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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">meat</journal-id><journal-title-group><journal-title xml:lang="en">Theory and practice of meat processing</journal-title><trans-title-group xml:lang="ru"><trans-title>Теория и практика переработки мяса</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2414-438X</issn><issn pub-type="epub">2414-441X</issn><publisher><publisher-name>ФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2414-438X-2018-3-3-36-55</article-id><article-id custom-type="elpub" pub-id-type="custom">meat-96</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>METHODOLOGICAL ASPECTS OF IDENTIFICATION OF TISSUE-SPECIFIC PROTEINS AND PEPTIDES FORMING  THE CORRECTIVE PROPERTIES OF INNOVATIVE MEAT PRODUCTS</article-title><trans-title-group xml:lang="ru"><trans-title>МЕТОДОЛОГИЧЕСКИЕ АСПЕКТЫ ИДЕНТИФИКАЦИИ ТКАНЕСПЕЦИФИЧНЫХ БЕЛКОВ И ПЕПТИДОВ, ФОРМИРУЮЩИХ КОРРИГИРУЮЩИЕ СВОЙСТВА ИННОВАЦИОННЫХ МЯСНЫХ ПРОДУКТОВ</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вострикова</surname><given-names>Н. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Vostrikova</surname><given-names>Natal’ya L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>кандидат технических наук, заведующий лабораторией «Научно-методические работы, биологические и аналитические исследования»</p><p>109316, г. Москва, ул. Талалихина, 26Тел.: +7–495–676–79–81</p></bio><bio xml:lang="en"><p>candidate of technical sciences, head of laboratory «Scientific and methodical work, biological and analytical research»</p><p>109316, Moscow, Talalikhina str., 26Tel.: +7–495–676–79–81</p></bio><email xlink:type="simple">n.vostrikova@fncps.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чернуха</surname><given-names>И. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Chernukha</surname><given-names>Irina M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор технических наук, профессор, главный научный сотрудник Экспериментальной клиники-лаборатории биологически активных веществ животного происхождения, Всероссийский научно-исследовательский институт мясной промышленности имени В.М. Горбатова</p><p>109316, г. Москва, ул. Талалихина,26Тел.: +7–495–676–63–21</p></bio><bio xml:lang="en"><p>doctor of technical sciences, professor, leading research scientist of Experimental clinic — laboratory «Biologically active substances of an animal origin»</p><p>109316, Moscow, ul. Talalikhina, 26Tel.: +7–495–676–63–21</p></bio><email xlink:type="simple">imcher@inbox.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Хвостов</surname><given-names>Д. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Khvostov</surname><given-names>Daniil V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>младший научный сотрудник лаборатории биоаналитических исследований, Научный центр биомедицинских технологий Федерального медико-биологического агентства</p><p>143442, Московская область, Красногорский район, пос. Светлые Горы, владение 1 </p><p>Тел. +7–495–561–5264</p></bio><bio xml:lang="en"><p>junior researcher of the Laboratory of Bioanalytical Research</p><p>143442, Moscow region, Krasnogorsk district, Svetlye gory village, 1Теl. +7–495–561–5264</p></bio><email xlink:type="simple">scbmt@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Федеральный научный центр пищевых систем им. В.М. Горбатова РАН, Москва</institution><country>Россия</country></aff><aff xml:lang="en"><institution>V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Федеральный научный центр пищевых систем им. В.М. Горбатова РАН, Москва; Научный центр биомедицинских технологий Федерального медико-биологического агентства России, Московская область</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Scientific Center of Biomedical Technology of the Federal Medical-Biological Agency of Russia, Moscow region</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>27</day><month>09</month><year>2018</year></pub-date><volume>3</volume><issue>3</issue><fpage>36</fpage><lpage>55</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Vostrikova N.L., Chernukha I.M., Khvostov D.V., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Вострикова Н.Л., Чернуха И.М., Хвостов Д.В.</copyright-holder><copyright-holder xml:lang="en">Vostrikova N.L., Chernukha I.M., Khvostov D.