Physicochemical, microbiological, and organoleptic properties of fermented lamb sausage enhanced by jack bean fortification
https://doi.org/10.21323/2414-438X-2024-9-3-220-226
Abstract
Processing lamb meat into fermented sausages can reduce the risk of spoilage and extend shelf life. Fermented sausages are commonly made using lactic acid bacteria (LAB), resulting in a product that is acidic and less firm. Therefore, it is necessary to add jack bean flour as a binding agent. This study aims to analyze physicochemical, microbiological and organoleptic characteristics of premium IPB lamb fermented sausages with varying proportions of jack bean flour (0% and 30%). The analyses included pH, water activity (aw), total acid, water, ash, fat, crude protein, carbohydrate, total LAB, E. coli, S. aureus, texture profile analysis (TPA), and sensory evaluation. The incorporation of jack bean flour into lamb meat fermented sausages can elevate the pH, springiness, chewiness, crude protein, and carbohydrate content of the fermented sausages. Furthermore, the addition of jack bean flour may reduce the total acid, aw, gumminess, water content, and bacteria (E. coli and S. aureus). The sensory aspects such as color in fermented sausage with addition of jack bean flour were preferred by the panelists. The addition of jack bean flour increased the total unsaturated fatty acids and essential amino acids in fermented sausages. The incorporation of jack bean flour aligns with the Indonesian National Standards for Meat Sausages 3820–2015 concerning moisture, fat, and protein content. This study suggests that incorporating lamb fermented sausage with 30% jack bean flour could result in significant benefits, including increased nutrition, enhanced sensory quality, improved texture, and extended storage life for fresh lamb products.
Keywords
fermented sausage,
jack bean flour,
microbiological,
organoleptic,
physicochemical,
premium IPB lamb meat
Supporting agencies: The authors are grateful to Asian Development Bank and Lembaga Kawasan Sains dan Teknologi, Bogor Agricultural University for their financial support of this research funding with number 13467/IT3.L1/HK.07.00/P/B/2023, and the Directorate of Higher Education of the Ministry of Research, Technology and Higher Education of the Republic of Indonesia who financially supported the research by Competitive Grant 2024.
About the Authors
V. E. Wahyuni
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Indonesia
Indonesia
Venanda Eka Wahyuni, Magister Student, Department of Animal Production and Technology
16680, Bogor
Tel.: +62–813–3156–72–79
I. I. Arief
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Indonesia
Indonesia
Irma Isnafia Arief, Professor, Department of Animal Production and Technology
16680, Bogor
Tel.: +62–812–860–46–13
C. Budima
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Indonesia
Indonesia
Cahyo Budiman, Doctor, Department of Animal Production and Technology
16680, Bogor
Tel.: +62–878–3091–82–02
A. Gunawan
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Indonesia
Indonesia
Asep Gunawan, Professor, Department of Animal Production and Technology
16680, Bogor
Tel.: +62–812–9705–75–56
References
1. Komariah, Rahayu, S., Sarjito. (2009). Physical charactertal rats. Jurnal Ilmu Produksi dan Teknologi Peternakan, 2(1), istics of beef, buffalo and lamb meat on different postmortem periods. Buletin Peternakan, 33(3), 183–189. https://doi.org/10.21059/buletinpeternak.v33i3.115
2. Sujarwanta, R.0., Afidah, U., Suryanto, E., Rusman., Triyannanto E., Hoffman, L.C. (2024). Review: Goat and sheep meat production in Indonesia. Sustainability, 16(11), Article 4448. https://doi.org/10.3390/su16114448
3. Febriani, C.D., Larasati, D., Sampurno, A. (2020). The effect of immersion time in liquid nitrogen on physical and chemical properties of meatballs during frozen storage. Jurnal Teknologi Pangan dan Hasil Pertanian, 12(2), 15–22. http://doi.org/10.26623/jtphp.v15i2.2656 (In Indonesian)
