meatTheory and practice of meat processingТеория и практика переработки мяса2414-438X2414-441XФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН10.21323/2414-438X-2023-8-2-112-123meat-263Research ArticleСтатьиSalmonella enterica species isolated from local foodstuff and patients suffering from foodborne illness: Surveillance, antimicrobial resistance and molecular detectionhttps://orcid.org/0000-0002-0193-9218AlShaheebZ. A.

Zahraa A. AlShaheeb, MSc, Assistant lecturer, Laboratory Scientist

Baghdad 64074

Tel.:+9647830602282

zahraa.abdulkareem@ced.nahrainuniv.edu.iqhttps://orcid.org/0000-0002-3431-7562ThabitZ. A.

Zaid A. Thabit, PhD, Assistant professor, Academic Stuff, Environmental Biotech Department, Biotechnology Research Center

Baghdad 64074

Tel.:+9647700326685

zaid.alrawi@brc.nahrainuniv.edu.iqhttps://orcid.org/0000-0002-0533-1349OraibiA. G.

Asma G. Oraibi, PhD, Professor, Academic Stuff, Plant Biotechnology Department, College of Biotechnology

Baghdad 64074

Tel.: +9647819905976

asma.ghatea@nahrainuniv.edu.iqhttps://orcid.org/0000-0002-2909-603XBaioumyA. A.

Ahmed A. Baioumy, PhD, Assistant Professor, Food Science Department, Faculty of Agriculture

12613, 1 Gamaa Street, Giza

Tel.: +2–0122–838–44–78

ahmedadel35@agr.cu.edu.eghttps://orcid.org/0000-0002-7012-6667AbedelmaksoudT. G.

Tarek G. Abedelmaksoud, PhD, Assistant Professor, Food Science Department, Faculty of Agriculture

12613, 1 Gamaa Street, Giza

Tel.: +2–0110–144–12–80

tareekgamal_88@agr.cu.edu.egIraqi Ministry of HealthIraqAl-Nahrain UniversityIraqCairo UniversityEgypt20230807202382112123Copyright © AlShaheeb Z.A., Thabit Z.A., Oraibi A.G., Baioumy A.A., Abedelmaksoud T.G., 20232023AlShaheeb Z.A., Thabit Z.A., Oraibi A.G., Baioumy A.A., Abedelmaksoud T.G.AlShaheeb Z.A., Thabit Z.A., Oraibi A.G., Baioumy A.A., Abedelmaksoud T.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.meatjournal.ru/jour/article/view/263

The aim of this study was to determine the prevalence of Salmonella enterica in raw chicken meat, eggs, and ready-to-eat foods containing poultry products and among patients suffering from diarrhea as a result of ingestion of this foodborne pathogen in Baghdad, Iraq. It assesses the antibiotics susceptibility, virulence and pathogenicity of S. enterica isolates. Thirteen Salmonella spp. isolates from foodstuff and seven from clinical patients were recovered from 80 and 20 samples, respectively. Isolates from foodstuff samples displayed the highest resistance to nalidixic acid (69.23%), followed by chloramphenicol (53.84%). Salmonella spp. isolated from clinical samples showed resistance to both azithromycin and cefotaxime at the same percentage level (71.42%). The results of antibiotic resistance gene amplification (gyrA, mphA) were analyzed and showed that these genes were present in 100% and 50% of phenotypically resistant isolates, respectively. Virulence genes invA, avrA, and sipB were found on average in 86% of food isolates, accounting for 69.2%, 92.3%, and 95%, respectively. In addition, the detection of these virulence genes among clinical isolates showed their presence at the same level (85.7%). Our study revealed that unhygienic chicken slaughterhouses and lack of food safety management are strong indicators of a high probability of the Salmonella presence in our food products in the Iraqi markets.

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The authors declare that there are no conflicts of interest present.