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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">meat</journal-id><journal-title-group><journal-title xml:lang="en">Theory and practice of meat processing</journal-title><trans-title-group xml:lang="ru"><trans-title>Теория и практика переработки мяса</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2414-438X</issn><issn pub-type="epub">2414-441X</issn><publisher><publisher-name>ФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2414-438X-2024-9-3-212-219</article-id><article-id custom-type="elpub" pub-id-type="custom">meat-375</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Improving the functional and technological properties of minced pork using a proteolytic enzyme</article-title><trans-title-group xml:lang="ru"><trans-title></trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="western" xml:lang="en"><surname>Semenova</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="en"><p>26, Talalikhin str., 109316, Moscow</p><p>Tel.: +7–495–676–95–11 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="western" xml:lang="en"><surname>Kuznetsova</surname><given-names>T. G.</given-names></name></name-alternatives><bio xml:lang="en"><p>26, Talalikhin str., 109316, Moscow</p><p>Tel.: +7–495–676–95–11 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="western" xml:lang="en"><surname>Seliverstova</surname><given-names>O. A.</given-names></name></name-alternatives><bio xml:lang="en"><p>26, Talalikhin str., 109316, Moscow</p><p>Tel.: +7–495–676–95–11 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="western" xml:lang="en"><surname>Salikova</surname><given-names>M. N.</given-names></name></name-alternatives><bio xml:lang="en"><p>26, Talalikhin str., 109316, Moscow</p><p>Tel.: +7–495–676–95–11 </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8372-5490</contrib-id><name-alternatives><name name-style="western" xml:lang="en"><surname>Spirina</surname><given-names>M. E.</given-names></name></name-alternatives><bio xml:lang="en"><p>Marina N. Salikova, Graduate Student, Applied and Technological Development Department</p><p>26, Talalikhin str., 109316, Moscow</p><p>Tel.: +7–495–676–95–11 </p></bio><email xlink:type="simple">mav_rik@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5897-2775</contrib-id><name-alternatives><name name-style="western" xml:lang="en"><surname>Bukhteeva</surname><given-names>Yu. M.</given-names></name></name-alternatives><bio xml:lang="en"><p>Yulia M. Bukhteeva, Candidate of Technical Sciences, Docent, Department of Technology and Biotechnology of Meat and Meat Products</p><p>11, Volokolamskoe highway, Moscow, 125080</p><p>Tel.: +7–916–136–39–06</p></bio><email xlink:type="simple">BukhteevaYM@mgupp.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff xml:lang="en" id="aff-1"><institution>V. M. Gorbatov Federal Research Center for Food Systems</institution><country>Russian Federation</country></aff><aff xml:lang="en" id="aff-2"><institution>Russian Biotechnological University (ROSBIOTECH)</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>03</day><month>10</month><year>2024</year></pub-date><volume>9</volume><issue>3</issue><fpage>212</fpage><lpage>219</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Semenova A.A., Kuznetsova T.G., Seliverstova O.A., Salikova M.N., Spirina M.E., Bukhteeva Y.M., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Semenova A.A., Kuznetsova T.G., Seliverstova O.A., Salikova M.N., Spirina M.E., Bukhteeva Y.M.</copyright-holder><copyright-holder xml:lang="en">Semenova A.A., Kuznetsova T.G., Seliverstova O.A., Salikova M.N., Spirina M.E., Bukhteeva Y.M.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.meatjournal.ru/jour/article/view/375">https://www.meatjournal.ru/jour/article/view/375</self-uri><abstract><p>The use of pork obtained from intensively growing hybrid animals as the main raw material in meat processing, in particular sausage production, is accompanied by undesirable variability of consumer characteristics of sausage products and a decrease in the economic indicators of enterprise performance. The main technological disadvantages of processed pork are reduced water-binding capacity and significant loss of meat fluid, which in practice is usually compensated by the increased use of food additives