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.meatjournal.ru/jour/article/view/96">https://www.meatjournal.ru/jour/article/view/96</self-uri><abstract><p>One of the ways to address the food quality issues facing the industry is the development of standardized and certified methods related to the conduct of in-depth studies of biochemical indicators of quality and safety of meat and meat products. The world laboratory practice in the field of food quality and safety shows a constant expansion of the list of controlled indicators of food raw materials and products. An important feature of the modern period in the development of biomedical and biotechnological research is the introduction of a whole complex of postgenomic technologies, which are based on a systematic approach to the study of the functioning of the mammalian proteome in various physiological and pathological conditions, including the formation and development of alimentary-dependent pathologies. In this regard, the problem of multilateral study of food products, in particular their identification, is the most relevant, because the modern technology of their production has undergone significant changes and requires the development of “gentle “ processing modes. They concern raw materials and auxiliary materials used at all stages of production. This and new technologies of production of protein products from plant raw materials, as well as the introduction of food raw materials and food additives of artificial origin and the excess introduction of additives of plant and animal origin can cause falsification of products, as well as affect the health of the consumer. Food quality assessment includes the control of components of finished products. It is most difficult to determine the proportion of muscle protein in multi-component meat products that have undergone heat treatment. Therefore, in practice, when assessing the quality of food products, there is a need to identify its real composition in accordance with the declared normative documents. Currently, a promising area of research in the field of determining the composition of finished food is the selection of biomarkers of various components. Therefore, it is important to develop a methodology for the identification of biochemical changes in food raw materials under the influence of technological factors using modern research methods. This paper provides an overview of the protein and peptide analysis methodology, including the latest technologies that are becoming increasingly important.</p></abstract><trans-abstract xml:lang="ru"><p>Одним из направлений решения стоящих перед отраслью вопросов качества питания является разработка стандартизированных и аттестованных методик, связанных с проведением глубоких исследований биохимических показателей качества и безопасности мяса и мясных продуктов. Мировая лабораторная практика в области качества и безопасности продуктов питания свидетельствуют о постоянном расширении списка контролируемых показателей пищевого сырья и продукции. Важной особенностью современного периода в развитии биомедицинских и биотехнологических исследований является внедрение целого комплекса постгеномных технологий, в основе которых лежит системный подход к изучению особенностей функционирования протеома млекопитающего при различных физиологических и патологических состояниях, в том числе при формировании и развитии алиментарно-зависимых патологий. В связи с этим проблема многостороннего изучения пищевых продуктов, в частности их идентификация, является наиболее актуальной, поскольку современная технология их производства претерпела существенные изменения и требует разработки «щадящих» режимов обработки. Они касаются исходного сырья и вспомогательных материалов, используемых на всех этапах производства продукции. Это и новые технологии производства белковых продуктов из растительного сырья, а  также введение в  продовольственное сырье и  продукты питания пищевых добавок искусственного происхождения и избыточное внесение добавок растительного и животного может стать причиной фальсификации продуктов, а также сказаться на здоровье потребителя. Оценка качества пищевых продуктов включает контроль компонентов готовых изделий. Наиболее сложно определить долю мышечнотканного белка в многокомпонентных мясных продуктах, прошедших термическую обработку. Поэтому в практике при оценке качества пищевых продуктов возникает необходимость идентификации его реального состава в соответствии с декларированным нормативным документам. В настоящее время перспективным направлением научных исследований в области определения состава готовых пищевых продуктов является выделение биомаркеров различных компонентов. В связи с этим актуальным является разработка методологии идентификации биохимических изменений пищевого сырья под действием технологических факторов с использованием современных методов исследования. В данной работе представлен обзор методологии анализа белков и пептидов, включающий последние технологии, которые приобретают всю большую актуальность.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>протеомика</kwd><kwd>двумерный электрофорез</kwd><kwd>биомаркеры</kwd><kwd>масс-спектрометрия</kwd></kwd-group><kwd-group xml:lang="en"><kwd>proteomics</kwd><kwd>two-dimensional electrophoresis</kwd><kwd>biomarkers</kwd><kwd>mass spectrometry</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование выполнено за счет гранта Российского научного фонда (проект № 16–16–10073).</funding-statement><funding-statement xml:lang="en">The study was funded by the grant of the Russian Scientific Foundation (project No. 16–16–10073).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts K., Walter P. 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