Theory and practice of meat processing

Advanced search

Biochemical and histological indicators of blood and m. longissimus dorsi of young bulls of Kazakh white-headed breed of different genotypes by the CAPN1 and GH genes

Full Text:


The use of molecular genetic markers for productivity is one of the modern approaches in breeding beef cattle. The article presents the results of the studies on the influence of genotypes by the calpain (CAPN1) and growth hormone (GH) genes on live weight, fatty acid composition of blood plasma, quantitative and qualitative indicators of meat of young cattle of Kazakh white-headed breed. It was established that animals of the homozygous geno-types СС–CAPN1 and VV-GH had a larger live weight and its average daily gain on the 240th and 365th days. Their superiority over the GG and LL genotypes was 13.8% (P<0.05) and 6.9%; 14.6% (P<0.05) and 6.9%, respectively. The carriers of the desirable alleles in the homozygous state had the sum of unsaturated fatty acids higher by 6.3% and 7.1%, respectively, than the young bulls, in which genotypes they were absent. The predominance of unsaturated fatty acids over saturated fatty acids also determined lower values of lipid me-tabolism direction index (0.92 and 1.00) in the animals with the desirable genotype. Muscle tissue of animals of the СС and VV genotypes was characterized by a higher level of protein, fat by 0.28–2.13 abs. per cent, energy value, and the quantity of muscle fibers with a smaller diameter per unit area compared to the GG and LL genotypes by on average 8.7% and 25.5% (P<0.01). Due to the greater number of interfiber fat inclusions, muscle tissue of the desirable genotypes received higher marbling scores. The obtained data testify to the prospects of replication of animals, which genotype has the desirable alleles for further improvement of traits of meat productivity of Kazakh white-headed breed.

About the Authors

M. I. Selionova
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy
Russian Federation

Marina I. Selionova —  doctor of biological sciences, professor, head of the Department of breeding, genetics and animal biotechnology

127550, Moscow, Timiryazevskaya str., 49

V. R. Plakhtyukova
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy
Russian Federation

Viktoria R. Plakhtyukova —  graduate student

127550, Moscow, Timiryazevskaya str., 49


1. Soloshenko, V.A., Goncharenko, G.M., Dvoryatkin, A.A., Pleshakov, V.A. (2013). Possibilities of using genetic markers in selection of beef cattle to improve the quality of meat indicators. The herald of beef cattle breeding, 1(79), 37–41. (In Russian)

2. Chizhova, L.N., Sharko, G.N., Mihajlenko, A.K. (2016). Genetik markers in the meat animal husbandry. Proceedings of the All-Russian Research Institute of Sheep and Goat Breeding, 9(2), 258–264. (In Russian)

3. Dzhulamanov, K.M., Dubovskova, M. P., Vorozheikin, A. M., Gerasimov, N. P., Kolpakov, V. I. (2016). Slaughter indicators of steers taking into account the selection of parents by genes markers of meat productivity. The herald of beef cattle breeding, 2(94), 26–32. (In Russian)

4. Selionova, M.I., Chizhova, L. N., Dubovskova, M. P., Surzhikova, E. S., Kononova, L. V., Sharko, G. N. (2017). Polymorphism peculiarities of growth hormone (GH), calpain (CAPN1) genes of beef sires. The herald of beef cattle breeding, 2(98), 65–72. (In Russian)

5. Allais, S., Journaux, L., Levéziel, H., Payet-Duprat, N., Raynaud, P., Hocquette, J.F., Lepetit, J., Rousset, S., Denoyelle, C., Bernard-Capel, C., Renand, G. (2011). Effects of polymorphisms in the calpastatin and mu-calpain genes on meat tenderness in 3 French beef breeds. Journal of Animal Science, 89(1), 1–11.–3063

6. Tjulkin, S.V., Ahmetov, T.M., Valiullina, E.F., Vafin R. R. (2012). Polymorphism of genes for somatotropin, prolactin, leptin, and thyroglobulin in stud bulls. Vavilov Journal of Genetics and Breeding, 16(4–2), 1008–1012. (In Russian)

7. Gorlov, I.F., Fedunin, A.A., Randelin, D.A., Sulimova, G.E. (2014). Polymorphism of bGH, RORC and DGAT1 genes in Russian beef cattle breeds. Russian Journal of Genetics, 50(12), 1302– 1307.