and non-meat ingredients. The aim of the study was to assess the possibility of targeted improvement of the technological properties of minced pork using a proteolytic enzyme of animal origin. Model samples of minced meat were developed and cured for 24 hours at a temperature of 4 °C. The following control samples were used: minced meat with the addition of 2% edible salt and minced meat with the addition of 2% edible salt and 0.2% sodium bicarbonate. Moreover, 0.0001% chymotrypsin was added to the test samples containing similar curing ingredients. During electrophoretic study, in samples with the enzyme, an increase in low-molecular fractions (20 kDa, 15 kDa and lower) was observed, which indicated the manifestation of proteolytic activity of chymotrypsin in model systems. Enzymatic treatment led to an increase in pH and water-binding capacity. Cooking loss was reduced by 3 to 6 times, compared to Control 1. After cooking, histological studies of model systems showed that the test samples subjected to enzymatic treatment were characterized by a denser arrangement of structural elements, less pronounced cellular components of muscle tissue and the presence of glutin formed as a result of protein breakdown, filling the microcapillaries. Thus, the use of an enzyme preparation provides an opportunity for targeted improvement of the technological properties of pork obtained from intensively growing hybrid animals.</p></abstract><kwd-group xml:lang="en"><kwd>pork</kwd><kwd>minced pork</kwd><kwd>curing</kwd><kwd>enzymatic treatment</kwd><kwd>chymotrypsin</kwd><kwd>electrophoresis</kwd><kwd>water-binding capacity</kwd><kwd>pH</kwd><kwd>cooking loss</kwd></kwd-group><funding-group><funding-statement xml:lang="en">The article was published as part of the research topic No. FGUS-2024-0002 of the state assignment of the V. M. Gorbatov Federal Research Center for Food Systems of RAS</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Troncoso, F.D., Sánchez D. A., Ferreira, M.L. (2022). Production of plant proteases and new biotechnological applications: An updated review. ChemistryOpen, 11(3), Article e202200017. https://doi.org/10.1002/open.202200017</mixed-citation><mixed-citation xml:lang="en">Troncoso, F.D., Sánchez D. A., Ferreira, M.L. (2022). Production of plant proteases and new biotechnological applications: An updated review. ChemistryOpen, 11(3), Article e202200017. https://doi.org/10.1002/open.202200017</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Abril, B., Bou, R., García-Pérez, J.V., Benedito, J. (2023). Role of enzymatic reactions in meat processing and use of emerging technologies for process intensification. Foods, 12(10), Article 1940. https://doi.org/10.3390/foods12101940</mixed-citation><mixed-citation xml:lang="en">Abril, B., Bou, R., García-Pérez, J.V., Benedito, J. (2023). Role of enzymatic reactions in meat processing and use of emerging technologies for process intensification. Foods, 12(10), Article 1940. https://doi.org/10.3390/foods12101940</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Ouali, A., Herrera-Mendez, C.H., Coulis, G., Becila, S., Boudjellal, A., Aubry, L. et al. (2006). Revisiting the conversion of muscle into meat and the underlying mechanisms. Meat Science, 74(1), 44–58. https://doi.org/10.1016/j.meatsci.2006.05.010</mixed-citation><mixed-citation xml:lang="en">Ouali, A., Herrera-Mendez, C.H., Coulis, G., Becila, S., Boudjellal, A., Aubry, L. et al. (2006). Revisiting the conversion of muscle into meat and the underlying mechanisms. Meat Science, 74(1), 44–58. https://doi.org/10.1016/j.meatsci.2006.05.010</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Singh, P. K., Shrivastava, N., Ojha, B. K. (2019). Enzymes in the meat industry. Chapter in a book: Enzymes in food biotechnology. Academic Press, 2019. https://doi.org/10.1016/B978-0-12-813280-7.00008-6</mixed-citation><mixed-citation xml:lang="en">Singh, P. K., Shrivastava, N., Ojha, B. K. (2019). Enzymes in the meat industry. Chapter in a book: Enzymes in food biotechnology. Academic Press, 2019. https://doi.org/10.1016/B978-0-12-813280-7.00008-6</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Madhusankha, G.D.M.P., Thilakarathna, R.C.N. (2021). Meat tenderization mechanism and the impact of plant exogenous proteases: A review. Arabian