Development of a premix based on micellar casein for fortification of meat systems with vitamin A
https://doi.org/10.21323/2414-438X-2024-9-1-65-74
Abstract
A novel approach to the protection of unstable fat-soluble vitamins, using retinol as an example, is presented in this work. This method is based on introducing vitamin A molecules into casein micelles. Protective properties of micellar casein towards different forms of retinol (native vitamin and palmitic acid ester) in vitro and in emulsion-type meat products are investigated. A technology of the introduction using micellar casein concentrate (MCC) has been developed. Conditions similar to those in which vitamin molecules can be in meat emulsions during heat treatment are simulated in vitro. The optimal time of “encapsulation” (2 hours) and the need for additional surfactant (tween-80) are identified. The use of the casein micelles protection made it possible to increase the number of retinol molecules that did not undergo decomposition under model conditions (in vitro) from ~30% to ~80%. Using the vitamin premix the degree of degradation of vitamin molecules does not exceed 4% after heat treatment. Data received allowed us to determine the efficiency of the protection properties of casein micelles for unstable vitamin A molecules.
About the Authors
N. A. Dzhangiryan
Department of Food Technologies and Engineering, Faculty of Food Engineering and Biotechnology, North-Caucasus Federal University
Russian Federation
Russian Federation
Narek A. Dzhangiryan, PhD student
1, Pushkin Street, 355017, Stavropo
Tel.: +7–918–574–86–56
V. I. Shipulin
Department of Food Technologies and Engineering, Faculty of Food Engineering and Biotechnology, North-Caucasus Federal University
Russian Federation
Russian Federation
Valentin I. Shipulin, Doctor of Technical Sciences, Professor
1, Pushkin Street, 355017, Stavropol
Tel.: +7–962–400–55–40
D. N. Volodin
“DMP” LLC
Russian Federation
Russian Federation
Dmitry N. Volodin, Candidate of Technical Science, Director
160, Dzerzhinsky Street, 355003, Stavropol
Tel.: +7–865–299–89–88
References
1. Kodentsova, V. M., Beketova, N. A., Nikityuk, D. B., Tutelyan, V. A. (2018). Characteristics of vitamin provision in the adult population of the Russian Federation. The Russian Journal of Preventive Medicine, 21(4), 32–37. https://doi.org/10.17116/profmed201821432 (In Russian)
2. Dzhangiryan, N. A., Shipulin, V. I. (April 4–9, 2022). Application of milk proteins in the meat industry: Achievements and prospects. Biodiversity, bioresources, biotechnology and health of the population of the north caucasus region. Materials of the IX (66th) Annual Scientific and Practical Conference. Stavropol, Russia, 2022. (In Russian)
3. Shipulin, V. I., Dzhangiryan, N. A. (2023). Properties of micellar casein and application in food industry. Modern Science and Innovations, 1, 56–64.
4. Morr, C. V. (1979). Utilization of milk proteins as starting materials for other foodstuffs. Journal of Dairy Research, 46(2), 369–376. https://doi.org/10.1017/S0022029900017337
5. Singh, H. (2011). Milk Protein Products: Functional Properties of Milk Proteins. Chapter in a book: Encyclopedia of Dairy Sciences. Academic Press, 2011. https://doi.org/10.1016/B978-0-12-374407-4.00352-6
6. Horne, D. S. (2006). Casein micelle structure: Models and muddles. Current Opinion in Colloid and Interface Science, 11(2–3), 148–153. https://doi.org/10.1016/J.COCIS.2005.11.004
7. Hammam, A. R. A., Martínez-Monteagudo, S. I., Metzger, L. E. (2021). Progress in micellar casein concentrate: Production and applications. Comprehensive Reviews in Food Science and Food Safety, 20(5), 4426–4449. https://doi.org/10.1111/1541-4337.12795