4. Bolla, N.E., Mahaputra, I.M., Robi, I.M., Juniartini, W.S., Nazara, A.L., Swacita, I.B.N. (2023). Evaluation of the quality of meat and processed meat product from Kumbasari traditional market and Cokroaminoto market. Denpasar city, Bali. Buletin Veteriner Udayana, 15(2), 222–241. http://doi.org/10.24843/bulvet.2023.v15.i02.p09 (In Indonesian)
5. Ernaningsih, D., Arief, I.I., Taufik, E. (2015). The influence of granting fermented sausage on the growth of the experimen- 207–212. https://doi.org/10.29244/JIPTHP.2.1.207-212 (In Indonesian)
6. Putri, A.L.O., Kusdiyantini, E. (2018). Isolation and identification of lactic acid bacteria from fish-based fermented foods (Inasua) from Maluku-Indonesia. Jurnal Biologi Tropika, 1(2), 6–12. http://doi.org/10.14710/jbt.1.2.6-12 (In Indonesian)
7. Suharto, E. L. S., Arief, I.I., Taufik, E. (2016). Quality and antioxidant activity of yogurt supplemented with roselle during cold storage. Media Peternakan, 39(2), 82–89. http://doi.org/10.5398/medpet.2016.39.2.82
8. Arief, I.I., Budiman, C., Hanifah, R., Soenarno, M.S. (2016). Antihypertensive potency of goat milk yoghurt supplemented by probiotic and roselle extract. International Journal of Sciences: Basic and Applied Research (IJSBAR), 30(4), 207– 214. https://doi.org/10.5398/tasj.2018.41.3.191
9. Wihansyah, R.R.S., Arief, I.I., Batubara, I. (2018). Anti-diabetic potency and characteristics of probiotic goat-milk yogurt supplemented with roselle extract during cold storage. Tropical Animal Science Journal, 41(3), 191–199. http://doi.org/10.5398/tasj.2018.41.3.191
10. Beltrán-Barrientos, L.M., Hernandez, A.M., Torres-Llanez, M.J., González-Córdova, A.F., Vallejo-Córdoba, B. (2016). Invited review: Fermented milk as antihypertensive functional food. Journal of Dairy Science, 99(6), 4099–4110. https://doi.org/10.3168/jds.2015-10054
11. Nisa, A.K., Wardani, A.K. (2015). The effect of smoking time and fermentation on the fermented sausage catfish (Clarias gariepinus) quality. Jurnal Pangan dan Agroindustri, 4(1), 367–376. (In Indonesian)
12. Nursyam, H. (2011). Tuna fish (Thunnus albacares) fermented sausage processing using Lactobacillus plantarum starter culture against pH, total acid, n-total, and n-amino. Jurnal Ilmiah Perikanan dan Kelautan, 3(2), 221–228. https://doi.org/10.20473/jipk.v3i2.11609 (In Indonesian)
13. Usman, U., Rahim, I., Ambar, A.A. (2013). Analysis growth and production of word jack bean (Canavalia ensiformis) in various concentration of organic liquid fertilizer and pruning. Jurnal Galung Tropika, 2(2), 85–96. https://doi.org/10.31850/jgt.v2i2.46 (In Indonesian)
14. Suryaningrum, R., Kusuma, P.S.W. (2013). Optimization of white sword bean (Canavalia ensiformis L) dosage as a raw material for yogurt production. STIGMA, 6(2), 7–12. https://doi.org/10.36456/stigma.vol6.no02.a521 (In Indonesian)
15. Toro, A.N., Roosmarinto, Rahayu, M. (2014). The effect of soaking duration of velvet bean (Mucuna pruriens) in lime water (Ca(OH)₂) on cyanide acid (HCN) content. Jurnal Teknologi Laboratorium, 3, 52–56. (In Indonesian)
16. Nissar, J., Ahad, T., Naik, H.R., Hussain, S.Z. (2017). A review phytic acid: As antinutrient or nutraceutical. Journal of Pharmacognosy and Phytochemistry, 6, 1554–1560.
17. Ariyantoro, A.R., Rachmawati, D., Ikarini, I. (2016). Physicochemical characteristic of modified jack bean (Canavalia ensiformis) flour with various lactic acid concentration and soaking time. Jurnal Agritech, 36(1), 1–5. https://doi. org/10.22146/agritech.10675 (In Indonesian)
18. Arief, I.I., Budiman, C., Jenie, B.S.L., Andreas, E., Yuneni, A. (2015). Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 display bactericidal activity against Staphylococcus aureus. Beneficial Microbes, 6(4), 603–613. https://doi.org/10.3920/bm2014.0064
19. Arief, I.I., Wulandari, Z., Aditia, E.L., Baihaqi, M., Noraimah, Hendrawan. (2014). Physicochemical and Microbiological Properties of Fermented Lamb Sausages using Probiotic Lactobacillus plantarum IIA-2C12 as Starter Culture. Procedia Environmental Sciences, 20(2014), 352–356. https://doi.org/10.1016/j.proenv.2014.03.044