Journal of Chemistry, 14(2), Article 102967. https://doi.org/10.1016/j.arajc.2020.102967</mixed-citation><mixed-citation xml:lang="en">Madhusankha, G.D.M.P., Thilakarathna, R.C.N. (2021). Meat tenderization mechanism and the impact of plant exogenous proteases: A review. Arabian Journal of Chemistry, 14(2), Article 102967. https://doi.org/10.1016/j.arajc.2020.102967</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Arshad, M. S., Kwon, J. -H., Imran, M., Sohaib, M., Aslam, A., Nawaz, I. et al. (2016). Plant and bacterial proteases: A key towards improving meat tenderization, a mini review. Cogent Food and Agriculture, 2(1), Article 1261780. https://doi.org/10.1080/23311932.2016.1261780</mixed-citation><mixed-citation xml:lang="en">Arshad, M. S., Kwon, J. -H., Imran, M., Sohaib, M., Aslam, A., Nawaz, I. et al. (2016). Plant and bacterial proteases: A key towards improving meat tenderization, a mini review. Cogent Food and Agriculture, 2(1), Article 1261780. https://doi.org/10.1080/23311932.2016.1261780</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Ha, M., Bekhit, A. El-D., Carne, A., Hopkins, D.L. (2013). Comparison of the proteolytic activities of new commercially available bacterial and fungal proteases toward meat proteins. Journal of Food Science, 78(2), C170-C177. https://doi.org/10.1111/1750–3841.12027</mixed-citation><mixed-citation xml:lang="en">Ha, M., Bekhit, A. El-D., Carne, A., Hopkins, D.L. (2013). Comparison of the proteolytic activities of new commercially available bacterial and fungal proteases toward meat proteins. Journal of Food Science, 78(2), C170-C177. https://doi.org/10.1111/1750–3841.12027</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Niamah, l.K., Al-Sahlany, S.T.G, Verma, D.K., Singh, S., Tripathy, S., Thakur, M. et al. (2024). Enzymes for meat and meat processing industry: Current trends, technological development, and future prospects. Chapter in a Book: Enzymatic Processes for Food Valorization. Academic Press, 2024. https://doi.org/10.1016/B978-0-323-95996-4.00002-2</mixed-citation><mixed-citation xml:lang="en">Niamah, l.K., Al-Sahlany, S.T.G, Verma, D.K., Singh, S., Tripathy, S., Thakur, M. et al. (2024). Enzymes for meat and meat processing industry: Current trends, technological development, and future prospects. Chapter in a Book: Enzymatic Processes for Food Valorization. Academic Press, 2024. https://doi.org/10.1016/B978-0-323-95996-4.00002-2</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Liu, R., Xing, L., Fu, Q., Zhou, G.-h., Zhang, W.-g. (2016). A Review of Antioxidant Peptides Derived from Meat Muscle and By-Products. Antioxidants, 5(3), Article 32. https://doi.org/10.3390/antiox5030032</mixed-citation><mixed-citation xml:lang="en">Liu, R., Xing, L., Fu, Q., Zhou, G.-h., Zhang, W.-g. (2016). A Review of Antioxidant Peptides Derived from Meat Muscle and By-Products. Antioxidants, 5(3), Article 32. https://doi.org/10.3390/antiox5030032</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Toldrá, F., Mora, L., Milagro Reig, M. (2016). New insights into meat by-product utilization. Meat Science, 120, 54–59. https://doi.org/10.1016/j.meatsci.2016.04.021</mixed-citation><mixed-citation xml:lang="en">Toldrá, F., Mora, L., Milagro Reig, M. (2016). New insights into meat by-product utilization. Meat Science, 120, 54–59. https://doi.org/10.1016/j.meatsci.2016.04.021</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Fiocchi, A., Restani, P., Riva, E., Restelli, A. R., Biasucci, G., Galli, C. L. et al. (1995). Meat allergy: II–Effects of food processing and enzymatic digestion on the allergenicity of bovine and ovine meats. Journal of the American College of Nutrition, 14(3), 245–250. https://doi.org/10.1080/07315724.1995.10718503</mixed-citation><mixed-citation xml:lang="en">Fiocchi, A., Restani, P., Riva, E., Restelli, A. R., Biasucci, G., Galli, C. L. et al. (1995). Meat allergy: II–Effects of food processing and enzymatic digestion on the allergenicity of bovine and ovine meats. Journal of the American College of Nutrition, 14(3), 245–250. https://doi.org/10.1080/07315724.1995.10718503</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Dong, X., Wang, J., Raghavan, V. (2020). Critical reviews and recent advances of novel non-thermal processing techniques on the modification of food