8. Surundaeva, L.G. (2016). Comparative analysis of the genetic structure of populations of beef cattle according to polymorphic hormone variants of somatotropin and thyroglobulin. The herald of beef cattle breeding, 4(95), 21–29. (In Russian)

9. Frolov, A.N., Zavialov, O.A., Kharlamov, A.V., Miroshnikov, A.M. (2015). The effect of the genotype steers on the quality of fat and its fatty acid composition. Achievements of Science and Technology of AIC, 29(2), 43–45. (In Russian)

10. Makaev, Sh.A., Taiguzin, R. Sh., Lyapin, O. A, Fomin, A. V. (2019). Genetic characteristic of the kazakh white-headed cattle. News of the Orenburg State Agrarian University, 6(80), 281–285. (in Russian)

11. Hartatik, T., Fathoni, A., Bintara, S., Panjono, I., Eidyobroto, B.P., Agus, A., igs. Budisatria, I., Leroy, P. (2020). Short communication: The genotype of growth hormone gene that affects the birth weight and average daily gain in crossbred beef cattle. Biodiversitas Journal of Biological Diversity, 21(3), 941–945.

12. Costello, S., O’Doherty, E., Troy, D.J., Ernst, C.W., Kim, K.S., Stapleton, P., Sweeney, T., A. M. Mullen, A.M. (2007). Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus dorsi. Meat Science, 75(4), 551–557.

13. Curi, R.A., Chardulo, L.A.L., Giusti, J., Silveira, A.C., Martins, C.L., Oliveira, H.N. (2010). Assessment of GH1, CAPN1 and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus–Bos indicus cross beef cattle. Meat Science, 86(4), 915–920.

14. Tait, R.G., Shackelford, S.D., Wheeler, T. L., King, D.A., Casas, E., Thallman, R.M., Smith, T. P. L., Bennett, G. L. (2014). µ-Calpain, calpastatin, and growth hormone receptor genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in Angus cattle selected to increase minor haplotype and allele frequencies1,2,3. Journal of Animal Science, 92(2), 456–466.–7075

15. Cafe, L. M., McIntyre, B. L., Robinson, D. L., Geesink, G. H., Barendse, W., Pethick, D. W., Thompson, J. M., Greenwood, P. L. (2010). Production and processing studies on calpain-system gene markers for tenderness in Brahman cattle: 2. Objective meat quality1. Journal of Animal Science, 88(9), 3059–3069.–2679

16. Tatsuda, K., Oka, A., Iwamoto, E., Kuroda, Y., Takeshita, H., Kataoka, H., Kouno, S. (2008). Relationship of the bovine growth hormone gene to carcass traits in Japanese black cattle. Journal of animal breeding and genetics, 125(1), 45–49. https://doi. org/10.1111/j.1439–0388.2007.00688.x

17. Khvylya, S.I., Pchelkina, V.A., Burlakova, S.S. (2011). Standardized histological methods for assessing the quality of meat and meat products. Vsyo o myase, 6, 32–35. (In Russian)

18. Budaeva, A.B., Kozub, Yu. A., Ryadinskaya, N. I., Tabakova, M. A. (2019). Histological structure of the longest back muscle of bulls of black-and-white and kazakh white-headed breeds. Bulletin of the Irkutsk State Agricultural Academy, 90, 139–149. (In Russian)

19. Lyudinina, A. Yu., Kochan, T.I., Boiko, Ye. R. (2006). Gas liquid chromatographic determination of tricarbonic cycle acids in human plasma. Russian Clinical Laboratory Diagnostics, 11, 13–14. (In Russian)

20. Kondrakhin, I.P., Arkhipov, A.V., Lechenko, V.I., Talanov, G.A., Frolova, L.A., Novikov, V.E. (2004). Methods of veterinary clinical laboratory diagnostics. Moscow: Kolos. — 520 p. ISBN5–9532– 0165–6 (In Russian)

21. GOST 9793–2016 «Meat and meat products. Methods of moisture determination». Moscow: Standardinform, 2018. — 6 p.

22. GOST 23042–2015 «Meat and meat products. Methods for determining fat». Moscow: Standardinform. 2016. — 9 p.

23. GOST 25011–2017 «Meat and meat products. Methods of protein determination». Moscow: Standardinform, 2018. — 14 p. 24. GOST 19496–2013 «Meat and meat products. Method of histological research». Moscow: Standardinform, 2014. — 10 p.


For citations:

Selionova M.I., Plakhtyukova V.R. Biochemical and histological indicators of blood and m. longissimus dorsi of young bulls of Kazakh white-headed breed of different genotypes by the CAPN1 and GH genes. Theory and practice of meat processing. 2020;5(2):20-25.

Views: 418

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

ISSN 2414-438X (Print)
ISSN 2414-441X (Online)