20. AOAC (2005). Official Method of Analysis. 18th Ed. Maryland (US): AOAC International.
21. Angelia, I.O. (2017). pH content, total acidified acid, dissolved solids and vitamin C in some horticultural commodities. Journal of Agritech Science (JASc), 1(2), 68–74. https://doi.org/10.30869/jasc.v1i2.133 (In Indonesian)
22. Osthoff, G.A., Hugo, Venter, H. (2002). Study of the changes in protein fractions and amino acids of an unfermented South African dried sausage. Journal Food Technology Afrika, 7(3), 101–108. http://doi.org/10.4314/jfta.v7i3.19241
23. AOAC (2012). Official Method of Analysis. 22nd Ed. New York (US): AOAC International.
24. Samuel, K.S., Peerkham, N. (2020). Pearl millet protein bar: Nutritional, organoleptic, textural characteristic, and in vitro protein and starch digestibility. Journal of Food Science and Technology, 57(9), 3467–3473. https://doi.org/10.1007/s13197020-04381-x
25. Badan Standarisasi Nasional. (2008). SNI 2897–2008. Methods for testing microbial contamination in meat, eggs, milk, and their products. Jakarta: Badan Standardisasi Nasional. (In Indonesian)
26. Badan Standarisasi Nasional. (2006). SNI 01–2346–2006. Guidelines for organoleptic and/or sensory testing. Jakarta: Badan Standardisasi Nasional. (In Indonesian)
27. Setiarto, R.H.B., Widhyastuti, N., Fairuz, I. (2017). Effect of lactic acid bacteria starter and the fortification of modified taro flour on the quality of synbiotic yogurt. Jurnal Riset Teknologi Industri, 11(1), 18–30. http://doi.org/10.26578/jrti.v11i1.2179 (In Indonesian)
28. Rini, A.W., Handajani, S., Affandi, D. R. (2009). The effect of koro glinding (Phaseolus lunatus) flour addition on chemical and organoleptic properties of wheat flour-made wet noodle substituted with purple sweet potato flour. Biofarmasi Journal of Natural Product Biochemistry, 7(1), 31–41. (In Indonesian)
29. Widiantara, T., Cahyadi, W., Razak, I.L. (2017). Utilization of jack bean (Canavalia ensiformis L) for tofu production based on different coagulant concentrations. Pasundan Food Technology Journal, 4(3), 182–190. http://doi.org/10.23969/pftj.v4i3.644 (In Indonesian)
30. Akinyemi, F.A., Orishadipe, A.T., Oluwa, O.E., Aladesanmi, O.A. (2020). Physicochemical properties and functional characteristics of Jack beans (Canavalia ensiformis) starch. World Journal of Biology Pharmacy and Health Sciences, 3(2), 12–22. https://doi.org/10.30574/wjbphs.2020.3.2.0018
31. Windrati, S.W., Nafi’, N., Augustine, P.D. (2010). Nutritional properties of jack bean PRF (Canavalia ensiformis L.). Jurnal Agroteknologi, 4, 18–26. (In Indonesian)
32. Park, W., Kim, J.-H., Ju, M.-G., Hong, G.-E., Yeon, S.-J., Seo, H. G. et al. (2017). Enhancing quality characteristics of salami sausages formulated with whole buckwheat flour during storage. Journal of Food Science and Technology, 54(2), 326– 332. https://doi.org/10.1007/s13197-016-2465-8
33. Setyaningsih, D., Apriyantono, A., Sari, M.P. (2010). Sensory analysis for the food and agro industry. Bogor: IPB Press, 2010. (In Indonesian)
34. Abitogun, A.S., Olasehinde, E.F. (2012). Nutritional evaluation of seed and characterization of crude jack bean (Canavalia ensiformis) OIL. IOSR Journal of Applied Chemistry, 1(6), 36–40. http://doi.org/10.9790/5736-0163640
35. Nafi, A., Windrati, W.S., Pamungkas, A., Subagio, A. (2013). Protein rich flour from hyacinth bean as functional food ingredient with low glycemic index. Jurnal Teknologi dan Industri Pangan, 24(1), 1–6. https://doi.org/10.6066/jtip.2013.24.1.1 (In Indonesian)
36. Nousiainen, J., Ahvenjarvi, S., Rinne, M., Hellamaki, M., Huhtanen, P. (2004). Prediction of indigestible cell wall fraction of grass silage by near infrared reflectance spectroscopy. Animal Feed Science and Technology, 115(3), 295–311. https://doi.org/10.1016/j.anifeedsci.2004.03.004