allergens. Critical Reviews in Food Science and Nutrition, 61(2), 196–210. https://doi.org/10.1080/10408398.2020.1722942</mixed-citation><mixed-citation xml:lang="en">Dong, X., Wang, J., Raghavan, V. (2020). Critical reviews and recent advances of novel non-thermal processing techniques on the modification of food allergens. Critical Reviews in Food Science and Nutrition, 61(2), 196–210. https://doi.org/10.1080/10408398.2020.1722942</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Ponomarev, V. Ya., Yunusov, E. Sh., Ezhkova, G.O., Reshetnik, O.A. (2009). Biotechnological base for application of microbiological synthesis preparations for processing of meat raw materials with reduced functional and technological properties. Kazan: Kazan State Technological University, 2009. (In Russian)</mixed-citation><mixed-citation xml:lang="en">Ponomarev, V. Ya., Yunusov, E. Sh., Ezhkova, G.O., Reshetnik, O.A. (2009). Biotechnological base for application of microbiological synthesis preparations for processing of meat raw materials with reduced functional and technological properties. Kazan: Kazan State Technological University, 2009. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Lebret, B., Čandek-Potokar, M. (2022). Review: Pork quality attributes from farm to fork. Part I. Carcass and fresh meat. Animal, 16(Suppl 1), Article 100402, https://doi.org/10.1016/j.animal.2021.100402</mixed-citation><mixed-citation xml:lang="en">Lebret, B., Čandek-Potokar, M. (2022). Review: Pork quality attributes from farm to fork. Part I. Carcass and fresh meat. Animal, 16(Suppl 1), Article 100402, https://doi.org/10.1016/j.animal.2021.100402</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Semenova, A.A., Kuznetsova, T.G., Nasonova, V.V., Nekrasov, R.V., Bogolyubova, N.V. (2019). Myopathy as a destabilizing factor of meat quality formation. Theory and Practice of Meat Processing, 4(3), 24–31. https://doi.org/10.21323/2414-438X-2019-4-3-24-31</mixed-citation><mixed-citation xml:lang="en">Semenova, A.A., Kuznetsova, T.G., Nasonova, V.V., Nekrasov, R.V., Bogolyubova, N.V. (2019). Myopathy as a destabilizing factor of meat quality formation. Theory and Practice of Meat Processing, 4(3), 24–31. https://doi.org/10.21323/2414-438X-2019-4-3-24-31</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Lebret, B., Čandek-Potokar, M. (2022). Pork quality attributes from farm to fork. Part II. Processed pork products. Animal, 16, Article 100383. https://doi.org/10.1016/j.animal.2021.100383</mixed-citation><mixed-citation xml:lang="en">Lebret, B., Čandek-Potokar, M. (2022). Pork quality attributes from farm to fork. Part II. Processed pork products. Animal, 16, Article 100383. https://doi.org/10.1016/j.animal.2021.100383</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Vasilyeva, M.I., Zlobina, M.V., Kazantseva, N.P. (2021). Features of processing pork with different functional and technological characteristics peculiarities of pork processing with different functional-and-technological characteristics. The Bulletin of the Izhevsk State Agricultural Academy, 2(66), 16–23. https://doi.org/10.48012/1817-5457_2021_2_16 (In Russian)</mixed-citation><mixed-citation xml:lang="en">Vasilyeva, M.I., Zlobina, M.V., Kazantseva, N.P. (2021). Features of processing pork with different functional and technological characteristics peculiarities of pork processing with different functional-and-technological characteristics. The Bulletin of the Izhevsk State Agricultural Academy, 2(66), 16–23. https://doi.org/10.48012/1817-5457_2021_2_16 (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Teixeira, A., Rodrigues, S. (2021). Consumer perceptions towards healthier meat products. Current Opinion in Food Science, 38, 147–154. https://doi.org/10.1016/j.cofs.2020.12.004</mixed-citation><mixed-citation xml:lang="en">Teixeira, A., Rodrigues, S. (2021). Consumer perceptions towards healthier meat products. Current Opinion in Food Science, 38, 147–154. https://doi.org/10.1016/j.cofs.2020.12.004</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Liu J., Arner, A., Puolanne, E., Ertbjerg, P. (2016). On the water-holding of myofibrils: Effect of sarcoplasmic protein denaturation. Meat Science, 119, 32–40. https://doi.org/10.1016/j.meatsci.2016.04.020</mixed-citation><mixed-citation xml:lang="en">Liu J., Arner, A., Puolanne, E., Ertbjerg, P. (2016). On the water-holding of myofibrils: Effect of sarcoplasmic protein denaturation. Meat Science, 119, 32–40. https://doi.org/10.1016/j.meatsci.2016.04.020</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Mosolov, V.V. (1971). Proteolytic enzymes. Moscow: Nauka, 1971. (In Russian)</mixed-citation><mixed-citation xml:lang="en">Mosolov, V.V. (1971). Proteolytic enzymes. Moscow: Nauka, 1971. