent research and development of monascus fermentation products. International food research journal, 21(1), 1–12.
6. Hendry, G.A.F., Houghton, J.D. (1996). Natural food colorants. Blackie Academic & Professional. — 350 p. ISBN978–0–7514–0231–5, https://doi.org/10.1007/978–1–4615–2155–6
7. Xu, B.-j., Jia, X.-j., Gu, L.-j., Sung, C.-k. (2006). Review on the qualitative and quantitative analysisof the mycotoxin citrinin. Food Control, 17(4), 271–285. https://doi.org/10.1016/j.foodcont.2004.10.012
8. Commission Regulation (EU) № 212/2014 of 6 March 2014 amending Regulation (EC) No 1881/2006 as regards maximum levels of the contaminant citrinin in food supplements based on rice fermented with red yeast Monascus purpureus. (2014). Official Journal of the European Union, 67/3.
9. Priatni, S., Damayanti, S., Saraswaty, V., Ratnaningrum, D., Singgih, M. (2014). The Utilization of Solid Substrates on Monascus Fermentation for Anticholesterol Agent Production. Procedia Chemistry, 9, 34–39. https://doi.org/10.1016/j.proche.2014.05.005
10. Kumari, H. P. M., Naidu, K. A., Vishwanatha, S., Narasimhamurthy, K., Vijayalakshmi, K.G. (2009). Safety evaluation of monascus purpureus red mould rice in albino rats. Food and chemical toxicology, 47(8), 1739–1746. https://doi.org/10.1016/j.fct.2009.04.038
11. Dikshit, R., Tallapragada, P. (2014). Collective effects of stress on optimization of pigment production by monascus purpureus. Chiang mai journal of science, 41(3), 524–530.
12. Food additives code. Ministry of Food and Drug Safety Regulation (2019). [Electronic resource: http://extwprlegs1.fao.org/docs/pdf/kor190402.pdf /Access date 09.09.2020.]
13. Velmurugan, P., Kamala-Kannan, S., Balachandar, V., Lakshmanaperumalsamy, P., Chae, J. — C., Oh, B.-T. (2010). Natural pigment extraction from five filamentous fungi for industrial applications and dyeing of leather. Carbohydrate Polymers, 79(2), 262–268. https://doi.org/10.1016/j.carbpol.2009.07.058
14. Kalinina, I.V., Fatkullin, R.I. (2016). Implementation of effects of ultrasonic cavitation influence as a factor of intensification of extraction of functional elements. Bulletin of the South Ural state university. Series: food and biotechnology, 4(1), 64–70.
15. Rudometova, N. V., Kim, I.S. (2019). Capsaicin extraction from hot pepper of capsicum genus. Processes and food production equipment, 1, 62–73. https://doi.org/10.17586/2310–1164–2019–12–1–62–73
16. Singgih, M., Saraswaty, V., Ratnaningrum, D., Sri Priatni, S., Damayanti, S. (2014). The influence of temperature and ethanol concentration in monacolin K extraction from monascus fermented rice. Procedia Chemistry, 9, 242–247. https://doi.org/10.1016/j.proche.2014.05.029
17. Liang, B., Du, X.-J., Li, P., Sun, C.-C., Wang, S. (2018). Investigation of Citrinin and Pigment Biosynthesis Mechanisms in Monascus purpureus by Transcriptomic. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.01374
18. Maľa, P., Baranová, M., Marcinčáková, D., Nagy, J. (2010). Organoleptic evaluation of poultry meat products with wheat protein — seitan, coloured by microbial natural pigment. Assam university journal of science and technology: biological and environmental sciences, 5, 1–5.
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For citations:
Rudometova N.V., Kim I.S. Research of the physical and chemical properties and methods of Red rice (food colour) determination in sausage products. Theory and practice of meat processing. 2020;5(4):23-28. https://doi.org/10.21323/2414-438X-2020-5-4-23-28
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