8. Penalva, R., Esparza, I., Agüeros, M., Gonzalez-Navarro, C. J., Gonzalez-Ferrero, C., Irache, J. M. (2015). Casein nanoparticles as carriers for the oral delivery of folic acid. Food Hydrocolloids, 44, 399–406. https://doi.org/10.1016/j.foodhyd.2014.10.004
9. Morçöl, T., Nagappan, P., Nerenbaum, L., Mitchell, A., Bell, S. J. D. (2004). Calcium phosphate-PEG-insulin-casein (CAPIC) particles as oral delivery systems for insulin. International Journal of Pharmaceutics, 277(1–2), 91–97. https://doi.org/10.1016/j.ijpharm.2003.07.015
10. Esmaili, M., Ghaffari, S. M., Moosavi-Movahedi, Z., Atri, M. S., Sharifizadeh, A., Farhadi, M. et al. (2011). Beta casein-micelle as a nano vehicle for solubility enhancement of curcumin; food industry application. LWT-Food Science and Technology, 44(10), 2166–2172. https://doi.org/10.1016/j.lwt.2011.05.023
11. Menéndez-Aguirre, O., Kessler, A., Stuetz, W., Grune, T., Weiss, J., Hinrichs, J. (2014). Increased loading of vitamin D2 in reassembled casein micelles with temperature-modulated high pressure treatment. Food Research International, 64, 74– 80. https://doi.org/10.1016/j.foodres.2014.06.010
12. Huang, J., Shu, Q., Wang, L., Wu, H., Wang, A. Y., Mao, H. (2015). Layer-by-layer assembled milk protein coated magnetic nanoparticle enabled oral drug delivery with high stability in stomach and enzyme-responsive release in small intestine. Biomaterials, 39, 105–113. https://doi.org/10.1016/j.biomaterials.2014.10.059
13. Ranadheera, C. S., Liyanaarachchi, W. S., Chandrapala, J., Dissanayake, M., Vasiljevic, T. (2016). Utilizing unique properties of caseins and the casein micelle for delivery of sensitive food ingredients and bioactives. Trends in Food Science and Technology, 57(Part A), 178–187. https://doi.org/10.1016/j.tifs.2016.10.005
14. Livney, Y. D. (2010). Milk proteins as vehicles for bioactives. Current Opinion in Colloid and Interface Science, 15(1–2), 73– 83. https://doi.org/10.1016/j.cocis.2009.11.002
15. Semo, E., Kesselman, E., Danino, D., Livney, Y. (2007). Casein micelle as a natural nano-capsular vehicle for nutraceuticals. Food Hydrocolloids, 21(5–6), 936–942. https://doi.org/10.1016/j.foodhyd.2006.09.006
16. Levinson, Y., Ish-Shalom, S., Segal, E., Livney, Y. D. (2016). Bioavailability, rheology and sensory evaluation of fat-free yogurt enriched with VD3 encapsulated in re-assembled casein micelles. Food and Function, 7(3), 1477–1482. https://doi.org/10.1039/c5fo01111f
17. Haham, M., Ish-Shalom, S., Nodelman, M., Duek, I., Segal, E., Kustanovich, M., Livney, Y. D. (2012). Stability and bioavailability of vitamin D nanoencapsulated in casein micelles. Food and Function, 3(7), 737–744. https://doi.org/10.1039/c2fo10249h
18. Sáiz-Abajo, M.-J., González-Ferrero, C., Moreno-Ruiz, A., Romo-Hualde, A., González-Navarro, C. J. (2013). Thermal protection of β-carotene in re-assembled casein micelles during different processing technologies applied in food industry. Food Chemistry, 138(2–3), 1581–1587. https://doi.org/10.1016/j.foodchem.2012.11.016
19. Jarunglumlert, T., Nakagawa, K., Adachi, S. (2015). Influence of aggregate structure of casein on the encapsulation efficiency of β-carotene entrapped via hydrophobic interaction. Food Structure, 5, 42–50. https://doi.org/10.1016/j.foostr.2015.05.001
20. Mohan, M. S., Jurat-Fuentes, J. L., Harte, F. (2013). Binding of vitamin A by casein micelles in commercial skim milk. Journal of Dairy Science, 96(2), 790–798. https://doi.org/10.3168/ jds.2012-5777
21. Li, Y., Li, Y., Yuan, D., Wang, Y., Li, M., Zhang, L. (2020). The effect of caseins on the stability and whipping properties of recombined dairy creams. International Dairy Journal, 105, Article 104658. https://doi.org/10.1016/j.idairyj.2020.104658