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Lee, S., Choi, Y. -S., Jo, K., Yong, H. I., Jeong, H. G., Jung, S. (2021). Improvement of meat protein digestibility in infants and the elderly. Food Chemistry, 356, Article 129707. https://doi.org/10.1016/j.foodchem.2021.129707</mixed-citation><mixed-citation xml:lang="en">Lee, S., Choi, Y. -S., Jo, K., Yong, H. I., Jeong, H. G., Jung, S. (2021). Improvement of meat protein digestibility in infants and the elderly. Food Chemistry, 356, Article 129707. https://doi.org/10.1016/j.foodchem.2021.129707</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685. https://doi.org/10.1038/227680a0</mixed-citation><mixed-citation xml:lang="en">Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685. https://doi.org/10.1038/227680a0</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Zhuravskaya, N.K., Alekhina, L.T., Otryashnikova, L.M. (1985). Research and quality control of meat and meat products. Moscow: Agropromizdat, 1985. (In Russian)</mixed-citation><mixed-citation xml:lang="en">Zhuravskaya, N.K., Alekhina, L.T., Otryashnikova, L.M. (1985). Research and quality control of meat and meat products. Moscow: Agropromizdat, 1985. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Zhang, R., Yoo, M.J., Farouk, M.M. (2021). Oxidative stability, proteolysis, and in vitro digestibility of fresh and long-term frozen stored in-bag dry-aged lean beef. Food Chemistry, 344, Article 128601. https://doi.org/10.1016/j.foodchem.2020.128601</mixed-citation><mixed-citation xml:lang="en">Zhang, R., Yoo, M.J., Farouk, M.M. (2021). Oxidative stability, proteolysis, and in vitro digestibility of fresh and long-term frozen stored in-bag dry-aged lean beef. Food Chemistry, 344, Article 128601. https://doi.org/10.1016/j.foodchem.2020.128601</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Lee, S., Jo, K., Jeong, H.G., Choi, Y.-S., Jung, S. (2023). Changes in beef protein digestibility in an in vitro infant digestion model with prefreezing temperatures and aging periods. Heliyon, 9(5), Article E15611. https://doi.org/10.1016/j.heliyon.2023.e15611</mixed-citation><mixed-citation xml:lang="en">Lee, S., Jo, K., Jeong, H.G., Choi, Y.-S., Jung, S. (2023). Changes in beef protein digestibility in an in vitro infant digestion model with prefreezing temperatures and aging periods. Heliyon, 9(5), Article E15611. https://doi.org/10.1016/j.heliyon.2023.e15611</mixed-citation></citation-alternatives></ref><ref id="cit26"><label>26</label><citation-alternatives><mixed-citation xml:lang="ru">Bhat, Z. F., Morton, J. D., Bekhit, A. El-D. A., Kumar, S., Bhat, H. F. (2021). Non-thermal processing has an impact on the digestibility of the muscle proteins. Critical Reviews in Food Science and Nutrition, 62(28), 7773–7800. https://doi.org/10.1080/10408398.2021.1918629</mixed-citation><mixed-citation xml:lang="en">Bhat, Z. F., Morton, J. D., Bekhit, A. El-D. A., Kumar, S., Bhat, H. F. (2021). Non-thermal processing has an impact on the digestibility of the muscle proteins. Critical Reviews in Food Science and Nutrition, 62(28), 7773–7800. https://doi.org/10.1080/10408398.2021.1918629</mixed-citation></citation-alternatives></ref><ref id="cit27"><label>27</label><citation-alternatives><mixed-citation xml:lang="ru">EFSA. (2021). Safety evaluation of a food enzyme containing trypsin and chymotrypsin from porcine pancreas. EFSA Journal, 19(1), Article e06369. https://doi.org/10.2903/j.efsa.2021.6369</mixed-citation><mixed-citation xml:lang="en">EFSA. (2021). Safety evaluation of a food enzyme containing trypsin and chymotrypsin from porcine pancreas. EFSA Journal, 19(1), Article e06369. https://doi.org/10.2903/j.efsa.2021.6369</mixed-citation></citation-alternatives></ref><ref id="cit28"><label>28</label><citation-alternatives><mixed-citation xml:lang="ru">Valetti, N.W., Lombardi, J., Boeris, V., Picó, G. (2012). Precipitation