meatTheory and practice of meat processingТеория и практика переработки мяса2414-438X2414-441XФГБНУ «Федеральный научный центр пищевых систем им. В.М. Горбатова» РАН10.21323/2414-438X-2022-7-1-35-41meat-208Research ArticleСтатьиComparison of the proteomic profile of pork byproducts during their storageComparison of the proteomic profile of pork byproducts during their storagehttps://orcid.org/0000-0002-0211-8171AkhremkoA. G.AkhremkoA. G.

Moscow

Anastasia G. Akhremko, Junior Researcher, Experimental-clinical research laboratory of bioactive substances of animal origin

26, Talalikhina str., 109316, Moscow

Tel.: +7–915–237–94–97

a.ahremko@fncps.ruhttps://orcid.org/0000-0001-7625-3838NasonovaV. V.NasonovaV. V.

Moscow

Victoria V. Nasonova, Candidate of technical sciences, Head of Department of Applied Scientific and Technological Development

26, Talalikhina str., 109316, Moscow

Tel.: +7–495–676–95–11 (307)

v.nasonova@fncps.ruhttps://orcid.org/0000-0003-4544-4433SpirinaM. E.SpirinaM. E.

Moscow

Maria E. Spirina, bachelor

49, Timiryazevskaya str., 49, 127550, Moscow

Tel.: + 7–977–541–27–97

mspirina88@gmail.comhttps://orcid.org/0000-0002-6876-8847GodswillN. N.GodswillN. N.

Yaounde

Ntsomboh N. Godswill, Doctor, PhD, Senior Lecturer, Department of Plant Biology, Faculty of Science; Head of the Department of Agricultural Biotechnology; Member 

P.O. Box 812, Yaounde

Tel.: +237-679-941-910

ntsomboh.godswill@facsciences-uy1.cmV.M. Gorbatov Federal Research Center for Food SystemsРоссияV.M. Gorbatov Federal Research Center for Food SystemsRussian FederationRussian State Agrarian University — Moscow Timiryazev Agricultural AcademyРоссияRussian State Agrarian University — Moscow Timiryazev Agricultural AcademyRussian FederationUniversity Yaounde I; FINISTECH; Cameroon Academy of Young ScientistsКамерунUniversity Yaounde I; FINISTECH; Cameroon Academy of Young ScientistsCameroon202212042022713541Copyright © Akhremko A.G., Nasonova V.V., Spirina M.E., Godswill N.N., 20222022Akhremko A.G., Nasonova V.V., Spirina M.E., Godswill N.N.Akhremko A.G., Nasonova V.V., Spirina M.E., Godswill N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.meatjournal.ru/jour/article/view/208

In this article, the proteomic profiles of pork by-products (snout, tongue, liver, kidney, spleen) were studied by comparative method on the first day and the fifth day of their storage. Two-dimensional electrophoresis according to O’Farrell was used for the aims of this article, while the results were further processed in ImageMaster software. Proteomic maps of by-products showed clear changes in protein composition after visualization and images analysis. There was a decrease and increase in manifestation intensity of some proteins. The study of the obtained electrophoregrams with the help of references resources allowed identifying various compounds in the by-products. 9 protein fractions with various intensity of manifestation were found on the day 1st and 5th. On the 1st day the following substances were intensively manifested: in the liver — glutathione peroxidase 4 (22.3 kDa), LEAP-2 (8.8 kDa); in the kidneys — quinone oxidoreductase (34.9 kDa); in the spleen — glycoprotein CD59 (13.7 kDa), in the patch — protein flint (49.07 kDa). It is noted that these proteins play their role in stopping certain processes in cells, like oxidation, microbial activity, and accumulation of toxic substances. These processes can worsen the quality of raw materials, and further lead to spoilage of the food product. On the 5th day of storage the highest intensity of manifestation of glyceraldehyde-3-phosphate dehydrogenase (35.8 kDa) in the liver was observed; superoxide dismutase [Cu-Zn] (15.8 kDa) was noted in the kidneys, colony-stimulating factor (16.2 kDa) was observed in the spleen and glutaredoxin –1 (11.8 kDa) in the tongue. In its turn, on the fifth day these chemical processes manifested themselves more intensely, as the fatty acids and glucose broke down. To obtain more accurate results, the proteins were compared by their volume. Among the identified fractions the highest expression was observed in LEAP 2 (8.8 kDa) on the first day, and in glyceraldehyde-3-phosphate dehydrogenase (35.8 kDa) on the fifth day. The least change in the intensity of manifestation was noted for superoxide dismutase [Cu-Zn] (15.8 kDa), which volume increased during storage by 13% for 5 days. The analysis of the obtained electrophoregrams allowed identifying various compounds, tracing the changes in the qualitative composition of protein in by-products during various periods of their storage. The obtained data demonstrate the transformation of protein molecules during storage, which makes it possible to determine the changes and quality of the food products.

In this article, the proteomic profiles of pork by-products (snout, tongue, liver, kidney, spleen) were studied by comparative method on the first day and the fifth day of their storage. Two-dimensional electrophoresis according to O’Farrell was used for the aims of this article, while the results were further processed in ImageMaster software. Proteomic maps of by-products showed clear changes in protein composition after visualization and images analysis. There was a decrease and increase in manifestation intensity of some proteins. The study of the obtained electrophoregrams with the help of references resources allowed identifying various compounds in the by-products. 9 protein fractions with various intensity of manifestation were found on the day 1st and 5th. On the 1st day the following substances were intensively manifested: in the liver — glutathione peroxidase 4 (22.3 kDa), LEAP-2 (8.8 kDa); in the kidneys — quinone oxidoreductase (34.9 kDa); in the spleen — glycoprotein CD59 (13.7 kDa), in the patch — protein flint (49.07 kDa). It is noted that these proteins play their role in stopping certain processes in cells, like oxidation, microbial activity, and accumulation of toxic substances. These processes can worsen the quality of raw materials, and further lead to spoilage of the food product. On the 5th day of storage the highest intensity of manifestation of glyceraldehyde-3-phosphate dehydrogenase (35.8 kDa) in the liver was observed; superoxide dismutase [Cu-Zn] (15.8 kDa) was noted in the kidneys, colony-stimulating factor (16.2 kDa) was observed in the spleen and glutaredoxin –1 (11.8 kDa) in the tongue. In its turn, on the fifth day these chemical processes manifested themselves more intensely, as the fatty acids and glucose broke down. To obtain more accurate results, the proteins were compared by their volume. Among the identified fractions the highest expression was observed in LEAP 2 (8.8 kDa) on the first day, and in glyceraldehyde-3-phosphate dehydrogenase (35.8 kDa) on the fifth day. The least change in the intensity of manifestation was noted for superoxide dismutase [Cu-Zn] (15.8 kDa), which volume increased during storage by 13% for 5 days. The analysis of the obtained electrophoregrams allowed identifying various compounds, tracing the changes in the qualitative composition of protein in by-products during various periods of their storage. The obtained data demonstrate the transformation of protein molecules during storage, which makes it possible to determine the changes and quality of the food products.

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The authors declare that there are no conflicts of interest present.