22. Karam, M. C., Gaiani, C., Hosri, C., Burgain, J., Scher, J. (2013). Effect of dairy powders fortification on yogurt textural and sensorial properties: A review. Journal of Dairy Research, 80(4), 400–409. https://doi.org/10.1017/S0022029913000514
23. Amelia, I., Drake, M., Nelson, B., Barbano, D. M. (2013). A new method for the production of low-fat Cheddar cheese. Journal of Dairy Science, 96(8), 4870–4884. https://doi.org/10.3168/jds.2012-6542
24. Ozturk-Kerimoglu, B., Urgu-Ozturk, M., Serdaroglu, M., Koca, N. (2022). Chemical, technological, instrumental, microstructural, oxidative and sensory properties of emulsified sausages formulated with microparticulated whey protein to substitute animal fat. Meat Science, 184, Article 108672. https://doi.org/10.1016/j.meatsci.2021.108672
25. Barbut, S. (2006). Effects of caseinate, whey and milk powders on the texture and microstructure of emulsified chicken meat batters. LWT — Food Science and Technology, 39(6), 660–664. https://doi.org/10.1016/J.LWT.2005.03.017
26. Barbut, S. (2010). Effects of milk powder and its components on texture, yield, and color of a lean poultry meat model system. Poultry Science, 89(6), 1320–1324. https://doi.org/10.3382/ps.2009-00506
27. Hongsprabhas, P., Barbut, S. (1999). Effect of pre-heated whey protein level and salt on texture development of poultry meat batters. Food Research International, 32(2), 145–149. https://doi.org/10.1016/S0963-9969(99)00065-4
28. Youssef, M. K., Barbut, S. (2011). Effects of two types of soy protein isolates, native and preheated whey protein isolates on emulsified meat batters prepared at different protein levels. Meat Science, 87(1), 54–60. https://doi.org/10.1016/j.meatsci.2010.09.002
29. Andrès, S., Zaritzky, N., Califano, A. (2006). The effect of whey protein concentrates and hydrocolloids on the texture and colour characteristics of chicken sausages. International Journal of Food Science and Technology, 41(8), 954–961. https://doi.org/10.1111/j.1365-2621.2005.01152.x
30. Statsenko, E.N., Shipulin, V.I., Dzhangiryan, N.A., Volodin, D. N. Method for production of white sausages from poultry meat. Patent RF, no. 2801108, 2022. (In Russian)
31. Goff, H. D., Liboff, M., Jordan, W. K., Kinsella, J. E. (1987). The effects of polysorbate 80 on the fat emulsion in ice cream mix: Evidence from transmission electron microscopy studies. Food Microstrusture, 6(2), 193–198.
32. CovInfo. (2011). Food and drug administration, department of health and human services. Section: Polysorbate 80. Chapter in Code of Federal Regulations. Retrieved from https://www.govinfo.gov/app/details/CFR-2011-title21-vol3/CFR-2011-title21-vol3-sec172–840. Accessed August 7, 2023.
33. Choi, Y. H., Kim, H. K., Wilson, E. G., Erkelens, C., Trijzelaar, B., Verpoorte, R. (2004). Quantitative analysis of retinol and retinol palmitate in vitamin tablets using 1 H-nuclear magnetic resonance spectroscopy. Analytica Chimica Acta, 512(1), 141–147. https://doi.org/10.1016/j.aca.2004.02.024
34. Yeh, E. B., Barbano, D. M., Drake, M. (2017). Vitamin fortification of fluid milk. Journal of Food Science, 82(4), 856–864. https://doi.org/10.1111/1750-3841.13648
35. Lovkis, Z.V., Pochitskaja, I.M., Labazava, I.E., Komarova, N.V. (2020). Monitoring of meat products presented in the consumer market of Minsk. Food Industry: Science and Technologies, 13(3), 91–110. (In Russian) 36. Tolkunova, N.N, (2004). Mineral and vitamin composition of new types of boiled sausages. Food Industry, 10, 86. (In Russian)
Review
For citations:
Dzhangiryan N.A., Shipulin V.I., Volodin D.N. Development of a premix based on micellar casein for fortification of meat systems with vitamin A. Theory and practice of meat processing. 2024;9(1):65-74. https://doi.org/10.21323/2414-438X-2024-9-1-65-74
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