of chymotrypsin from fresh bovine pancreas using ι-carrageenan. Process Biochemistry, 47(12), 2570–2574. https://doi.org/10.1016/j.procbio.2012.09.021</mixed-citation><mixed-citation xml:lang="en">Valetti, N.W., Lombardi, J., Boeris, V., Picó, G. (2012). Precipitation of chymotrypsin from fresh bovine pancreas using ι-carrageenan. Process Biochemistry, 47(12), 2570–2574. https://doi.org/10.1016/j.procbio.2012.09.021</mixed-citation></citation-alternatives></ref><ref id="cit29"><label>29</label><citation-alternatives><mixed-citation xml:lang="ru">Krasnoshtanova, A.A., Besyaeva, A.D. (2022). A comparative characterization of the properties of biocatalysts containing chymotrypsin immobilized on polysaccharide supports. Catalysis in Industry, 22(4), 75–81. https://doi.org/10.18412/1816-0387-2022-4-75-81 (In Russian)</mixed-citation><mixed-citation xml:lang="en">Krasnoshtanova, A.A., Besyaeva, A.D. (2022). A comparative characterization of the properties of biocatalysts containing chymotrypsin immobilized on polysaccharide supports. Catalysis in Industry, 22(4), 75–81. https://doi.org/10.18412/1816-0387-2022-4-75-81 (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit30"><label>30</label><citation-alternatives><mixed-citation xml:lang="ru">Gurumallesh, P., Alagu, K., Ramakrishnan, B., Muthusamy, S. (2019). A systematic reconsideration on proteases. International Journal of Biological Macromolecules, 128, 254–267. https://doi.org/10.1016/j.ijbiomac.2019.01.081</mixed-citation><mixed-citation xml:lang="en">Gurumallesh, P., Alagu, K., Ramakrishnan, B., Muthusamy, S. (2019). A systematic reconsideration on proteases. International Journal of Biological Macromolecules, 128, 254–267. https://doi.org/10.1016/j.ijbiomac.2019.01.081</mixed-citation></citation-alternatives></ref><ref id="cit31"><label>31</label><citation-alternatives><mixed-citation xml:lang="ru">Zeng, Z., Li, C., Ertbjerg, P. (2017). Relationship between proteolysis and water-holding of myofibrils. Meat Science, 131, 48–55. https://doi.org/10.1016/j.meatsci.2017.04.232</mixed-citation><mixed-citation xml:lang="en">Zeng, Z., Li, C., Ertbjerg, P. (2017). Relationship between proteolysis and water-holding of myofibrils. Meat Science, 131, 48–55. https://doi.org/10.1016/j.meatsci.2017.04.232</mixed-citation></citation-alternatives></ref><ref id="cit32"><label>32</label><citation-alternatives><mixed-citation xml:lang="ru">Liu J., Arner A., Puolanne E., Ertbjerg P. (2016). On the water-holding of myofibrils: Effect of sarcoplasmic protein denaturation. Meat Science, 119, 32–40, https://doi.org/10.1016/j.meatsci.2016.04.020</mixed-citation><mixed-citation xml:lang="en">Liu J., Arner A., Puolanne E., Ertbjerg P. (2016). On the water-holding of myofibrils: Effect of sarcoplasmic protein denaturation. Meat Science, 119, 32–40, https://doi.org/10.1016/j.meatsci.2016.04.020</mixed-citation></citation-alternatives></ref><ref id="cit33"><label>33</label><citation-alternatives><mixed-citation xml:lang="ru">Xiong L. (2018). Muscle proteins. Chapter in a book: Proteins in Food Processing. Woodhead Publishing, 2018. https://doi.org/10.1016/B978-0-08-100722-8.00006-1</mixed-citation><mixed-citation xml:lang="en">Xiong L. (2018). Muscle proteins. Chapter in a book: Proteins in Food Processing. Woodhead Publishing, 2018. https://doi.org/10.1016/B978-0-08-100722-8.00006-1</mixed-citation></citation-alternatives></ref><ref id="cit34"><label>34</label><citation-alternatives><mixed-citation xml:lang="ru">Amiri A., Sharifian P., Morakabati N., Mousakhani-Ganjeh A., Mirtaheri M., Nilghaz A. et al. (2021). Modification of functional, rheological and structural characteristics of myofibrillar proteins by high-intensity ultrasonic and papain treatment. Innovative Food Science and Emerging Technologies, 72, Article 102748. https://doi.org/10.1016/j.ifset.2021.102748</mixed-citation><mixed-citation xml:lang="en">Amiri A., Sharifian P., Morakabati N., Mousakhani-Ganjeh A., Mirtaheri M., Nilghaz A. et al. (2021). Modification of functional, rheological and structural characteristics of myofibrillar proteins by high-intensity ultrasonic and papain treatment. Innovative Food Science and Emerging Technologies, 72, Article 102748. https://doi.org/10.1016/j.ifset.2021.102748</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