37. More, K., Tayade, S., Gawande, P., Manik, S., Shalke, D. (2022). Antioxidant and antimicrobial potential of Canavalia gladiate (Jacq.) DC. leaves and seeds: GC–MS based metabolic profiling. Indian Journal of Natural Products and Resources, 13(2), 163–169. http://doi.org/10.56042/ijnpr.v13i2.47499
38. Arief, I.I., Jenie, B.S.L., Astawan, M., Witarto, A.B. (2010). The effectivities of probiotic Lactobacillus plantarum 2C12 and Lactobacillus acidophilus 2B4 as antidiarrhea on rats. Media Peternakan, 33(3), 137–143. https://doi.org/10.5398/medpet.2010.33.3.137 (In Indonesian)
39. Arief, I.I., Jenie, B.S.L, Suryati, T., Ayuningtyas, G., Fuziawan, A. (2012). Antimicrobial activity of bacteriocin from indigenous Lactobacillus plantarum 2C12 and its application on beef meatball as biopreservative. Journal of the Indonesian Tropical Animal Agriculture, 37, 90–96. http://doi.org/10.14710/jitaa.37.2.90-96
40. Gunawan, A., Listyarini, K., Harahap, R.S., Jakaria, Roosita, K., Sumantri, C. et al. (2021). Hepatic transcriptome analysis identifies genes, polymorphisms and pathways involved in the fatty acids metabolism in sheep. PLoS One, 16(12), Article e0260514. https://doi.org/10.1371/journal.pone.0260514
41. Khasbullah, F., Mangiring, W., Krisnarini, Kurniawati, N. (2024). Antioxidant and lactic acid bacteria activities of kimchi pakcoy due to salt concentration and fermentation time. Jurnal Agroindustri, 14(1), 77–86. https://doi.org/10.31186/jagroindustri.14.1.77-86 (In Indonesian)
42. Kinteki, G.A., Rizqiati, H., Hintono, A. (2018). Effect on the fermentation duration of goat milk kefir toward hedonic quality, total lactic acid bakteria (BAL), total khamir, and pH. Jurnal Teknologi Pangan, 3(1), 42–50. https://doi.org/10.14710/jtp.2019.20685 (In Indonesian)
43. Sridhar, K.R., Sharma, B. (2021). Bioactive compounds of Jack Beans (Canavalia Species). Chapter in a book: Bioactive Compounds in Underutilized Vegetables and Legumes. Reference Series in Phytochemistry. Springer, Cham, 2021. https://doi.org/10.1007/978-3-030-57415-4_26
44. Carballo, J. (2021). Sausages: Nutrition, safety, processing and quality improvement. Foods, 10(4), Article 890. https://doi.org/10.3390/foods10040890
45. Kanetro, B., Riyanto, M., Pujimulyani, D., Huda, N. (2021). Improvement of functional properties of jack bean (Canavalia ensiformis) flour by germination and its relation to amino acids profile. Journal Current Research in Nutrition and Food Science, 9(3), 812–822. http://doi.org/10.12944/CRNFSJ.9.3.09
46. Rose, A.J. (2019). Amino acid nutrition and metabolism in health and disease. Nutrients, 11, Article 2623. https://doi.org/10.3390/nu11112623
47. Wu, G. (2010). Functional amino acids in growth, reproduction, and health. Advances in Nutrition, 1, 31–37. https://doi.org/10.3945/an.110.1008
48. Arianto, A., Nahong, B., Nurhaedah. (2014). Analysis of acid content of cyanide (HCN) at koro sword beans (Canavalia ensiformis) using different old immersion NaCL. Jurnal Galang Tropika, 3(3), 186–191. (In Indonesian)
49. Hustiany, R. (2016). Maillard reaction: Flavor and color formation in food products. Banjarmasin: Lambung Mangkurat University Press, 2016. (In Indonesian)
50. Daun, H. (1979). Interaction of wood smoke components and foods. Food Technology, (32), 66–71. https://doi.org/10.1021/jf60184a018
51. Deng, Y., Wang, Y., Yue, J., Liu, Z., Zheng, Y., Qian, B. et al. (2014). Thermal behavior, microstructure and protein quality of squid fillets dried by far-infrared assisted heat pump drying. Food Control, 36(1), 102–110. https://doi.org/10.1016/j.foodcont.2013.08.006
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For citations:
Wahyuni V.E., Arief I.I., Budima C., Gunawan A. Physicochemical, microbiological, and organoleptic properties of fermented lamb sausage enhanced by jack bean fortification. Theory and practice of meat processing. 2024;9(3):220-226. https://doi.org/10.21323/2414-438X-2024-9-3-220-226
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